Gene/Proteome Database (LMPD)

LMPD ID
LMP005569
Gene ID
3705
Species
Homo sapiens (Human)
Gene Name
inositol-tetrakisphosphate 1-kinase
Gene Symbol
ITPK1
Synonyms
ITRPK1
Alternate Names
inositol-tetrakisphosphate 1-kinase; ins(1,3,4)P(3) 5/6-kinase; inositol-triphosphate 5/6-kinase; inositol 1,3,4-triphosphate 5/6 kinase; inositol 1,3,4-trisphosphate 5/6 kinase; inositol 1,3,4-trisphosphate 5/6-kinase
Chromosome
14
Map Location
14q31
EC Number
2.7.1.134

Proteins

inositol-tetrakisphosphate 1-kinase isoform a
Refseq ID NP_055031
Protein GI 21359894
UniProt ID Q13572
mRNA ID NM_014216
Length 414
RefSeq Status VALIDATED
MQTFLKGKRVGYWLSEKKIKKLNFQAFAELCRKRGMEVVQLNLSRPIEEQGPLDVIIHKLTDVILEADQNDSQSLELVHRFQEYIDAHPETIVLDPLPAIRTLLDRSKSYELIRKIEAYMEDDRICSPPFMELTSLCGDDTMRLLEKNGLTFPFICKTRVAHGTNSHEMAIVFNQEGLNAIQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTELDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFPGYEGVSEFFTDLLNHIATVLQGQSTAMAATGDVALLRHSKLLAEPAGGLVGERTCSASPGCCGSMMGQDAPWKAEADAGGTAKLPHQRLGCNAGVSPSFQQHCVASLATKASSQ
inositol-tetrakisphosphate 1-kinase isoform a
Refseq ID NP_001136065
Protein GI 217272844
UniProt ID Q13572
mRNA ID NM_001142593
Length 414
RefSeq Status VALIDATED
Protein sequence is identical to GI:21359894 (mRNA isoform)
inositol-tetrakisphosphate 1-kinase isoform b
Refseq ID NP_001136066
Protein GI 217272846
UniProt ID Q13572
mRNA ID NM_001142594
Length 314
RefSeq Status VALIDATED
MQTFLKGKRVGYWLSEKKIKKLNFQAFAELCRKRGMEVVQLNLSRPIEEQGPLDVIIHKLTDVILEADQNDSQSLELVHRFQEYIDAHPETIVLDPLPAIRTLLDRSKSYELIRKIEAYMEDDRICSPPFMELTSLCGDDTMRLLEKNGLTFPFICKTRVAHGTNSHEMAIVFNQEGLNAIQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTELDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFPGDCQVCFIEGWKTD

Gene Information

Entrez Gene ID
3705
Gene Name
inositol-tetrakisphosphate 1-kinase
Gene Symbol
ITPK1
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 TAS:Reactome C cytosol
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0003824 TAS:ProtInc F catalytic activity
GO:0047325 TAS:Reactome F inositol tetrakisphosphate 1-kinase activity
GO:0052825 TAS:Reactome F inositol-1,3,4,5,6-pentakisphosphate 1-phosphatase activity
GO:0052831 TAS:Reactome F inositol-1,3,4,6-tetrakisphosphate 1-phosphatase activity
GO:0052830 TAS:Reactome F inositol-1,3,4,6-tetrakisphosphate 6-phosphatase activity
GO:0052726 TAS:Reactome F inositol-1,3,4-trisphosphate 5-kinase activity
GO:0052725 TAS:Reactome F inositol-1,3,4-trisphosphate 6-kinase activity
GO:0052835 TAS:Reactome F inositol-3,4,6-trisphosphate 1-kinase activity
GO:0016853 IEA:UniProtKB-KW F isomerase activity
GO:0000287 IEA:InterPro F magnesium ion binding
GO:0007596 TAS:Reactome P blood coagulation
GO:0016311 TAS:GOC P dephosphorylation
GO:0043647 TAS:Reactome P inositol phosphate metabolic process
GO:0032957 IEA:InterPro P inositol trisphosphate metabolic process
GO:0007165 TAS:ProtInc P signal transduction
GO:0044281 TAS:Reactome P small molecule metabolic process

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6362 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
PWY-6366 D-myo-inositol (1,4,5,6)-tetrakisphosphate biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_150312 Synthesis of IP3 and IP4 in the cytosol
REACT_150188 Synthesis of pyrophosphates in the cytosol

Domain Information

InterPro Annotations

Accession Description
IPR011761 ATP-grasp fold
IPR008656 Inositol-tetrakisphosphate 1-kinase

