Gene/Proteome Database (LMPD)

LMPD ID
LMP006577
Gene ID
83490
Species
Mus musculus (Mouse)
Gene Name
phosphoinositide-3-kinase adaptor protein 1
Gene Symbol
Pik3ap1
Synonyms
1810044J04Rik; BCAP
Alternate Names
phosphoinositide 3-kinase adapter protein 1; B cell phosphoinositide 3-kinase adaptor; B-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1
Chromosome
19
Map Location
19|19 D1

Proteins

phosphoinositide 3-kinase adapter protein 1
Refseq ID NP_113553
Protein GI 13878195
UniProt ID Q9EQ32
mRNA ID NM_031376
Length 811
RefSeq Status PROVISIONAL
MAASGWGRGCDILIFYSPDAEEWCQYLQDLFVSCRQVRSQKTQTYRLVPDASFSAQDLWVFRDARCVLVLLSAGLVGCFGQPGLLPMLQRACHPPQRVVRLLCGVQPGDEDFQAFFPDWAHWQEMTCDDEPETYLAAVRKAISEDSGCDSVTDTEPEDERELPFSKQTNLPPEISPGNLMVVQPDRIRCGAETTVYIIVRCKLDEKVSTEAEFSPEDSPSIRVEGTLENEYTVSVKAPDLSSGNVSLKVYSGDLVVCETTVSYYTDMEEIGNLLSSAANPVEFMCQAFKIVPYNTETLDKLLTESLKNNIPASGLHLFGINQLEEDDMMTNQRDEELPTLLHFAAKYGLKNLTALLLTCPGALQAYSVANKHGHYPNTIAEKHGFRDLRQFIDEYVETVDMLKTHIKEELMQGEEADDVYESMAHLSTDLLMKCSLNPGCDDELYESMAAFAPAATEDLYVEMLQASAGNPVSGESFSRPTKDSMIRKFLEGNSVKPASWEREQHHPYGEELYHIVDEDETFSVDLANRPPVPVPRPEASAPGPPPPPDNEPYISKVFAEKSQERLGNFYVSSESIRKEPLVRPWRDRPPSSIYDPFAGMKTPGQRQLITLQEQVKLGIVNVDEAVLHFKEWQLNQKKRSESFRFQQENLKRLRESITRRRKEKPKSGKHTDLEITVPIRHSQHLPEKVEFGVYESGPRKSVLPARTELRRGDWKTDSMSSTASSTSNRSSTRSLLSVSSGMEGDNEDNEIPEITRSRGPGPTQVDGAPVVTGTPVGTLERPPRVPPRAASQRPLTRESFHPPPPVPPRGR

Gene Information

Entrez Gene ID
83490
Gene Name
phosphoinositide-3-kinase adaptor protein 1
Gene Symbol
Pik3ap1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:MGI C cytoplasm
GO:0005829 IDA:UniProtKB C cytosol
GO:0016020 IDA:UniProtKB C membrane
GO:0005886 IDA:MGI C plasma membrane
GO:0042802 IPI:IntAct F identical protein binding
GO:0036312 IDA:UniProtKB F phosphatidylinositol 3-kinase regulatory subunit binding
GO:0034122 IMP:UniProtKB P negative regulation of toll-like receptor signaling pathway
GO:0014068 IMP:UniProtKB P positive regulation of phosphatidylinositol 3-kinase signaling
GO:0034123 IMP:UniProtKB P positive regulation of toll-like receptor signaling pathway
GO:0050727 IMP:UniProtKB P regulation of inflammatory response
GO:0034134 IMP:UniProtKB P toll-like receptor 2 signaling pathway
GO:0034142 IMP:UniProtKB P toll-like receptor 4 signaling pathway
GO:0034154 IMP:UniProtKB P toll-like receptor 7 signaling pathway
GO:0034162 IMP:UniProtKB P toll-like receptor 9 signaling pathway

