Gene/Proteome Database (LMPD)
LMPD ID
LMP006342
Gene ID
Species
Homo sapiens (Human)
Gene Name
ectonucleotide pyrophosphatase/phosphodiesterase 6
Gene Symbol
Synonyms
NPP6
Alternate Names
ectonucleotide pyrophosphatase/phosphodiesterase family member 6; NPP-6; E-NPP 6; GPC-Cpde; B830047L21Rik; glycerophosphocholine cholinephosphodiesterase; choline-specific glycerophosphodiester phosphodiesterase
Chromosome
4
Map Location
4q35.1
EC Number
3.1.4.-
Proteins
| ectonucleotide pyrophosphatase/phosphodiesterase family member 6 precursor | |
|---|---|
| Refseq ID | NP_699174 |
| Protein GI | 23503267 |
| UniProt ID | Q6UWR7 |
| mRNA ID | NM_153343 |
| Length | 440 |
| RefSeq Status | VALIDATED |
| MAVKLGTLLLALALGLAQPASARRKLLVFLLDGFRSDYISDEALESLPGFKEIVSRGVKVDYLTPDFPSLSYPNYYTLMTGRHCEVHQMIGNYMWDPTTNKSFDIGVNKDSLMPLWWNGSEPLWVTLTKAKRKVYMYYWPGCEVEILGVRPTYCLEYKNVPTDINFANAVSDALDSFKSGRADLAAIYHERIDVEGHHYGPASPQRKDALKAVDTVLKYMTKWIQERGLQDRLNVIIFSDHGMTDIFWMDKVIELNKYISLNDLQQVKDRGPVVSLWPAPGKHSEIYNKLSTVEHMTVYEKEAIPSRFYYKKGKFVSPLTLVADEGWFITENREMLPFWMNSTGRREGWQRGWHGYDNELMDMRGIFLAFGPDFKSNFRAAPIRSVDVYNVMCNVVGITPLPNNGSWSRVMCMLKGRASTAPPVWPSHCALALILLFLLA | |
| sig_peptide: 1..22 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2123 peptide sequence: MAVKLGTLLLALALGLAQPASA mat_peptide: 23..419 product: Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q6UWR7.2) calculated_mol_wt: 45908 peptide sequence: RRKLLVFLLDGFRSDYISDEALESLPGFKEIVSRGVKVDYLTPDFPSLSYPNYYTLMTGRHCEVHQMIGNYMWDPTTNKSFDIGVNKDSLMPLWWNGSEPLWVTLTKAKRKVYMYYWPGCEVEILGVRPTYCLEYKNVPTDINFANAVSDALDSFKSGRADLAAIYHERIDVEGHHYGPASPQRKDALKAVDTVLKYMTKWIQERGLQDRLNVIIFSDHGMTDIFWMDKVIELNKYISLNDLQQVKDRGPVVSLWPAPGKHSEIYNKLSTVEHMTVYEKEAIPSRFYYKKGKFVSPLTLVADEGWFITENREMLPFWMNSTGRREGWQRGWHGYDNELMDMRGIFLAFGPDFKSNFRAAPIRSVDVYNVMCNVVGITPLPNNGSWSRVMCMLKGRAS | |
Gene Information
Entrez Gene ID
Gene Name
ectonucleotide pyrophosphatase/phosphodiesterase 6
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0031225 | IEA:UniProtKB-KW | C | anchored component of membrane |
| GO:0005576 | IDA:UniProtKB | C | extracellular region |
| GO:0070062 | IDA:UniProt | C | extracellular vesicular exosome |
| GO:0005886 | IDA:UniProtKB | C | plasma membrane |
| GO:0047390 | IDA:UniProtKB | F | glycerophosphocholine cholinephosphodiesterase activity |
| GO:0008889 | IEA:Ensembl | F | glycerophosphodiester phosphodiesterase activity |
| GO:0008081 | IDA:UniProtKB | F | phosphoric diester hydrolase activity |
| GO:0019695 | IDA:UniProtKB | P | choline metabolic process |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0006629 | IDA:UniProtKB | P | lipid metabolic process |
KEGG Pathway Links
Domain Information
UniProt Annotations
Entry Information
Gene Name
ectonucleotide pyrophosphatase/phosphodiesterase 6
Protein Entry
