Gene/Proteome Database (LMPD)
LMPD ID
LMP006123
Gene ID
Species
Mus musculus (Mouse)
Gene Name
O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Gene Symbol
Synonyms
1110038P24Rik; 4831420N21Rik; AI115525; Ogtl
Alternate Names
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit
Chromosome
X
Map Location
X|X D
EC Number
2.4.1.255
Proteins
| UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 1 | |
|---|---|
| Refseq ID | NP_631883 |
| Protein GI | 46909607 |
| UniProt ID | Q8CGY8 |
| mRNA ID | NM_139144 |
| Length | 1046 |
| RefSeq Status | VALIDATED |
| MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVAEQLEKNRLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNPDSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKSRQEYEDIAVKLGTDLEYLKKIRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA | |
| UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2 | |
|---|---|
| Refseq ID | NP_001277464 |
| Protein GI | 594542444 |
| UniProt ID | Q8CGY8 |
| mRNA ID | NM_001290535 |
| Length | 1036 |
| RefSeq Status | VALIDATED |
| MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVAEQLEKNRLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNPDSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKSRQEYEDIAVKLGTDLEYLKKIRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA | |
Gene Information
Entrez Gene ID
Gene Name
O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Gene Symbol
Species
Mus musculus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0070688 | IEA:Ensembl | C | MLL5-L complex |
| GO:0005737 | TAS:MGI | C | cytoplasm |
| GO:0005829 | ISS:UniProtKB | C | cytosol |
| GO:0000123 | ISS:UniProtKB | C | histone acetyltransferase complex |
| GO:0005622 | TAS:MGI | C | intracellular |
| GO:0005815 | IEA:Ensembl | C | microtubule organizing center |
| GO:0005634 | ISS:UniProtKB | C | nucleus |
| GO:0005886 | ISS:UniProtKB | C | plasma membrane |
| GO:0008080 | TAS:MGI | F | N-acetyltransferase activity |
| GO:0003824 | ISS:MGI | F | catalytic activity |
| GO:0008047 | IEA:Ensembl | F | enzyme activator activity |
| GO:0046972 | IEA:Ensembl | F | histone acetyltransferase activity (H4-K16 specific) |
| GO:0043995 | IEA:Ensembl | F | histone acetyltransferase activity (H4-K5 specific) |
| GO:0043996 | IEA:Ensembl | F | histone acetyltransferase activity (H4-K8 specific) |
| GO:0005547 | ISS:UniProtKB | F | phosphatidylinositol-3,4,5-trisphosphate binding |
| GO:0016262 | ISS:UniProtKB | F | protein N-acetylglucosaminyltransferase activity |
| GO:0097363 | IMP:UniProtKB | F | protein O-GlcNAc transferase activity |
| GO:0006915 | ISS:UniProtKB | P | apoptotic process |
| GO:0071300 | IEA:Ensembl | P | cellular response to retinoic acid |
| GO:0032922 | IMP:UniProtKB | P | circadian regulation of gene expression |
| GO:0080182 | IEA:Ensembl | P | histone H3-K4 trimethylation |
| GO:0043984 | ISS:UniProtKB | P | histone H4-K16 acetylation |
| GO:0043981 | ISS:UniProtKB | P | histone H4-K5 acetylation |
| GO:0043982 | ISS:UniProtKB | P | histone H4-K8 acetylation |
| GO:0031397 | IDA:UniProtKB | P | negative regulation of protein ubiquitination |
| GO:0048015 | ISS:UniProtKB | P | phosphatidylinositol-mediated signaling |
| GO:0030854 | IEA:Ensembl | P | positive regulation of granulocyte differentiation |
| GO:0061087 | ISS:UniProtKB | P | positive regulation of histone H3-K27 methylation |
| GO:0051571 | IEA:Ensembl | P | positive regulation of histone H3-K4 methylation |
| GO:0045862 | ISS:UniProtKB | P | positive regulation of proteolysis |
| GO:0045944 | IEA:Ensembl | P | positive regulation of transcription from RNA polymerase II promoter |
| GO:0006493 | ISS:UniProtKB | P | protein O-linked glycosylation |
| GO:0035020 | IEA:Ensembl | P | regulation of Rac protein signal transduction |
| GO:0090526 | IMP:UniProtKB | P | regulation of gluconeogenesis involved in cellular glucose homeostasis |
| GO:0006110 | ISS:UniProtKB | P | regulation of glycolytic process |
| GO:0046626 | IEA:Ensembl | P | regulation of insulin receptor signaling pathway |
| GO:0032868 | IEA:Ensembl | P | response to insulin |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko00514 | Other types of O-glycan biosynthesis |
| mmu00514 | Other types of O-glycan biosynthesis |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 5894128 | HATs acetylate histones |
Domain Information
UniProt Annotations
Entry Information
Gene Name
O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Protein Entry
OGT1_MOUSE
UniProt ID
Species
Mouse
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q8CGY8-1; Sequence=Displayed; Name=2; IsoId=Q8CGY8-2; Sequence=VSP_024844; |
| Catalytic Activity | UDP-N-acetyl-D-glucosamine + [protein]-L- serine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine. |
| Catalytic Activity | UDP-N-acetyl-D-glucosamine + [protein]-L- threonine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L- threonine. |
