Gene/Proteome Database (LMPD)

LMPD ID
LMP006280
Gene ID
253558
Species
Homo sapiens (Human)
Gene Name
lysocardiolipin acyltransferase 1
Gene Symbol
LCLAT1
Synonyms
1AGPAT8; AGPAT8; ALCAT1; HSRG1849; LYCAT; UNQ1849
Alternate Names
lysocardiolipin acyltransferase 1; 1-AGPAT 8; 1-AGP acyltransferase 8; acyl-CoA:lysocardiolipin acyltransferase 1; 1-acylglycerol-3-phosphate O-acyltransferase 8
Chromosome
2
Map Location
2p23.1
EC Number
2.3.1.-

Proteins

lysocardiolipin acyltransferase 1 isoform 1
Refseq ID NP_872357
Protein GI 42558246
UniProt ID Q6UWP7
mRNA ID NM_182551
Length 414
RefSeq Status VALIDATED
MHSRGREIVVLLNPWSINEAVSSYCTYFIKQDSKSFGIMVSWKGIYFILTLFWGSFFGSIFMLSPFLPLMFVNPSWYRWINNRLVATWLTLPVALLETMFGVKVIITGDAFVPGERSVIIMNHRTRMDWMFLWNCLMRYSYLRLEKICLKASLKGVPGFGWAMQAAAYIFIHRKWKDDKSHFEDMIDYFCDIHEPLQLLIFPEGTDLTENSKSRSNAFAEKNGLQKYEYVLHPRTTGFTFVVDRLREGKNLDAVHDITVAYPHNIPQSEKHLLQGDFPREIHFHVHRYPIDTLPTSKEDLQLWCHKRWEEKEERLRSFYQGEKNFYFTGQSVIPPCKSELRVLVVKLLSILYWTLFSPAMCLLIYLYSLVKWYFIITIVIFVLQERIFGGLEIIELACYRLLHKQPHLNSKKNE
lysocardiolipin acyltransferase 1 isoform 2
Refseq ID NP_001002257
Protein GI 50659059
UniProt ID Q6UWP7
mRNA ID NM_001002257
Length 376
RefSeq Status VALIDATED
MVSWKGIYFILTLFWGSFFGSIFMLSPFLPLMFVNPSWYRWINNRLVATWLTLPVALLETMFGVKVIITGDAFVPGERSVIIMNHRTRMDWMFLWNCLMRYSYLRLEKICLKASLKGVPGFGWAMQAAAYIFIHRKWKDDKSHFEDMIDYFCDIHEPLQLLIFPEGTDLTENSKSRSNAFAEKNGLQKYEYVLHPRTTGFTFVVDRLREGKNLDAVHDITVAYPHNIPQSEKHLLQGDFPREIHFHVHRYPIDTLPTSKEDLQLWCHKRWEEKEERLRSFYQGEKNFYFTGQSVIPPCKSELRVLVVKLLSILYWTLFSPAMCLLIYLYSLVKWYFIITIVIFVLQERIFGGLEIIELACYRLLHKQPHLNSKKNE

Gene Information

Entrez Gene ID
253558
Gene Name
lysocardiolipin acyltransferase 1
Gene Symbol
LCLAT1
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005789 TAS:Reactome C endoplasmic reticulum membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:UniProtKB C membrane
GO:0003841 IEA:UniProtKB-EC F 1-acylglycerol-3-phosphate O-acyltransferase activity
GO:0035965 TAS:Reactome P cardiolipin acyl-chain remodeling
GO:0016024 IEA:UniProtKB-UniPathway P CDP-diacylglycerol biosynthetic process
GO:0044255 TAS:Reactome P cellular lipid metabolic process
GO:0046474 TAS:Reactome P glycerophospholipid biosynthetic process
GO:0007275 IEA:UniProtKB-KW P multicellular organismal development
GO:0006654 TAS:Reactome P phosphatidic acid biosynthetic process
GO:0006644 TAS:Reactome P phospholipid metabolic process
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0019432 TAS:Reactome P triglyceride biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
hsa00564 Glycerophospholipid metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_121006 Acyl chain remodeling of CL
REACT_120906 Synthesis of PA

