Gene/Proteome Database (LMPD)

LMPD ID
LMP006573
Gene ID
20788
Species
Mus musculus (Mouse)
Gene Name
sterol regulatory element binding factor 2
Gene Symbol
Srebf2
Synonyms
AI608257; SREBP-2; SREBP2; SREBP2gc; bHLHd2; lop13; nuc
Chromosome
15
Map Location
15 E1|15

Proteins

sterol regulatory element-binding protein 2
Refseq ID NP_150087
Protein GI 73661204
UniProt ID Q3U1N2
mRNA ID NM_033218
Length 1130
RefSeq Status VALIDATED
MDESSELGVLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGGGSNGGSGNNSSGRGNNGGATDPAVQRSFSQVPLSTFSPSAASPQAPALQVKVSPTPPRATPVLQPRPQPQPQPPAQLQQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSPQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTAPIQTAALQVPTLVGSNGTILTTMPVMMGQEKVPIKQVPGGVKQLDPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDSDVDLKIDDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLGLSFNPLTSLLQWGGAHNTDQHPYSGSGRSVLSLESGAGGWFDWMVPTLLLWLVNGVIVLSVFVKLLVHGEPVIRPHSRPSVTFWRHRKQADLDLAKGDFAAAAANLQTCLSVLGRALPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQRWRATPATAAGFEDEAKSSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKILPSTLIEIHLTAAMGLKTRCGGKLGFLASYFLNRAQSLCGPEHSTVPDSLRWLCHPLGQKFFMERSWSIKSAAKESLYCAQRSPADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSVGFVTSPFSSSSVLRSALGPDVICRWWTSAVTMAISWLQGDDAAVRSRFTEVERVPKALEVTESPLVKAVFYTCRAMHASLSGKADGQQNSFCHCERASGHLWSSLNVSGTTSDPSLNHVIQLFTCDLLLSLRTALWQKQASASQLLGETYHASGTELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRPTQNTKHGEVDTWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRRSCSDCQQMIVKLGGGTAIAAS

Gene Information

Entrez Gene ID
20788
Gene Name
sterol regulatory element binding factor 2
Gene Symbol
Srebf2
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:MGI C cytoplasm
GO:0031410 IEA:UniProtKB-KW C cytoplasmic vesicle
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0016020 IDA:BHF-UCL C membrane
GO:0005730 IEA:Ensembl C nucleolus
GO:0005654 TAS:Reactome C nucleoplasm
GO:0005634 IDA:BHF-UCL C nucleus
GO:0032937 IEA:Ensembl C SREBP-SCAP-Insig complex
GO:0003677 IDA:MGI F DNA binding
GO:0070888 IEA:Ensembl F E-box binding
GO:0000978 IEA:Ensembl F RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0003700 IDA:MGI F sequence-specific DNA binding transcription factor activity
GO:0044212 IDA:MGI F transcription regulatory region DNA binding
GO:0008203 IEA:UniProtKB-KW P cholesterol metabolic process
GO:0090370 IEA:Ensembl P negative regulation of cholesterol efflux
GO:0010886 IEA:Ensembl P positive regulation of cholesterol storage
GO:0045944 IDA:MGI P positive regulation of transcription from RNA polymerase II promoter
GO:2000188 IGI:MGI P regulation of cholesterol homeostasis
GO:0072368 IEA:Ensembl P regulation of lipid transport by negative regulation of transcription from RNA polymerase II promoter
GO:0006355 IDA:MGI P regulation of transcription, DNA-templated
GO:0055098 IEA:Ensembl P response to low-density lipoprotein particle
GO:0006351 IEA:UniProtKB-KW P transcription, DNA-templated

Domain Information

InterPro Annotations

Accession Description
IPR003006 Immunoglobulin/major histocompatibility complex, conserved site
IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain

UniProt Annotations

Entry Information

Gene Name
sterol regulatory element binding factor 2
Protein Entry
Q3U1N2_MOUSE
UniProt ID
Q3U1N2
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1 ; IsoId=Q3U1N2-1; Sequence=Displayed; Name=2 ; IsoId=Q3U1N2-2; Sequence=VSP_052658, VSP_052659; Note=No experimental confirmation available. ;
Disruption Phenotype Death around embryonic day 7-8.
Function Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway. Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
Interaction Q9JM73:Srf; NbExp=3; IntAct=EBI-645275, EBI-493266;
Miscellaneous Mice overexpressing processed Srebf2 show preferential activation of cholesterol over fatty acid synthesis in liver and adipose tissue.
Ptm At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7 (By similarity).
Ptm Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.
Similarity Contains 1 bHLH (basic helix-loop-helix) domain.
Subcellular Location Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, COPII-coated vesicle membrane ; Multi-pass membrane protein . Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Subcellular Location Processed sterol regulatory element-binding protein 2: Nucleus {ECO:0000250|UniProtKB:Q12772, ECO:0000255|PROSITE-ProRule:PRU00981}.
Subunit Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein (By similarity). Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminal domain) with RNF139 (By similarity).

Identical and Related Proteins

Unique RefSeq proteins for LMP006573 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
73661204 RefSeq NP_150087 1130 sterol regulatory element-binding protein 2

Identical Sequences to LMP006573 proteins

Reference Database Accession Length Protein Name
GI:73661204 GenBank AAI67259.1 1130 Sterol regulatory element binding factor 2 [synthetic construct]
GI:73661204 SwissProt Q3U1N2.2 1130 RecName: Full=Sterol regulatory element-binding protein 2; Short=SREBP-2; AltName: Full=Sterol regulatory element-binding transcription factor 2; Contains: RecName: Full=Processed sterol regulatory element-binding protein 2 [Mus musculus]

Related Sequences to LMP006573 proteins

Reference Database Accession Length Protein Name
GI:73661204 GenBank AAK54763.1 1125 sterol regulatory element binding protein 2, partial [Mus musculus]
GI:73661204 GenBank EDL04528.1 1148 sterol regulatory element binding factor 2, isoform CRA_b [Mus musculus]
GI:73661204 RefSeq NP_001028866.1 1133 sterol regulatory element-binding protein 2 [Rattus norvegicus]
GI:73661204 RefSeq XP_006520747.1 1124 PREDICTED: sterol regulatory element-binding protein 2 isoform X1 [Mus musculus]
GI:73661204 RefSeq XP_006520748.1 1100 PREDICTED: sterol regulatory element-binding protein 2 isoform X2 [Mus musculus]
GI:73661204 SwissProt Q3T1I5.1 1133 RecName: Full=Sterol regulatory element-binding protein 2; Short=SREBP-2; AltName: Full=Sterol regulatory element-binding transcription factor 2; Contains: RecName: Full=Processed sterol regulatory element-binding protein 2 [Rattus norvegicus]