Gene/Proteome Database (LMPD)

LMPD ID
LMP006519
Gene ID
72084
Species
Mus musculus (Mouse)
Gene Name
phosphatidylinositol glycan anchor biosynthesis, class X
Gene Symbol
Pigx
Synonyms
2010319C14Rik; PIG-X
Alternate Names
phosphatidylinositol-glycan biosynthesis class X protein; phosphatidylinositol glycan, class X
Chromosome
16
Map Location
16 B2|16
Summary
This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for Pigx

Proteins

phosphatidylinositol-glycan biosynthesis class X protein isoform 1 precursor
Refseq ID NP_077784
Protein GI 161377435
UniProt ID Q99LV7
mRNA ID NM_024464
Length 254
RefSeq Status REVIEWED
MAAGAVAWLLLWAAWLVGRLAADFSDAPFSAGKGVRATCSEIILRQEFLKDGFHRDLLIKVKFGESIEDLQTCRLLIKHYIPPGLFVDPYELASLRERNLTEAVMLSESFNIEAPNYLSNESAVLIYARQDAQCIDCFQAFLPVHYRYHRPHKKDGDTLIVVNNPDLLMYCDQEFPILKCWAQSEVAAPCALKSEEICQWKSMQYKSILKNLTVQVPVGLTIHTSLVCSVTLLITILCSTLILLAVFKYGHFSL
sig_peptide: 1..22 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2311 peptide sequence: MAAGAVAWLLLWAAWLVGRLAA mat_peptide: 23..252 product: phosphatidylinositol-glycan biosynthesis class X protein isoform 2 calculated_mol_wt: 26167 peptide sequence: DFSDAPFSAGVRATCSEIILRQEFLKDGFHRDLLIKVKFGESIEDLQTCRLLIKHYIPPGLFVDPYELASLRERNLTEAVMLSESFNIEAPNYLSNESAVLIYARQDAQCIDCFQAFLPVHYRYHRPHKKDGDTLIVVNNPDLLMYCDQEFPILKCWAQSEVAAPCALKSEEICQWKSMQYKSILKNLTVQVPVGLTIHTSLVCSVTLLITILCSTLILLAVFKYGHFSL sig_peptide: 1..22 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2311 peptide sequence: MAAGAVAWLLLWAAWLVGRLAA mat_peptide: 23..254 product: phosphatidylinositol-glycan biosynthesis class X protein isoform 1 calculated_mol_wt: 26352 peptide sequence: DFSDAPFSAGKGVRATCSEIILRQEFLKDGFHRDLLIKVKFGESIEDLQTCRLLIKHYIPPGLFVDPYELASLRERNLTEAVMLSESFNIEAPNYLSNESAVLIYARQDAQCIDCFQAFLPVHYRYHRPHKKDGDTLIVVNNPDLLMYCDQEFPILKCWAQSEVAAPCALKSEEICQWKSMQYKSILKNLTVQVPVGLTIHTSLVCSVTLLITILCSTLILLAVFKYGHFSL

Gene Information

Entrez Gene ID
72084
Gene Name
phosphatidylinositol glycan anchor biosynthesis, class X
Gene Symbol
Pigx
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005789 IEA:InterPro C endoplasmic reticulum membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0006506 IEA:UniProtKB-UniPathway P GPI anchor biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ko00563 Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
mmu00563 Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
mmu01100 Metabolic pathways

Domain Information

InterPro Annotations

Accession Description
IPR013233 Glycosylphosphatidylinositol-mannosyltransferase I, PIG-X/PBN1

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol glycan anchor biosynthesis, class X
Protein Entry
PIGX_MOUSE
UniProt ID
Q99LV7
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q99LV7-1; Sequence=Displayed; Name=2; IsoId=Q99LV7-2; Sequence=VSP_019843; Note=No experimental confirmation available.;
Function Essential component of glycosylphosphatidylinositol- mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM (By similarity). {ECO:0000250}.
Pathway Glycolipid biosynthesis; glycosylphosphatidylinositol- anchor biosynthesis.
Ptm N-glycosylated. {ECO:0000250}.
Sequence Caution Sequence=BAC25223.1; Type=Miscellaneous discrepancy; Note=Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.; Evidence={ECO:0000305};
Similarity Belongs to the PIGX family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
Subunit Interacts with PIGM. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP006519 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
161377435 RefSeq NP_077784 254 phosphatidylinositol-glycan biosynthesis class X protein isoform 1 precursor

Identical Sequences to LMP006519 proteins

Reference Database Accession Length Protein Name
GI:161377435 SwissProt Q99LV7.2 254 RecName: Full=Phosphatidylinositol-glycan biosynthesis class X protein; Short=PIG-X; Flags: Precursor [Mus musculus]

Related Sequences to LMP006519 proteins

Reference Database Accession Length Protein Name
GI:161377435 DBBJ BAD61008.1 252 subunit of GPI-mannosyltransferase, partial [Rattus norvegicus]
GI:161377435 RefSeq XP_005344963.1 413 PREDICTED: phosphatidylinositol-glycan biosynthesis class X protein [Microtus ochrogaster]
GI:161377435 RefSeq XP_005340354.1 391 PREDICTED: phosphatidylinositol-glycan biosynthesis class X protein [Ictidomys tridecemlineatus]
GI:161377435 RefSeq XP_006974635.1 382 PREDICTED: phosphatidylinositol-glycan biosynthesis class X protein [Peromyscus maniculatus bairdii]
GI:161377435 RefSeq XP_008836787.1 411 PREDICTED: phosphatidylinositol-glycan biosynthesis class X protein [Nannospalax galili]
GI:161377435 SwissProt Q60GF7.2 252 RecName: Full=Phosphatidylinositol-glycan biosynthesis class X protein; Short=PIG-X; Flags: Precursor [Rattus norvegicus]