Gene/Proteome Database (LMPD)
Proteins
| acetyl-CoA carboxylase 2 precursor | |
|---|---|
| Refseq ID | NP_598665 |
| Protein GI | 157042798 |
| UniProt ID | Q9ESZ3 |
| mRNA ID | NM_133904 |
| Length | 2448 |
| RefSeq Status | VALIDATED |
| MVLLLFLTCLVFSCLTFSWLKIWGKMTDSKPLTNSKVEANLLSSEESLSASELSGEQLQEHGDHSCLSYRGPRDASQQRNSLPSSCQRPPRNPLSSNDTWPSPELQTNWTAAPGPEVPDANGLSFPARPPSQRTVSPSREDRKQAHIKRQLMTSFILGSLDDNSSDEDPSAGSFQNSSRKSSRASLGTLSQEAALNTSDPESHAPTMRPSMSGLHLVKRGREHKKLDLHRDFTVASPAEFVTRFGGNRVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADQYVPVPGGPNNNNYANVELIIDIAKRIPVQAVWAGWGHASENPKLPELLCKHEIAFLGPPSEAMWALGDKIASTIVAQTLQIPTLPWSGSGLTVEWTEDSRHQGKCISVPEDVYEQGCVKDVDEGLQAAEKIGFPLMIKASEGGGGKGIRKAESAEDFPMLFRQVQSEIPGSPIFLMKLAQNARHLEVQVLADQYGNAVSLFGRDCSIQRRHQKIIEEAPATIAAPAVFEFMEQCAVLLAKMVGYVSAGTVEYLYSQDGSFHFLELNPRLQVEHPCTEMIADVNLPAAQLQIAMGVPLHRLKDIRLLYGESPWGVTPIPFETPLSPPIARGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLVNLLETESFQNNDIDTGWLDHLIAQRVQAEKPDIMLGVVCGALNVADAMFRTCMTEFLHSLERGQVLPADSLLNIVDVELIYGGIKYALKVARQSLTMFVLIMNGCHIEIDAHRLNDGGLLLSYNGSSYTTYMKEEVDSYRITIGNKTCVFEKENDPTVLRSPSAGKLMQYTVEDGDHVEAGSSYAEMEVMKMIMTLNVQESGRVKYIKRPGVILEAGCVVARLELDDPSKVHAAQPFTGELPAQQTLPILGEKLHQVFHGVLENLTNVMSGYCLPEPFFSMKLKDWVQKLMMTLRHPSLPLLELQEIMTSVAGRIPAPVEKAVRRVMAQYASNITSVLCQFPSQQIATILDCHAATLQRKADREVFFMNTQSIVQLVQRYRSGTRGYMKAVVLDLLRKYLNVEHHFQQAHYDKCVINLREQFKPDMTQVLDCIFSHSQVAKKNQLVTMLIDELCGPDPTLSDELTSILCELTQLSRSEHCKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCPENLKKLILSETTIFDVLPTFFYHENKVVCMASLEVYVRRGYIAYELNSLQHRELPDGTCVVEFQFMLPSSHPNRMAVPISVSNPDLLRHSTELFMDSGFSPLCQRMGAMVAFRRFEEFTRNFDEVISCFANVQTDTLLFSKACTSLYSEEDSKSLREEPIHILNVAIQCADHMEDEALVPVFRAFVQSKKHILVDYGLRRITFLVAQEREFPKFFTFRARDEFAEDRIYRHLEPALAFQLELSRMRNFDLTAVPCANHKMHLYLGAAKVKEGLEVTDHRFFIRAIIRHSDLITKEASFEYLQNEGERLLLEAMDELEVAFNNTSVRTDCNHIFLNFVPTVIMDPLKIEESVRDMVMRYGSRLWKLRVLQAEVKINIRQTTSDSAIPIRLFITNESGYYLDISLYREVTDSRSGNIMFHSFGNKQGSLHGMLINTPYVTKDLLQAKRFQAQSLGTTYVYDFPEMFRQALFKLWGSPEKYPKDILTYTELVLDSQGQLVEMNRLPGCNEVGMVAFKMRFKTPEYPEGRDAVVIGNDITFQIGSFGIGEDFLYLRASEMARTEGIPQIYLAANSGARMGLAEEIKQIFQVAWVDPEDPHKGFRYLYLTPQDYTQISSQNSVHCKHIEDEGESRYVIVDVIGKDANLGVENLRGSGMIAGEASLAYEKTVTISMVTCRALGIGAYLVRLGQRVIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHTNGVSHVTVPDDFEGVCTILEWLSFIPKDNRSPVPITTPSDPIDREIEFTPTKAPYDPRWMLAGRPHPTLKGTWQSGFFDHGSFKEIMAPWAQTVVTGRARLGGIPVGVIAVETRTVEVAVPADPANLDSEAKIIQQAGQVWFPDSAYKTAQVIRDFNKERLPLMIFANWRGFSGGMKDMYEQMLKFGAYIVDGLRLYEQPILIYIPPCAELRGGSWVVLDSTINPLCIEMYADKESRGGVLEPEGTVEIKFRKKDLVKTIRRIDPVCKKLVGQLGKAQLPDKDRKELEGQLKAREELLLPIYHQVAVQFADLHDTPGHMLEKGIISDVLEWKTARTFFYWRLRRLLLEAQVKQEILRASPELNHEHTQSMLRRWFVETEGAVKAYLWDSNQVVVQWLEQHWSAKDGLRSTIRENINYLKRDSVLKTIQSLVQEHPEVIMDCVAYLSQHLTPAERIQVAQLLSTTESPASS | |
| sig_peptide: 1..16 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 1816 peptide sequence: MVLLLFLTCLVFSCLT | |
Gene Information
Entrez Gene ID
Gene Name
acetyl-Coenzyme A carboxylase beta
Gene Symbol
Species
Mus musculus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005739 | IDA:UniProtKB | C | mitochondrion |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0003989 | IDA:MGI | F | acetyl-CoA carboxylase activity |
| GO:0009374 | IEA:Ensembl | F | biotin binding |
| GO:0004075 | IEA:InterPro | F | biotin carboxylase activity |
| GO:0046872 | IEA:InterPro | F | metal ion binding |
