Gene/Proteome Database (LMPD)
LMPD ID
LMP005246
Gene ID
Species
Homo sapiens (Human)
Gene Name
carboxylesterase 1
Gene Symbol
Synonyms
ACAT; CE-1; CEH; CES2; HMSE; HMSE1; PCE-1; REH; SES1; TGH; hCE-1
Alternate Names
liver carboxylesterase 1; egasyn; serine esterase 1; retinyl ester hydrolase; cocaine carboxylesterase; triacylglycerol hydrolase; carboxylesterase 2 (liver); brain carboxylesterase hBr1; cholesteryl ester hydrolase; monocyte/macrophage serine esterase; methylumbelliferyl-acetate deacetylase 1; acyl coenzyme A:cholesterol acyltransferase; acyl-coenzyme A:cholesterol acyltransferase; human monocyte/macrophage serine esterase 1; carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Chromosome
16
Map Location
16q22.2
EC Number
3.1.1.1
Summary
This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Orthologs
Proteins
| liver carboxylesterase 1 isoform a precursor | |
|---|---|
| Refseq ID | NP_001020366 |
| Protein GI | 68508967 |
| UniProt ID | P23141 |
| mRNA ID | NM_001025195 |
| Length | 568 |
| RefSeq Status | REVIEWED |
| MWLRAFILATLSASAAWAGHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMQLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL | |
| liver carboxylesterase 1 isoform b precursor | |
|---|---|
| Refseq ID | NP_001020365 |
| Protein GI | 68508965 |
| UniProt ID | P23141 |
| mRNA ID | NM_001025194 |
| Length | 567 |
| RefSeq Status | REVIEWED |
| MWLRAFILATLSASAAWGHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMQLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL | |
| liver carboxylesterase 1 isoform c precursor | |
|---|---|
| Refseq ID | NP_001257 |
| Protein GI | 68508957 |
| UniProt ID | P23141 |
| mRNA ID | NM_001266 |
| Length | 566 |
| RefSeq Status | REVIEWED |
| MWLRAFILATLSASAAWGHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL | |
| sig_peptide: 1..18 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 1965 peptide sequence: MWLRAFILATLSASAAWG mat_peptide: 19..567 product: liver carboxylesterase 1 isoform b calculated_mol_wt: 60574 peptide sequence: HPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMQLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL sig_peptide: 1..18 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 1979 peptide sequence: MWLRAFILATLSASAAWA mat_peptide: 19..568 product: liver carboxylesterase 1 isoform a calculated_mol_wt: 60631 peptide sequence: GHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMQLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL sig_peptide: 1..18 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 1965 peptide sequence: MWLRAFILATLSASAAWG mat_peptide: 19..566 product: liver carboxylesterase 1 isoform c calculated_mol_wt: 60446 peptide sequence: HPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IEA:UniProtKB-KW | C | endoplasmic reticulum |
| GO:0052689 | TAS:ProtInc | F | carboxylic ester hydrolase activity |
| GO:0047374 | IEA:UniProtKB-EC | F | methylumbelliferyl-acetate deacetylase activity |
| GO:0030855 | IDA:UniProt | P | epithelial cell differentiation |
| GO:0008152 | TAS:ProtInc | P | metabolic process |
| GO:0009636 | TAS:ProtInc | P | response to toxic substance |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa00983 | Drug metabolism - other enzymes |
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=P23141-1; Sequence=Displayed; Name=2; IsoId=P23141-2; Sequence=VSP_021026; Name=3; IsoId=P23141-3; Sequence=VSP_047158; |
| Biophysicochemical Properties | Kinetic parameters: KM=106.6 uM for p-nitrophenyl acetate {ECO |
| Catalytic Activity | 4-methylumbelliferyl acetate + H(2)O = 4- methylumbelliferone + acetate. |
| Catalytic Activity | A carboxylic ester + H(2)O = an alcohol + a carboxylate. |
| Enzyme Regulation | Activated by CHAPS. |
| Function | Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. {ECO |
| Ptm | Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. |
| Ptm | Contains sialic acid. |
