Gene/Proteome Database (LMPD)

LMPD ID
LMP006286
Gene ID
9245
Species
Homo sapiens (Human)
Gene Name
glucosaminyl (N-acetyl) transferase 3, mucin type
Gene Symbol
GCNT3
Synonyms
C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM
Alternate Names
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; hC2GnT-M; C2GnT-mucin type; beta1,6GlcNAc-transferase; beta1,6-N-acetylglucosaminyltransferase-M; core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
Chromosome
15
Map Location
15q21.3
EC Number
2.4.1.102
Summary
This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]
Orthologs
View orthologs and multiple alignments for GCNT3

Proteins

beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
Refseq ID NP_004742
Protein GI 4758422
UniProt ID O95395
mRNA ID NM_004751
Length 438
RefSeq Status REVIEWED
MVQWKRLCQLHYLWALGCYMLLATVALKLSFRLKCDSDHLGLESRESQSQYCRNILYNFLKLPAKRSINCSGVTRGDQEAVLQAILNNLEVKKKREPFTDTHYLSLTRDCEHFKAERKFIQFPLSKEEVEFPIAYSMVIHEKIENFERLLRAVYAPQNIYCVHVDEKSPETFKEAVKAIISCFPNVFIASKLVRVVYASWSRVQADLNCMEDLLQSSVPWKYFLNTCGTDFPIKSNAEMVQALKMLNGRNSMESEVPPKHKETRWKYHFEVVRDTLHLTNKKKDPPPYNLTMFTGNAYIVASRDFVQHVLKNPKSQQLIEWVKDTYSPDEHLWATLQRARWMPGSVPNHPKYDISDMTSIARLVKWQGHEGDIDKGAPYAPCSGIHQRAICVYGAGDLNWMLQNHHLLANKFDPKVDDNALQCLEEYLRYKAIYGTEL

Gene Information

Entrez Gene ID
9245
Gene Name
glucosaminyl (N-acetyl) transferase 3, mucin type
Gene Symbol
GCNT3
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0070062 IDA:UniProt C extracellular vesicular exosome
GO:0000139 TAS:Reactome C Golgi membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 TAS:ProtInc C membrane
GO:0047225 IEA:Ensembl F acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity
GO:0003829 IEA:UniProtKB-EC F beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity
GO:0008109 TAS:ProtInc F N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity
GO:0005975 NAS:ProtInc P carbohydrate metabolic process
GO:0044267 TAS:Reactome P cellular protein metabolic process
GO:0002426 IEA:Ensembl P immunoglobulin production in mucosal tissue
GO:0050892 IEA:Ensembl P intestinal absorption
GO:0060993 IEA:Ensembl P kidney morphogenesis
GO:0016266 TAS:Reactome P O-glycan processing
GO:0043687 TAS:Reactome P post-translational protein modification
GO:0006493 TAS:ProtInc P protein O-linked glycosylation
GO:0048729 IEA:Ensembl P tissue morphogenesis

KEGG Pathway Links

KEGG Pathway ID Description
hsa00512 Mucin type O-Glycan biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_200727 O-linked glycosylation
REACT_115606 O-linked glycosylation of mucins

Domain Information

InterPro Annotations

Accession Description
IPR003406 Glycosyl transferase, family 14

UniProt Annotations

Entry Information

Gene Name
glucosaminyl (N-acetyl) transferase 3, mucin type
Protein Entry
GCNT3_HUMAN
UniProt ID
O95395
Species
Human

Comments

Comment Type Description
Catalytic Activity UDP-N-acetyl-D-glucosamine + beta-D- galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D- galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D- galactosaminyl-R.
Catalytic Activity UDP-N-acetyl-D-glucosamine + beta-D- galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D- glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.
Function Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
Induction By all-trans retinoic acid (ATRA), TNF and IL13/interleukin-13. Strongly down-regulated in colorectal cancer. {ECO
Pathway Protein modification; protein glycosylation.
Ptm N-glycosylated.
Similarity Belongs to the glycosyltransferase 14 family.
Subcellular Location Golgi apparatus membrane ; Single-pass type II membrane protein .
Tissue Specificity Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced. {ECO
Web Resource Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/GCNT3ID44105ch15q21.html";
Web Resource Name=Functional Glycomics Gateway - GTase; Note=Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_544";
Web Resource Name=GGDB; Note=GlycoGene database; URL="http://jcggdb.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=GCNT3";

Identical and Related Proteins

Unique RefSeq proteins for LMP006286 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
4758422 RefSeq NP_004742 438 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Identical Sequences to LMP006286 proteins

Reference Database Accession Length Protein Name
GI:4758422 GenBank EAW77572.1 438 glucosaminyl (N-acetyl) transferase 3, mucin type, isoform CRA_a [Homo sapiens]
GI:4758422 GenBank ABM21534.1 438 mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase [Homo sapiens]
GI:4758422 GenBank ACE50480.1 438 Sequence 2 from patent US 7381554
GI:4758422 GenBank AFD45147.1 438 Sequence 140 from patent US 8129114
GI:4758422 GenBank AHD74261.1 438 Sequence 13627 from patent US 8586006
GI:4758422 GenBank AIC50311.1 438 GCNT3, partial [synthetic construct]

Related Sequences to LMP006286 proteins

Reference Database Accession Length Protein Name
GI:4758422 DBBJ BAG35705.1 438 unnamed protein product [Homo sapiens]
GI:4758422 GenBank ADQ32751.1 438 glucosaminyl (N-acetyl) transferase 3, mucin type, partial [synthetic construct]
GI:4758422 RefSeq XP_003827954.1 438 PREDICTED: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 [Pan paniscus]
GI:4758422 RefSeq XP_003827956.1 438 PREDICTED: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 [Pan paniscus]
GI:4758422 RefSeq XP_004056323.1 438 PREDICTED: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 isoform 2 [Gorilla gorilla gorilla]
GI:4758422 RefSeq XP_004056324.1 438 PREDICTED: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 isoform 3 [Gorilla gorilla gorilla]