Domain Information

InterPro Annotations

Accession Description
IPR002123 Phospholipid/glycerol acyltransferase

UniProt Annotations

Entry Information

Gene Name
lysocardiolipin acyltransferase 1
Protein Entry
LCLT1_HUMAN
UniProt ID
Q6UWP7
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q6UWP7-1; Sequence=Displayed; Name=2; IsoId=Q6UWP7-2; Sequence=VSP_026181, VSP_026182; Note=No experimental confirmation available.; Name=3; IsoId=Q6UWP7-3; Sequence=VSP_044307;
Biophysicochemical Properties Kinetic parameters: KM=39 uM for arachidonoyl-CoA (20:4) for LPIAT activity ; KM=289 uM for oleoyl-CoA (18:1) for LPIAT activity ; KM=134 uM for linoleoyl-CoA (18:2) for LPIAT activity ; KM=34 uM for stearoyl-CoA (18:0) for LPIAT activity ; KM=53 uM for palmitoyl-CoA (16:0) for LPIAT activity ; KM=54 uM for oleoyl-CoA (18:1) for LPGAT activity ; KM=36 uM for linoleoyl-CoA (18:2) for LPGAT activity ; KM=70 uM for stearoyl-CoA (18:0) for LPGAT activity ; KM=34 uM for palmitoyl-CoA (16:0) for LPGAT activity ; Vmax=3230 nmol/min/mg enzyme toward arachidonoyl-CoA (20:4) for LPIAT activity ; Vmax=7489 nmol/min/mg enzyme toward oleoyl-CoA (18:1) for LPIAT activity ; Vmax=4696 nmol/min/mg enzyme toward linoleoyl-CoA (18:2) for LPIAT activity ; Vmax=1078 nmol/min/mg enzyme toward stearoyl-CoA (18:0) for LPIAT activity ; Vmax=11203 nmol/min/mg enzyme toward palmitoyl-CoA (16:0) for LPIAT activity ; Vmax=5514 nmol/min/mg enzyme toward oleoyl-CoA (18:1) for LPGAT activity ; Vmax=1358 nmol/min/mg enzyme toward linoleoyl-CoA (18:2) for LPGAT activity ; Vmax=3530 nmol/min/mg enzyme toward stearoyl-CoA (18:0) for LPGAT activity ; Vmax=1673 nmol/min/mg enzyme toward palmitoyl-CoA (16:0) for LPGAT activity ;
Catalytic Activity Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Caution PubMed:16620771 does not detect acyl-CoA
Domain The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Function Acyl-CoA
Pathway Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Similarity Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
Subcellular Location Endoplasmic reticulum membrane ; Multi-pass membrane protein .
Tissue Specificity Expressed at higher level in heart, kidney and pancreas than in brain, spleen, liver, lung, small intestine and placenta. {ECO

Identical and Related Proteins

Unique RefSeq proteins for LMP006280 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
42558246 RefSeq NP_872357 414 lysocardiolipin acyltransferase 1 isoform 1
50659059 RefSeq NP_001002257 376 lysocardiolipin acyltransferase 1 isoform 2

Identical Sequences to LMP006280 proteins

Reference Database Accession Length Protein Name
GI:50659059 GenBank AAI46818.1 376 Lysocardiolipin acyltransferase 1 [Homo sapiens]
GI:42558246 GenBank ADL91404.1 414 Sequence 314 from patent US 7709602
GI:42558246 GenBank ADL91714.1 414 Sequence 314 from patent US 7709603
GI:42558246 GenBank ADL97795.1 414 Sequence 314 from patent US 7718770
GI:42558246 GenBank AEU55242.1 414 Sequence 314 from patent US 8063186
GI:50659059 GenBank AHD75217.1 376 Sequence 16496 from patent US 8586006
GI:42558246 GenBank AHD75218.1 414 Sequence 16497 from patent US 8586006
GI:42558246 RefSeq XP_005264301.1 414 PREDICTED: lysocardiolipin acyltransferase 1 isoform X1 [Homo sapiens]
GI:50659059 RefSeq XP_005264302.1 376 PREDICTED: lysocardiolipin acyltransferase 1 isoform X2 [Homo sapiens]
GI:50659059 RefSeq XP_006712044.1 376 PREDICTED: lysocardiolipin acyltransferase 1 isoform X3 [Homo sapiens]

Related Sequences to LMP006280 proteins

Reference Database Accession Length Protein Name
GI:50659059 GenBank ACG89681.1 414 Sequence 314 from patent US 7385033
GI:50659059 GenBank ACG93169.1 414 Sequence 314 from patent US 7388073
GI:50659059 GenBank ACH00908.1 414 Sequence 102 from patent US 7390876
GI:50659059 GenBank ACH02188.1 414 Sequence 314 from patent US 7390886
GI:50659059 GenBank ACH06338.1 414 Sequence 314 from patent US 7393917
GI:50659059 GenBank ACH06646.1 414 Sequence 102 from patent US 7393931
GI:42558246 GenBank JAA23807.1 414 lysocardiolipin acyltransferase 1 [Pan troglodytes]
GI:42558246 GenBank JAA37885.1 414 lysocardiolipin acyltransferase 1 [Pan troglodytes]
GI:42558246 RefSeq XP_001163327.1 414 PREDICTED: lysocardiolipin acyltransferase 1 isoform X1 [Pan troglodytes]
GI:42558246 RefSeq XP_008950764.1 414 PREDICTED: lysocardiolipin acyltransferase 1 isoform X1 [Pan paniscus]
GI:42558246 RefSeq XP_009440527.1 414 PREDICTED: lysocardiolipin acyltransferase 1 isoform X1 [Pan troglodytes]
GI:42558246 RefSeq XP_009440528.1 414 PREDICTED: lysocardiolipin acyltransferase 1 isoform X1 [Pan troglodytes]