ENPP6_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=344 uM for GPC ; KM=296 uM for 12:0-LPC ; KM=440 uM for 14:0-LPC ; KM=165 uM for 16:0-LPC ; KM=434 uM for 18:2-LPC ; KM=563 uM for 20:4-LPC ; Vmax=484 nmol/min/mg enzyme with GPC as substrate ; Vmax=463 nmol/min/mg enzyme with 12:0-LPC as substrate ; Vmax=367 nmol/min/mg enzyme with 14:0-LPC as substrate ; Vmax=89 nmol/min/mg enzyme with 16:0-LPC as substrate ; Vmax=285 nmol/min/mg enzyme with 18:2-LPC as substrate ; Vmax=470 nmol/min/mg enzyme with 20:4-LPC as substrate ; pH dependence: Optimum pH is 8.5-7.5. ; |
| Catalytic Activity | A lysophosphatidylcholine + H(2)O = a monoacylglycerol + phosphocholine. |
| Catalytic Activity | sn-glycero-3-phosphocholine + H(2)O = glycerol + phosphocholine. |
| Cofactor | Name=a divalent metal cation; Xref=ChEBI |
| Enzyme Regulation | Inhibited by EDTA and EGTA in vitro. |
| Function | Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'- monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid. |
| Similarity | Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. |
| Subcellular Location | Cell membrane ; Lipid-anchor, GPI-anchor . Note=A small amount of the protein may be found in the extracellular milieu. |
| Subunit | Homodimer; disulfide-linked. |
| Tissue Specificity | Predominantly expressed in kidney and brain. In the kidney, expressed specifically in the proximal tubules and thin descending limbs of Henle (at protein level). |
Identical and Related Proteins
Unique RefSeq proteins for LMP006342 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 23503267 | RefSeq | NP_699174 | 440 | ectonucleotide pyrophosphatase/phosphodiesterase family member 6 precursor |
Identical Sequences to LMP006342 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:23503267 | GenBank | ABE15568.1 | 440 | Sequence 3101 from patent US 6979557 |
| GI:23503267 | GenBank | EAX04683.1 | 440 | ectonucleotide pyrophosphatase/phosphodiesterase 6 [Homo sapiens] |
| GI:23503267 | GenBank | AFC06211.1 | 440 | Sequence 3 from patent US 8110660 |
| GI:23503267 | GenBank | AHD74914.1 | 440 | Sequence 15618 from patent US 8586006 |
| GI:23503267 | GenBank | AIC53069.1 | 440 | ENPP6, partial [synthetic construct] |
| GI:23503267 | SwissProt | Q6UWR7.2 | 440 | RecName: Full=Ectonucleotide pyrophosphatase/phosphodiesterase family member 6; Short=E-NPP 6; Short=NPP-6; AltName: Full=Choline-specific glycerophosphodiester phosphodiesterase; AltName: Full=Glycerophosphocholine cholinephosphodiesterase; Short=GPC-Cpde; Flags: Precursor [Homo sapiens] |
Related Sequences to LMP006342 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:23503267 | GenBank | AAQ89039.1 | 440 | AVKL1889 [Homo sapiens] |
| GI:23503267 | GenBank | ABN22224.1 | 440 | Sequence 442 from patent US 7153939 |
| GI:23503267 | GenBank | ACK00041.1 | 440 | Sequence 442 from patent US 7425605 |
| GI:23503267 | GenBank | ACM95151.1 | 440 | Sequence 442 from patent US 7465786 |
| GI:23503267 | GenBank | ACP73906.1 | 440 | Sequence 442 from patent US 7495083 |
| GI:23503267 | GenBank | ACS06532.1 | 440 | Sequence 442 from patent US 7534856 |