| Domain | The TPR repeat domain is required for substrate binding and oligomerization. {ECO:0000250}. |
| Function | Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta- linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O- GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (By similarity). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2. {ECO:0000250, ECO:0000269|PubMed:23337503, ECO:0000269|PubMed:23395176}. |
| Induction | Expression in the liver oscillates in a circadian manner. {ECO:0000269|PubMed:23395176}. |
| Pathway | Protein modification; protein glycosylation. |
| Ptm | Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity. {ECO:0000269|PubMed:23395175}. |
| Ptm | Ubiquitinated, leading to its proteasomal degradation. {ECO:0000250}. |
| Similarity | Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily. {ECO:0000305}. |
| Similarity | Contains 13 TPR repeats. {ECO:0000255|PROSITE- ProRule:PRU00339}. |
| Subcellular Location | Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1. After insulin induction, translocated from the nucleus to the cell membrane via phophatidylinisotide binding. Colocalizes with AKT1 at the plasma membrane (By similarity). {ECO:0000250}. |
| Subunit | Heterotrimer; consists of one 78 kDa subunit and two 110 kDa subunits dimerized via TPR repeats 6 and 7 (By similarity). Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, HCFC1, PPP1CC and ACTB. Component of a THAP1/THAP3-HCFC1-OGT complex. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts directly with HCFC1; the interaction O-glycosylates HCFC1, regulates its proteolytic processing and transcriptional activity and, in turn, stabilizes OGT in the nucleus. Interacts (via TPRs 1-6) with SIN3A; the interaction mediates transcriptional repression in parallel with histone deacetylase (By similarity). Interacts (via TPR 5-6) with TET1, TET2 and TET3. Interacts (via TPR repeats 6 and 7) with ATXN10. Interacts with ARNTL/BMAL1. {ECO:0000250, ECO:0000269|PubMed:16182253, ECO:0000269|PubMed:23337503, ECO:0000269|PubMed:23352454}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP006123 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 46909607 | RefSeq | NP_631883 | 1046 | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 1 |
| 594542444 | RefSeq | NP_001277464 | 1036 | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2 |
Identical Sequences to LMP006123 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:46909607 | GenBank | AAH57319.1 | 1046 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) [Mus musculus] |
| GI:594542444 | GenBank | EDL14137.1 | 1036 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_a [Mus musculus] |
| GI:46909607 | GenBank | EDL14138.1 | 1046 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_b [Mus musculus] |
| GI:46909607 | GenBank | AFG79915.1 | 1046 | Sequence 174 from patent US 8137928 |
| GI:46909607 | RefSeq | XP_006527799.1 | 1046 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Mus musculus] |
| GI:46909607 | SwissProt | Q8CGY8.2 | 1046 | RecName: Full=UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; AltName: Full=O-GlcNAc transferase subunit p110; AltName: Full=O-linked N-acetylglucosamine transferase 110 kDa subunit; Short=OGT [Mus musculus] |
Related Sequences to LMP006123 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:46909607 | GenBank | AAK39123.1 | 1046 | UDP-N-acetylglucosaminyltransferase [Mus musculus] |
| GI:46909607 | GenBank | EDL95886.1 | 1046 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_a [Rattus norvegicus] |
| GI:594542444 | GenBank | EDL95887.1 | 1036 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_b [Rattus norvegicus] |
| GI:594542444 | GenBank | EDL95888.1 | 1036 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_b [Rattus norvegicus] |
| GI:594542444 | RefSeq | NP_058803.2 | 1036 | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit [Rattus norvegicus] |
| GI:46909607 | RefSeq | XP_005358617.1 | 1046 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Microtus ochrogaster] |
| GI:594542444 | RefSeq | XP_005358618.1 | 1036 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Microtus ochrogaster] |
| GI:46909607 | RefSeq | XP_006257118.1 | 1046 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Rattus norvegicus] |
| GI:46909607 | RefSeq | XP_006257119.1 | 1046 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Rattus norvegicus] |
| GI:594542444 | RefSeq | XP_006257120.1 | 1036 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Rattus norvegicus] |
| GI:46909607 | RefSeq | XP_007632584.1 | 1046 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Cricetulus griseus] |
| GI:594542444 | RefSeq | XP_007632585.1 | 1036 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Cricetulus griseus] |