| GO:0006084 | IEA:Ensembl | P | acetyl-CoA metabolic process |
| GO:0006633 | IMP:MGI | P | fatty acid biosynthetic process |
| GO:2001295 | IMP:UniProtKB | P | malonyl-CoA biosynthetic process |
| GO:0046322 | IMP:UniProtKB | P | negative regulation of fatty acid oxidation |
| GO:0060421 | IMP:UniProtKB | P | positive regulation of heart growth |
| GO:0010884 | IMP:UniProtKB | P | positive regulation of lipid storage |
| GO:0051289 | IEA:Ensembl | P | protein homotetramerization |
| GO:0042493 | IEA:Ensembl | P | response to drug |
| GO:0014070 | IEA:Ensembl | P | response to organic cyclic compound |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR011761 | ATP-grasp fold |
| IPR013815 | ATP-grasp fold, subdomain 1 |
| IPR013816 | ATP-grasp fold, subdomain 2 |
| IPR013537 | Acetyl-CoA carboxylase, central domain |
| IPR011763 | Acetyl-coenzyme A carboxyltransferase, C-terminal |
| IPR011762 | Acetyl-coenzyme A carboxyltransferase, N-terminal |
| IPR005482 | Biotin carboxylase, C-terminal |
| IPR011764 | Biotin carboxylation domain |
| IPR000089 | Biotin/lipoyl attachment |
| IPR005481 | Carbamoyl-phosphate synthase, large subunit, N-terminal |
| IPR005479 | Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain |
| IPR000022 | Carboxyl transferase |
| IPR029045 | ClpP/crotonase-like domain |
| IPR016185 | Pre-ATP-grasp domain |
| IPR011054 | Rudiment single hybrid motif |
| IPR011053 | Single hybrid motif |
UniProt Annotations
Entry Information
Gene Name
acetyl-Coenzyme A carboxylase beta
Protein Entry
E9Q4Z2_MOUSE
UniProt ID
Species
Mouse
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA. |
| Catalytic Activity | ATP + biotin-[carboxyl-carrier-protein] + CO(2) = ADP + phosphate + carboxy-biotin-[carboxyl-carrier- protein]. |
| Cofactor | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ; Note=Binds 2 manganese ions per subunit. {ECO:0000255|PROSITE- ProRule:PRU00409}; |
| Cofactor | Name=biotin; Xref=ChEBI:CHEBI:57586; Evidence= ; Note=Biotin. ; |
| Disruption Phenotype | Normal morphology, fertility, growth rate and lifespan but higher than normal food consumption and fatty acid oxidation rate and decreased fat content in adipose tissue and liver (PubMed:11283375). A high-fat/high-carbohydrate diet results in maintenance of normal insulin and glucose levels with less weight gain and less fat accumulation than wild-type mice (PubMed:12920182). Elevated levels of Ucp2 in adipose tissue and heart but not in skeletal muscle or liver, and elevated levels of Ucp3 in skeletal muscle but not in heart or brown adipose tissue (PubMed:12920182). Significant decrease in body weight, weight of epidydimal fat pads and levels of hepatic triglycerides under a range of dietary conditions including normal chow diet, fasting and refeeding a fat-free high-carbohydrate diet, and a high- fat/high-carbohydrate diet (PubMed:22362781). Up-regulation of lipogenic enzymes under de novo lipogenic conditions but reduced fat accumulation in liver (PubMed:22362781). Primary cultured adipocytes show increased fatty acid and glucose oxidation rates and increased lipolysis (PubMed:15677334). Reduced heart size, reduced Mlycd and malonyl-CoA levels in mutant hearts, reduced myocardial triglyceride levels, higher myocardial oleate and glucose oxidation