Gene/Proteome Database (LMPD)

LMPD ID
LMP005529
Gene ID
104086
Species
Mus musculus (Mouse)
Gene Name
cytochrome P450, family 27, subfamily a, polypeptide 1
Gene Symbol
Cyp27a1
Synonyms
1300013A03Rik; Cyp27
Alternate Names
sterol 26-hydroxylase, mitochondrial; cytochrome P450 27; cytochrome P450, 27; cytochrome P450, 27a1; sterol 27-hydroxylase; cytochrome P-450C27/25; cholesterol 27 hydroxylase; vitamin D(3) 25-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase
Chromosome
1
Map Location
1 C3|1 38.54 cM
EC Number
1.14.13.15

Proteins

sterol 26-hydroxylase, mitochondrial
Refseq ID NP_077226
Protein GI 30578401
UniProt ID Q9DBG1
mRNA ID NM_024264
Length 533
RefSeq Status PROVISIONAL
MAAWSRTRLRWTLLDPRVVGRGLCPQGARAKATIPAALQAQESTEGPGTGQDRPRLRSPAELPGTGTLQFLFQLFLQGYVLHLPDLQVLNKTKYGPMWTTSFGTYTNVNLASAPLLEQVMRQEGKYPIRDHMDQWKDHRDHKGLTYGIFIAQGEQWYHLRQALKQRLLKPDEAALYTDALNEVISDFITRLDQVRAESESGDQVPDMAHLLYHLALEAITYILFEKRIGCLKPSIPEDTAAFIRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNGWDNIFSFGKKLIDEKVQELKAQLQETGPDGVRVSGYLHFLLTNELLSTQETIGTFPELLLAGVDTTSNTLTWALYHLSKSPEIQEALHKEVTGVVPFGKVPQHKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPNSFQPHRWLRKKEADNPGILHPFGSVPFGYGVRSCLGRRIAELEMQLMLSRLVQKYEIALAPGMGEVKTVSRIVLVPSKKVRLHFLQRQ
transit_peptide: 1..32 inference: non-experimental evidence, no additional details recorded note: Mitochondrion (By similarity); propagated from UniProtKB/Swiss-Prot (Q9DBG1.1) calculated_mol_wt: 3594 peptide sequence: MAAWSRTRLRWTLLDPRVVGRGLCPQGARAKA mat_peptide: 33..533 product: Sterol 26-hydroxylase, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q9DBG1.1) calculated_mol_wt: 57144 peptide sequence: TIPAALQAQESTEGPGTGQDRPRLRSPAELPGTGTLQFLFQLFLQGYVLHLPDLQVLNKTKYGPMWTTSFGTYTNVNLASAPLLEQVMRQEGKYPIRDHMDQWKDHRDHKGLTYGIFIAQGEQWYHLRQALKQRLLKPDEAALYTDALNEVISDFITRLDQVRAESESGDQVPDMAHLLYHLALEAITYILFEKRIGCLKPSIPEDTAAFIRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNGWDNIFSFGKKLIDEKVQELKAQLQETGPDGVRVSGYLHFLLTNELLSTQETIGTFPELLLAGVDTTSNTLTWALYHLSKSPEIQEALHKEVTGVVPFGKVPQHKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPNSFQPHRWLRKKEADNPGILHPFGSVPFGYGVRSCLGRRIAELEMQLMLSRLVQKYEIALAPGMGEVKTVSRIVLVPSKKVRLHFLQRQ

Gene Information

Entrez Gene ID
104086
Gene Name
cytochrome P450, family 27, subfamily a, polypeptide 1
Gene Symbol
Cyp27a1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005743 IDA:MGI C mitochondrial inner membrane
GO:0005739 IDA:MGI C mitochondrion
GO:0047749 IEA:UniProtKB-EC F cholestanetriol 26-monooxygenase activity
GO:0031073 IEA:Ensembl F cholesterol 26-hydroxylase activity
GO:0020037 IEA:InterPro F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0030343 IEA:Ensembl F vitamin D3 25-hydroxylase activity
GO:0008203 IEA:Ensembl P cholesterol metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
mmu03320 PPAR signaling pathway
mmu00120 Primary bile acid biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002401 Cytochrome P450, E-class, group I
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
cytochrome P450, family 27, subfamily a, polypeptide 1
Protein Entry
CP27A_MOUSE
UniProt ID
Q9DBG1
Species
Mouse

Comments

Comment Type Description
Catalytic Activity 5-beta-cholestane-3-alpha,7-alpha,12-alpha- triol + 3 NADPH + 3 O(2) = (25R)-3-alpha,7-alpha,12-alpha- trihydroxy-5-beta-cholestan-26-oate + 3 NADP(+) + 4 H(2)O.
Cofactor Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
Function Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity (By similarity). {ECO:0000250}.
Pathway Hormone biosynthesis; cholecalciferol biosynthesis.
Ptm Acetylation of Lys-125 and Lys-285 is observed in liver mitochondria from fasted mice but not from fed mice. {ECO:0000269|PubMed:23576753}.
Similarity Belongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular Location Mitochondrion membrane {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP005529 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
30578401 RefSeq NP_077226 533 sterol 26-hydroxylase, mitochondrial

Identical Sequences to LMP005529 proteins

Reference Database Accession Length Protein Name
GI:30578401 DBBJ BAB23713.1 533 unnamed protein product [Mus musculus]
GI:30578401 GenBank AAH55028.1 533 Cytochrome P450, family 27, subfamily a, polypeptide 1 [Mus musculus]
GI:30578401 SwissProt Q9DBG1.1 533 RecName: Full=Sterol 26-hydroxylase, mitochondrial; AltName: Full=5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; AltName: Full=Cytochrome P-450C27/25; AltName: Full=Cytochrome P450 27; AltName: Full=Sterol 27-hydroxylase; AltName: Full=Vitamin D(3) 25-hydroxylase; Flags: Precursor [Mus musculus]

Related Sequences to LMP005529 proteins

Reference Database Accession Length Protein Name
GI:30578401 EMBL CAA68822.1 533 vitamin D(3) 25-hydroxylase precursor [Rattus norvegicus]
GI:30578401 GenBank AAH61848.1 533 Cytochrome P450, family 27, subfamily a, polypeptide 1 [Rattus norvegicus]
GI:30578401 GenBank EDL00348.1 533 cytochrome P450, family 27, subfamily a, polypeptide 1 [Mus musculus]
GI:30578401 GenBank EDL75367.1 550 rCG24013, isoform CRA_a [Rattus norvegicus]
GI:30578401 RefSeq NP_849178.2 533 sterol 26-hydroxylase, mitochondrial [Rattus norvegicus]
GI:30578401 SwissProt P17178.1 533 RecName: Full=Sterol 26-hydroxylase, mitochondrial; AltName: Full=5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; AltName: Full=Cytochrome P-450C27/25; AltName: Full=Cytochrome P450 27; AltName: Full=Sterol 27-hydroxylase; AltName: Full=Vitamin D(3) 25-hydroxylase; Flags: Precursor [Rattus norvegicus]