| Sequence Caution | Sequence=AAA83932.1; Type=Frameshift; Positions=301, 312, 318, 383, 391; Evidence= ; |
| Similarity | Belongs to the type-B carboxylesterase/lipase family. |
| Subcellular Location | Endoplasmic reticulum lumen . |
| Subunit | Homotrimer and homohexamer. Binds to beta-glucuronidase. {ECO |
| Tissue Specificity | Expressed predominantly in liver with lower levels in heart and lung. |
Identical and Related Proteins
Unique RefSeq proteins for LMP005246 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 68508967 | RefSeq | NP_001020366 | 568 | liver carboxylesterase 1 isoform a precursor |
| 68508965 | RefSeq | NP_001020365 | 567 | liver carboxylesterase 1 isoform b precursor |
| 68508957 | RefSeq | NP_001257 | 566 | liver carboxylesterase 1 isoform c precursor |
Identical Sequences to LMP005246 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:68508965 | DBBJ | BAC87748.1 | 567 | carboxylesterase HU1a [Homo sapiens] |
| GI:68508965 | DBBJ | BAC87750.1 | 567 | carboxylesterase HU1a [Homo sapiens] |
| GI:68508965 | GenBank | AAA35649.1 | 567 | carboxylesterase [Homo sapiens] |
| GI:68508965 | GenBank | AAI10339.1 | 567 | Carboxylesterase 1 (monocyte/macrophage serine esterase 1) [Homo sapiens] |
| GI:68508957 | GenBank | AED65300.1 | 566 | Sequence 1 from patent US 7906637 |
| GI:68508957 | GenBank | AFD38750.1 | 566 | Sequence 1 from patent US 8124393 |
| GI:68508967 | GenBank | AGD76150.1 | 568 | Sequence 74 from patent US 8349555 |
| GI:68508965 | GenBank | AHE01140.1 | 567 | Sequence 56056 from patent US 8586006 |
| GI:68508967 | GenBank | AHE01141.1 | 568 | Sequence 56057 from patent US 8586006 |
| GI:68508957 | GenBank | AHE01142.1 | 566 | Sequence 56058 from patent US 8586006 |
| GI:68508965 | SwissProt | P23141.2 | 567 | RecName: Full=Liver carboxylesterase 1; AltName: Full=Acyl-coenzyme A:cholesterol acyltransferase; Short=ACAT; AltName: Full=Brain carboxylesterase hBr1; AltName: Full=Carboxylesterase 1; Short=CE-1; Short=hCE-1; AltName: Full=Cocaine carboxylesterase; AltName: Full=Egasyn; AltName: Full=HMSE; AltName: Full=Methylumbelliferyl-acetate deacetylase 1; AltName: Full=Monocyte/macrophage serine esterase; AltName: Full=Retinyl ester hydrolase; Short=REH; AltName: Full=Serine esterase 1; AltName: Full=Triacylglycerol hydrolase; Short=TGH; Flags: Precursor [Homo sapiens] |
Related Sequences to LMP005246 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:68508967 | DBBJ | BAC87748.1 | 567 | carboxylesterase HU1a [Homo sapiens] |
| GI:68508957 | DBBJ | BAC87748.1 | 567 | carboxylesterase HU1a [Homo sapiens] |
| GI:68508967 | DBBJ | BAC87750.1 | 567 | carboxylesterase HU1a [Homo sapiens] |
| GI:68508957 | DBBJ | BAC87750.1 | 567 | carboxylesterase HU1a [Homo sapiens] |
| GI:68508967 | GenBank | AAA16036.1 | 568 | carboxylesterase [Homo sapiens] |
| GI:68508957 | GenBank | AAA35711.1 | 566 | carboxylesterase [Homo sapiens] |
| GI:68508965 | GenBank | AAD53175.1 | 567 | egasyn [Homo sapiens] |
| GI:68508965 | GenBank | AAC60631.2 | 568 | acyl coenzyme A:cholesterol acyltransferase [Homo sapiens] |
| GI:68508967 | GenBank | AAC60631.2 | 568 | acyl coenzyme A:cholesterol acyltransferase [Homo sapiens] |
| GI:68508965 | GenBank | AAP20868.1 | 567 | cholesteryl ester hydrolase [Homo sapiens] |
| GI:68508957 | GenBank | AAY03030.1 | 566 | Sequence 1 from patent US 6861233 |
| GI:68508965 | GenBank | AGD76150.1 | 568 | Sequence 74 from patent US 8349555 |
| GI:68508967 | GenBank | AHE01140.1 | 567 | Sequence 56056 from patent US 8586006 |
| GI:68508957 | GenBank | AHE01140.1 | 567 | Sequence 56056 from patent US 8586006 |
| GI:68508965 | GenBank | AHE01141.1 | 568 | Sequence 56057 from patent US 8586006 |
| GI:68508957 | RefSeq | NP_001020365.1 | 567 | liver carboxylesterase 1 isoform b precursor [Homo sapiens] |
| GI:68508967 | RefSeq | NP_001020365.1 | 567 | liver carboxylesterase 1 isoform b precursor [Homo sapiens] |
| GI:68508965 | RefSeq | NP_001020366.1 | 568 | liver carboxylesterase 1 isoform a precursor [Homo sapiens] |