rates, reduced levels of Ppara and reduced activation of Mtor (PubMed:18487439, PubMed:22730442). However, it has also been reported that mutants show no differences in body weight, food intake, body composition or glucose homeostasis as compared with controls fed on chow or a high-fat diet (PubMed:20368432). {ECO:0000269|PubMed:11283375, ECO:0000269|PubMed:12920182, ECO:0000269|PubMed:15677334, ECO:0000269|PubMed:18487439, ECO:0000269|PubMed:20368432, ECO:0000269|PubMed:22362781, ECO:0000269|PubMed:22730442}. |
| Enzyme Regulation | Activity is increased by oligomerization. Activated by citrate. Citrate and MID1IP1 promote oligomerization. Inhibited by malonyl-CoA. |
| Function | Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA (By similarity). Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase (By similarity). Involved in inhibition of fatty acid and glucose oxidation and enhancement of fat storage (PubMed:11283375, PubMed:12920182, PubMed:15677334, PubMed:18487439, PubMed:22362781). May play a role in regulation of mitochondrial fatty acid oxidation through malonyl-CoA- dependent inhibition of carnitine palmitoyltransferase 1 (PubMed:10677481). {ECO:0000250|UniProtKB:O00763, ECO:0000269|PubMed:11283375, ECO:0000269|PubMed:12920182, ECO:0000269|PubMed:15677334, ECO:0000269|PubMed:18487439, ECO:0000269|PubMed:22362781, ECO:0000303|PubMed:10677481}. |
| Pathway | Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. |
| Ptm | Phosphorylated by AMPK, leading to inactivation of the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis. |
| Similarity | Contains 1 ATP-grasp domain. {ECO:0000255|PROSITE- ProRule:PRU00409}. |
| Similarity | Contains 1 biotin carboxylation domain. |
| Similarity | Contains 1 biotinyl-binding domain. |
| Similarity | Contains 1 carboxyltransferase domain. |
| Subcellular Location | Mitochondrion . Nucleus . Endomembrane system . Note=May associate with membranes. |
| Subunit | Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity. |
Identical and Related Proteins
Unique RefSeq proteins for LMP005741 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 157042798 | RefSeq | NP_598665 | 2448 | acetyl-CoA carboxylase 2 precursor |
Identical Sequences to LMP005741 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:157042798 | RefSeq | XP_006530176.1 | 2448 | PREDICTED: acetyl-Coenzyme A carboxylase beta isoform X3 [Mus musculus] |
| GI:157042798 | SwissProt | E9Q4Z2.1 | 2448 | RecName: Full=Acetyl-CoA carboxylase 2; AltName: Full=ACC-beta; Includes: RecName: Full=Biotin carboxylase; Flags: Precursor [Mus musculus] |
Related Sequences to LMP005741 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:157042798 | GenBank | AAS13686.1 | 2448 | acetyl-CoA carboxylase 2 [Mus musculus] |
| GI:157042798 | GenBank | ABF48724.1 | 2455 | acetyl-Coenzyme A carboxylase 2 [Rattus norvegicus] |
| GI:157042798 | GenBank | ABT02350.1 | 2455 | Sequence 16 from patent US 7211423 |
| GI:157042798 | GenBank | AGM84071.1 | 2448 | Sequence 16 from patent US 8389207 |
| GI:157042798 | RefSeq | XP_006530174.1 | 2469 | PREDICTED: acetyl-Coenzyme A carboxylase beta isoform X1 [Mus musculus] |
| GI:157042798 | RefSeq | XP_006530175.1 | 2469 | PREDICTED: acetyl-Coenzyme A carboxylase beta isoform X2 [Mus musculus] |