Gene/Proteome Database (LMPD)

LMPD ID
LMP005459
Gene ID
77337
Species
Mus musculus (Mouse)
Gene Name
aldo-keto reductase family 1, member C21
Gene Symbol
Akr1c21
Synonyms
9430025F20Rik; AI315367
Alternate Names
aldo-keto reductase family 1 member C21; DD1; DD3; 17-alpha-HSD; dihydrodiol dehydrogenase type 1; dihydrodiol dehydrogenase type 3; 3-alpha-hydroxysteroid dehydrogenase; 17-alpha-hydroxysteroid dehydrogenase; 3(17)alpha-hydroxysteroid dehydrogenase; 3(or 17)-alpha-hydroxysteroid dehydrogenase
Chromosome
13
Map Location
13 A1|13
EC Number
1.1.1.-

Proteins

aldo-keto reductase family 1 member C21
Refseq ID NP_084177
Protein GI 126090770
UniProt ID Q91WR5
mRNA ID NM_029901
Length 323
RefSeq Status VALIDATED
MNSKCHCVILNDGNFIPVLGFGTALPLECPKSKAKELTKIAIDAGFHHFDSASVYNTEDHVGEAIRSKIADGTVRREDIFYTSKVWCTSLHPELVRASLERSLQKLQFDYVDLYLIHYPMALKPGEENFPVDEHGKLIFDRVDLCATWEAMEKCKDAGLTKSIGVSNFNYRQLEMILNKPGLKYKPVCNQVECHPYLNQMKLLDFCKSKDIVLVAYGVLGTQRYGGWVDQNSPVLLDEPVLGSMAKKYNRTPALIALRYQLQRGIVVLNTSLKEERIKENMQVFEFQLSSEDMKVLDGLNRNMRYIPAAIFKGHPNWPFLDEY

Gene Information

Entrez Gene ID
77337
Gene Name
aldo-keto reductase family 1, member C21
Gene Symbol
Akr1c21
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0004033 ISA:MGI F aldo-keto reductase (NADP) activity
GO:0047023 IDA:UniProtKB F androsterone dehydrogenase activity
GO:0033764 IDA:MGI F steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0006694 ISA:MGI P steroid biosynthetic process

REACTOME Pathway Links

REACTOME Pathway ID Description
5893902 Retinoid metabolism and transport
5893443 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
5893439 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
5893441 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol

Domain Information

InterPro Annotations

Accession Description
IPR001395 Aldo/keto reductase
IPR020471 Aldo/keto reductase subgroup
IPR018170 Aldo/keto reductase, conserved site
IPR023210 NADP-dependent oxidoreductase domain

UniProt Annotations

Entry Information

Gene Name
aldo-keto reductase family 1, member C21
Protein Entry
AK1CL_MOUSE
UniProt ID
Q91WR5
Species
Mouse

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=1.8 uM for NADP {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=19 uM for androstenedione {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.5 uM for 5-beta-pregnane-3-alpha,20-alpha-diol {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.5 uM for 5-beta-pregnane-3,20-dione {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.6 uM for epitestosterone {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.3 uM for androst-4-ene-3,17-dione {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; pH dependence: Optimum pH is 10.0. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338};
Catalytic Activity Androsterone + NAD(P)(+) = 5-alpha-androstane- 3,17-dione + NAD(P)H. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:20124700}.
Enzyme Regulation Inhibited by high concentrations of substrate. {ECO:0000269|PubMed:15577209}.
Function NADP-dependent 17-alpha-hydroxysteroid dehydrogenase that converts 5-alpha-androstane-3,17-dione into androsterone. Has lower 3-alpha-hydroxysteroid dehydrogenase activity. Has broad substrate specificity and acts on various 17-alpha- hydroxysteroids, 17-ketosteroids, 3-alpha hydroxysteroids and 3- ketosteroids. Reduction of keto groups is strictly stereoselective. Reduction of 17-ketosteroids yields only 17- alpha-hydroxysteroids. Likewise, reduction of 3-ketosteroids yields only 3-alpha-hydroxysteroids. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:16018803, ECO:0000269|PubMed:17034817, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:20124700}.
Similarity Belongs to the aldo/keto reductase family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000250}.
Subunit Monomer. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17034817, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:17909281, ECO:0000269|PubMed:19237748}.
Tissue Specificity Detected in kidney and brain. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:16018803}.

Identical and Related Proteins

Unique RefSeq proteins for LMP005459 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
126090770 RefSeq NP_084177 323 aldo-keto reductase family 1 member C21

Identical Sequences to LMP005459 proteins

Reference Database Accession Length Protein Name
GI:126090770 PDB 2HE8 323 Chain B, Crystal Structure Of 17alpha-Hydroxysteroid Dehydrogenase In Its Apo- Form
GI:126090770 PDB 2HEJ 323 Chain A, Crystal Structure Of 17alpha-Hydroxysteroid Dehydrogenase In Complex With Nadp(H) In A Closed Conformation
GI:126090770 PDB 2HEJ 323 Chain B, Crystal Structure Of 17alpha-Hydroxysteroid Dehydrogenase In Complex With Nadp(H) In A Closed Conformation
GI:126090770 PDB 2P5N 323 Chain A, Crystal Structure Of Mouse 17-alpha Hydroxysteroid Dehydrogenase In Complex With Coenzyme Nadph
GI:126090770 PDB 2P5N 323 Chain B, Crystal Structure Of Mouse 17-alpha Hydroxysteroid Dehydrogenase In Complex With Coenzyme Nadph
GI:126090770 SwissProt Q91WR5.2 323 RecName: Full=Aldo-keto reductase family 1 member C21; AltName: Full=17-alpha-hydroxysteroid dehydrogenase; Short=17-alpha-HSD; AltName: Full=3(or 17)-alpha-hydroxysteroid dehydrogenase; AltName: Full=3-alpha-hydroxysteroid dehydrogenase; AltName: Full=Dihydrodiol dehydrogenase type 1; Short=DD1; AltName: Full=Dihydrodiol dehydrogenase type 3; Short=DD3 [Mus musculus]

Related Sequences to LMP005459 proteins

Reference Database Accession Length Protein Name
GI:126090770 DBBJ BAB32101.1 323 unnamed protein product [Mus musculus]
GI:126090770 GenBank AAH61057.1 323 Aldo-keto reductase family 1, member C21 [Mus musculus]
GI:126090770 GenBank AAW65159.1 323 3(17)alpha-hydroxysteroid dehydrogenase [Mus musculus]
GI:126090770 PDB 3CV6 323 Chain B, The Crystal Structure Of Mouse 17-Alpha Hydroxysteroid Dehydrogenase Gg225.226pp Mutant In Complex With Inhibitor And Cofactor Nadp+.
GI:126090770 PDB 3FJN 323 Chain A, The Crystal Structure Of 17-Alpha Hydroxysteroid Dehydrogenase Y224d Mutant.
GI:126090770 PDB 3FJN 323 Chain B, The Crystal Structure Of 17-Alpha Hydroxysteroid Dehydrogenase Y224d Mutant.