Gene/Proteome Database (LMPD)

LMPD ID
LMP005936
Gene ID
101540
Species
Mus musculus (Mouse)
Gene Name
protein kinase D2
Gene Symbol
Prkd2
Synonyms
AI325941; PKD2
Alternate Names
serine/threonine-protein kinase D2; nPKC-D2
Chromosome
7
Map Location
7 A2|7
EC Number
2.7.11.13

Proteins

serine/threonine-protein kinase D2
Refseq ID NP_849231
Protein GI 30725754
UniProt ID Q8BZ03
mRNA ID NM_178900
Length 875
RefSeq Status VALIDATED
MAAAPSHPAGLPGSPGPGSPPPPGGLDLQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSAADIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGSSESLPCTAEELSRSTTDLLPRRPPSSSSSSSSSSFYTGRPIELDKMLMSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEAADYSEADKSSISDELEDSGVIPGSHSESALHASEEEEGEGHKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILAVEPAQNFSLVPPGTNPHCFEIITANVTYFVGETPGGAPGGPSGQGTEAVRGWETAIRQALMPVILQDAPSAPGHTPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISSGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWDQFVAERHGTPAEGDLGGACLPQDHEMQGLAERISIL
serine/threonine-protein kinase D2
Refseq ID NP_001239387
Protein GI 356995870
UniProt ID Q8BZ03
mRNA ID NM_001252458
Length 875
RefSeq Status VALIDATED
Protein sequence is identical to GI:30725754 (mRNA isoform)

Gene Information

Entrez Gene ID
101540
Gene Name
protein kinase D2
Gene Symbol
Prkd2
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0005634 IEA:Ensembl C nucleus
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0004697 IEA:UniProtKB-EC F protein kinase C activity
GO:0050852 IEA:Ensembl P T cell receptor signaling pathway
GO:0001525 IEA:UniProtKB-KW P angiogenesis
GO:0007155 IEA:UniProtKB-KW P cell adhesion
GO:0008219 IEA:Ensembl P cell death
GO:0035556 IEA:Ensembl P intracellular signal transduction
GO:0018105 IEA:Ensembl P peptidyl-serine phosphorylation
GO:0032793 IEA:Ensembl P positive regulation of CREB transcription factor activity
GO:2000573 IDA:UniProtKB P positive regulation of DNA biosynthetic process
GO:0070374 IDA:UniProtKB P positive regulation of ERK1 and ERK2 cascade
GO:0051092 ISS:UniProtKB P positive regulation of NF-kappaB transcription factor activity
GO:0050862 IMP:UniProtKB P positive regulation of T cell receptor signaling pathway
GO:0045766 ISS:UniProtKB P positive regulation of angiogenesis
GO:0043536 IEA:Ensembl P positive regulation of blood vessel endothelial cell migration
GO:0045785 ISS:UniProtKB P positive regulation of cell adhesion
GO:0038033 IEA:Ensembl P positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
GO:0010595 ISS:UniProtKB P positive regulation of endothelial cell migration
GO:0001938 ISS:UniProtKB P positive regulation of endothelial cell proliferation
GO:0045743 ISS:UniProtKB P positive regulation of fibroblast growth factor receptor signaling pathway
GO:1901727 IEA:Ensembl P positive regulation of histone deacetylase activity
GO:0032743 IMP:UniProtKB P positive regulation of interleukin-2 production
GO:0032757 ISS:UniProtKB P positive regulation of interleukin-8 production
GO:0033138 IEA:Ensembl P positive regulation of peptidyl-serine phosphorylation
GO:0051091 ISS:UniProtKB P positive regulation of sequence-specific DNA binding transcription factor activity
GO:0045944 IEA:Ensembl P positive regulation of transcription from RNA polymerase II promoter
GO:0030949 ISS:UniProtKB P positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0046777 IEA:Ensembl P protein autophosphorylation

KEGG Pathway Links

KEGG Pathway ID Description
ko04015 Rap1 signaling pathway
mmu04015 Rap1 signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR020454 Diacylglycerol/phorbol-ester binding
IPR001849 Pleckstrin homology domain
IPR011993 Pleckstrin homology-like domain
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
IPR000719 Protein kinase domain
IPR017441 Protein kinase, ATP binding site
IPR011009 Protein kinase-like domain
IPR015727 Protein_Kinase_C_mu-related
IPR008271 Serine/threonine-protein kinase, active site
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
protein kinase D2
Protein Entry
KPCD2_MOUSE
UniProt ID
Q8BZ03
Species
Mouse

Comments

Comment Type Description
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Enzyme Regulation Activated by DAG and phorbol esters. Phorbol- ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-711 and phosphorylation of Ser-707 by PKC relieves auto-inhibition by the PH domain (By similarity). {ECO:0000250}.
Function Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress- induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-439 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF- kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2- induced monocyte adhesion to endothelial cells. {ECO:0000269|PubMed:17226786, ECO:0000269|PubMed:20819079}.
Ptm Phosphorylation of Ser-873 correlates with the activation status of the kinase. Ser-707 is probably phosphorylated by PKC. Phosphorylation at Ser-244 by CSNK1D and CSNK1E promotes nuclear localization and substrate targeting. Phosphorylation at Ser-244, Ser-707 and Ser-711 is required for nuclear localization. Phosphorylated at Tyr-438 by ABL1 in response to oxidative stress (By similarity). {ECO:0000250}.
Similarity Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. {ECO:0000305}.
Similarity Contains 1 PH domain. {ECO:0000255|PROSITE- ProRule:PRU00145}.
Similarity Contains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}.
Similarity Contains 2 phorbol-ester/DAG-type zinc fingers. {ECO:0000255|PROSITE-ProRule:PRU00226}.
Subcellular Location Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Golgi apparatus, trans-Golgi network {ECO:0000250}. Note=Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import (By similarity). {ECO:0000250}.
Subunit Interacts (via C-terminus) with LCK. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP005936 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
30725754 RefSeq NP_849231 875 serine/threonine-protein kinase D2

Identical Sequences to LMP005936 proteins

Reference Database Accession Length Protein Name
GI:30725754 DBBJ BAC29677.1 875 unnamed protein product [Mus musculus]
GI:30725754 GenBank AAH95949.1 875 Prkd2 protein [Mus musculus]
GI:30725754 GenBank AAH96444.1 875 Prkd2 protein [Mus musculus]
GI:30725754 RefSeq NP_001239387.1 875 serine/threonine-protein kinase D2 [Mus musculus]
GI:30725754 SwissProt Q8BZ03.1 875 RecName: Full=Serine/threonine-protein kinase D2; AltName: Full=nPKC-D2 [Mus musculus]

Related Sequences to LMP005936 proteins

Reference Database Accession Length Protein Name
GI:30725754 DBBJ BAE32326.1 875 unnamed protein product [Mus musculus]
GI:30725754 GenBank AAH83592.1 875 Protein kinase D2 [Rattus norvegicus]
GI:30725754 GenBank EDL42075.1 912 protein kinase D2, isoform CRA_a, partial [Mus musculus]
GI:30725754 RefSeq NP_001013917.1 875 serine/threonine-protein kinase D2 [Rattus norvegicus]
GI:30725754 RefSeq XP_008757127.1 875 PREDICTED: serine/threonine-protein kinase D2 isoform X1 [Rattus norvegicus]
GI:30725754 SwissProt Q5XIS9.1 875 RecName: Full=Serine/threonine-protein kinase D2; AltName: Full=nPKC-D2 [Rattus norvegicus]