UniProt Annotations

Entry Information

Gene Name
inositol-tetrakisphosphate 1-kinase
Protein Entry
ITPK1_HUMAN
UniProt ID
Q13572
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q13572-1; Sequence=Displayed; Name=2; IsoId=Q13572-2; Sequence=VSP_016478, VSP_016479; Note=No experimental confirmation available.;
Biophysicochemical Properties Kinetic parameters: KM=0.3 uM for Ins(1,3,4)P3 ; KM=0.1 uM for Ins(3,4,5,6)P4 ; Vmax=320 pmol/min/ug enzyme with Ins(1,3,4)P3 as substrate ; Vmax=780 pmol/min/ug enzyme enzyme with Ins(3,4,5,6)P4 as substrate ;
Catalytic Activity ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.
Catalytic Activity ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate.
Catalytic Activity ATP + 1D-myo-inositol 3,4,5,6- tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6- pentakisphosphate.
Caution PubMed:11533064 detected some protein kinase activity and ability to phosphorylate transcription factors c-jun/JUN and ATF2. However, PubMed:15762844 showed that it does not have protein kinase activity.
Cofactor Name=Mg(2+); Xref=ChEBI
Function Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain. {ECO
Ptm Acetylation by EP300 and CREBBP destabilizes ITPK1, and down- regulates enzymatic activity. Deacetylated by SIRT1.
Similarity Belongs to the ITPK1 family.
Similarity Contains 1 ATP-grasp domain. {ECO
Subunit Monomer. Interacts with GPS1/COPS1.
Tissue Specificity Expressed in brain > heart > skeletal muscle = kidney = pancreas = liver = placenta > lung. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal cord, occipital lobe, frontal lobe, temporal lobe and putamen.

Identical and Related Proteins

Unique RefSeq proteins for LMP005569 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
21359894 RefSeq NP_055031 414 inositol-tetrakisphosphate 1-kinase isoform a
217272846 RefSeq NP_001136066 314 inositol-tetrakisphosphate 1-kinase isoform b

Identical Sequences to LMP005569 proteins

Reference Database Accession Length Protein Name
GI:217272846 GenBank AAH03622.1 314 ITPK1 protein [Homo sapiens]
GI:217272846 GenBank AAH07428.1 314 ITPK1 protein [Homo sapiens]
GI:217272846 GenBank EAW81511.1 314 inositol 1,3,4-triphosphate 5/6 kinase, isoform CRA_d, partial [Homo sapiens]
GI:21359894 GenBank EAW81512.1 414 inositol 1,3,4-triphosphate 5/6 kinase, isoform CRA_a [Homo sapiens]
GI:21359894 GenBank ABM82360.1 414 inositol 1,3,4-triphosphate 5/6 kinase [synthetic construct]
GI:21359894 GenBank ABM85535.1 414 inositol 1,3,4-triphosphate 5/6 kinase, partial [synthetic construct]
GI:21359894 GenBank AED45336.1 414 Sequence 828 from patent US 7892730
GI:21359894 GenBank AEF64034.1 414 Sequence 330 from patent US 7932031
GI:217272846 GenBank AIC49081.1 314 ITPK1, partial [synthetic construct]
GI:21359894 RefSeq NP_001136065.1 414 inositol-tetrakisphosphate 1-kinase isoform a [Homo sapiens]

Related Sequences to LMP005569 proteins

Reference Database Accession Length Protein Name
GI:21359894 GenBank AAC50483.1 414 inositol 1,3,4-trisphosphate 5/6-kinase [Homo sapiens]
GI:21359894 GenBank AAP36313.1 415 Homo sapiens inositol 1,3,4-triphosphate 5/6 kinase, partial [synthetic construct]
GI:21359894 GenBank AAQ02583.1 415 inositol 1,3,4-triphosphate 5/6 kinase, partial [synthetic construct]
GI:21359894 GenBank AAX43444.1 415 inositol 134-triphosphate 5/6 kinase, partial [synthetic construct]
GI:21359894 GenBank AAX43445.1 415 inositol 134-triphosphate 5/6 kinase, partial [synthetic construct]
GI:21359894 GenBank ACM81364.1 414 Sequence 6862 from patent US 6812339
GI:217272846 GenBank AED45336.1 414 Sequence 828 from patent US 7892730
GI:217272846 GenBank AEF64034.1 414 Sequence 330 from patent US 7932031
GI:217272846 RefSeq NP_055031.2 414 inositol-tetrakisphosphate 1-kinase isoform a [Homo sapiens]
GI:217272846 RefSeq XP_005562118.1 368 PREDICTED: inositol-tetrakisphosphate 1-kinase isoform X2 [Macaca fascicularis]
GI:217272846 RefSeq XP_008956466.1 322 PREDICTED: inositol-tetrakisphosphate 1-kinase isoform X2 [Pan paniscus]
GI:217272846 SwissProt Q13572.2 414 RecName: Full=Inositol-tetrakisphosphate 1-kinase; AltName: Full=Inositol 1,3,4-trisphosphate 5/6-kinase; Short=Inositol-triphosphate 5/6-kinase; Short=Ins(1,3,4)P(3) 5/6-kinase [Homo sapiens]