Gene/Proteome Database (LMPD)

LMPD ID
LMP005381
Gene ID
55501
Species
Homo sapiens (Human)
Gene Name
carbohydrate (chondroitin 4) sulfotransferase 12
Gene Symbol
CHST12
Synonyms
C4S-2; C4ST-2; C4ST2
Alternate Names
carbohydrate sulfotransferase 12; sulfotransferase Hlo; chondroitin 4-sulfotransferase 2; chondroitin 4-O-sulfotransferase 2
Chromosome
7
Map Location
7p22
EC Number
2.8.2.5
Summary
The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. Alternatively spliced transcript variants differing only in their 5' UTRs have been found for this gene. [provided by RefSeq, Aug 2011]
Orthologs
View orthologs and multiple alignments for CHST12

Proteins

carbohydrate sulfotransferase 12
Refseq ID NP_001230723
Protein GI 345090972
UniProt ID Q9NRB3
mRNA ID NM_001243794
Length 414
RefSeq Status REVIEWED
MTKARLFRLWLVLGSVFMILLIIVYWDSAGAAHFYLHTSFSRPHTGPPLPTPGPDRDRELTADSDVDEFLDKFLSAGVKQSDLPRKETEQPPAPGSMEESVRGYDWSPRDARRSPDQGRQQAERRSVLRGFCANSSLAFPTKERAFDDIPNSELSHLIVDDRHGAIYCYVPKVACTNWKRVMIVLSGSLLHRGAPYRDPLRIPREHVHNASAHLTFNKFWRRYGKLSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPMLRLYANHTSLPASAREAFRAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQIDYDFVGKLETLDEDAAQLLQLLQVDRQLRFPPSYRNRTASSWEEDWFAKIPLAWRQQLYKLYEADFVLFGYPKPENLLRD
carbohydrate sulfotransferase 12
Refseq ID NP_001230724
Protein GI 345090974
UniProt ID Q9NRB3
mRNA ID NM_001243795
Length 414
RefSeq Status REVIEWED
Protein sequence is identical to GI:345090972 (mRNA isoform)
carbohydrate sulfotransferase 12
Refseq ID NP_061111
Protein GI 8922112
UniProt ID Q9NRB3
mRNA ID NM_018641
Length 414
RefSeq Status REVIEWED
Protein sequence is identical to GI:345090972 (mRNA isoform)

Gene Information

Entrez Gene ID
55501
Gene Name
carbohydrate (chondroitin 4) sulfotransferase 12
Gene Symbol
CHST12
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000139 TAS:Reactome C Golgi membrane
GO:0030173 NAS:UniProtKB C integral component of Golgi membrane
GO:0016020 IDA:UniProtKB C membrane
GO:0050656 NAS:UniProtKB F 3'-phosphoadenosine 5'-phosphosulfate binding
GO:0047756 IDA:UniProtKB F chondroitin 4-sulfotransferase activity
GO:0016051 IEA:InterPro P carbohydrate biosynthetic process
GO:0005975 TAS:Reactome P carbohydrate metabolic process
GO:0030206 IDA:UniProtKB P chondroitin sulfate biosynthetic process
GO:0030204 TAS:Reactome P chondroitin sulfate metabolic process
GO:0030208 IDA:UniProtKB P dermatan sulfate biosynthetic process
GO:0030203 TAS:Reactome P glycosaminoglycan metabolic process
GO:0044281 TAS:Reactome P small molecule metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
hsa00532 Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6567 chondroitin sulfate biosynthesis (late stages)

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_120989 Chondroitin sulfate biosynthesis
REACT_121206 Chondroitin sulfate/dermatan sulfate metabolism

Domain Information

InterPro Annotations

Accession Description
IPR018011 Carbohydrate sulfotransferase-related
IPR005331 Sulfotransferase

UniProt Annotations

Entry Information

Gene Name
carbohydrate (chondroitin 4) sulfotransferase 12
Protein Entry
CHSTC_HUMAN
UniProt ID
Q9NRB3
Species
Human

Comments

Comment Type Description
Catalytic Activity 3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate.
Function Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.
Interaction Q9NYU2:UGGT1; NbExp=1; IntAct=EBI-1042837, EBI-1056389;
Similarity Belongs to the sulfotransferase 2 family.
Subcellular Location Golgi apparatus membrane ; Single-pass type II membrane protein .
Tissue Specificity Widely expressed. Expressed a high level in spinal chord, heart, spleen, thyroid, pituitary gland, adrenal gland, peripheral blood leukocytes, thymus, lung, small intestine, fetal kidney, fetal spleen and fetal lung.

Identical and Related Proteins

Unique RefSeq proteins for LMP005381 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
345090972 RefSeq NP_001230723 414 carbohydrate sulfotransferase 12

Identical Sequences to LMP005381 proteins

Reference Database Accession Length Protein Name
GI:345090972 GenBank ADM02159.1 414 Sequence 466 from patent US 7723488
GI:345090972 GenBank AFL59769.1 414 Sequence 418 from patent US 8106156
GI:345090972 GenBank AFO09858.1 414 Sequence 14057 from patent US 8221983
GI:345090972 GenBank AIC51781.1 414 CHST12, partial [synthetic construct]
GI:345090972 RefSeq NP_001230724.1 414 carbohydrate sulfotransferase 12 [Homo sapiens]
GI:345090972 RefSeq XP_004045078.1 414 PREDICTED: carbohydrate sulfotransferase 12 [Gorilla gorilla gorilla]

Related Sequences to LMP005381 proteins

Reference Database Accession Length Protein Name
GI:345090972 DBBJ BAD96911.1 414 carbohydrate (chondroitin 4) sulfotransferase 12 variant, partial [Homo sapiens]
GI:345090972 GenBank AAN97617.1 415 Sequence 133 from patent US 6476195
GI:345090972 GenBank ACE20397.1 414 Sequence 5627 from patent US 7368531
GI:345090972 GenBank ACH31148.1 414 Sequence 5627 from patent US 7411051
GI:345090972 RefSeq XP_003318271.1 414 PREDICTED: carbohydrate sulfotransferase 12 [Pan troglodytes]
GI:345090972 RefSeq XP_003318272.1 414 PREDICTED: carbohydrate sulfotransferase 12 [Pan troglodytes]