KEGG Pathway Links

KEGG Pathway ID Description
mmu04662 B cell receptor signaling pathway
mmu04151 PI3K-Akt signaling pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
5892923 Antigen activates B Cell Receptor (BCR) leading to generation of second messengers

Domain Information

InterPro Annotations

Accession Description
IPR017893 DBB_domain

UniProt Annotations

Entry Information

Gene Name
phosphoinositide-3-kinase adaptor protein 1
Protein Entry
BCAP_MOUSE
UniProt ID
Q9EQ32
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; Synonyms=BCAP-L; IsoId=Q9EQ32-1; Sequence=Displayed; Name=2; Synonyms=BCAP-S; IsoId=Q9EQ32-2; Sequence=VSP_034241; Name=3; IsoId=Q9EQ32-3; Sequence=VSP_034242, VSP_034243;
Disruption Phenotype Mice lacking Pik3ap1 display altered B-cell maturation and impaired immune function. Pik3ap1 depletion has an opposite effect in NK cells by promoting their maturation. Mice lacking Pik3ap1 and Cd19 have severe defects in generation of immature and mature B-cells. Moreover, mice lacking Pik3ap1 display increased IL-10, Il-12 and TNF proinflammatory cytokine secretion upon activation of the Toll-like receptors TLR4, TLR7 and TLR9. {ECO:0000269|PubMed:11877477, ECO:0000269|PubMed:18025150, ECO:0000269|PubMed:18337558, ECO:0000269|PubMed:22187458}.
Domain The DBB domain is required for dimerization.
Function Signaling adapter that contributes to B-cell development by linking B-cell receptor (BCR) signaling to the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor (TLR) signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL. {ECO:0000269|PubMed:11781242, ECO:0000269|PubMed:11877477, ECO:0000269|PubMed:12833156, ECO:0000269|PubMed:18025150, ECO:0000269|PubMed:18337558, ECO:0000269|PubMed:22187458, ECO:0000269|PubMed:22187460}.
Interaction Self; NbExp=3; IntAct=EBI-643949, EBI-643949; P22366:Myd88; NbExp=2; IntAct=EBI-643949, EBI-525108; Q99JY1:Tirap; NbExp=2; IntAct=EBI-643949, EBI-6559589;
Ptm Constitutively phosphorylated. Phosphorylated on tyrosine residues in C-terminal region by ABL1 (By similarity). Phosphorylated on tyrosine residues within the YXXM motifs by BTK and SYK. Isoform 1 and isoform 2 are phosphorylated on tyrosine residues, most likely within the YXXM motifs, via CD19 activation. Toll-like receptor activation induces appearance of a phosphorylated form associated with membranes. {ECO:0000250, ECO:0000269|PubMed:11163197, ECO:0000269|PubMed:18025150, ECO:0000269|PubMed:20728433, ECO:0000269|PubMed:22187458, ECO:0000269|PubMed:22187460}.
Similarity Contains 1 DBB domain. {ECO:0000255|PROSITE- ProRule:PRU00707}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:22187458}. Cell membrane {ECO:0000305|PubMed:22187458}; Peripheral membrane protein {ECO:0000305|PubMed:22187458}.
Subunit Homooligomer (Probable). Interacts (phosphorylated on tyrosine residues within YXXM motifs) with PIK3R1 (via SH2 domain); required for BCR- and TLR-mediated activation of phosphoinositide 3-kinase. Interacts (via polyproline C-terminal region) with ABI1 (via SH3 domain); the interaction promotes phosphorylation of PIK3AP1 by ABL1 (By similarity). May interact with MYD88 and TIRAP. {ECO:0000250, ECO:0000269|PubMed:11163197, ECO:0000269|PubMed:18025150, ECO:0000269|PubMed:22187460, ECO:0000305}.
Tissue Specificity Predominantly expressed in spleen (at protein level). Expressed at lower levels in thymus, liver and lung. Expressed in B-cells, macrophages and natural killer (NK) cells. {ECO:0000269|PubMed:11163197, ECO:0000269|PubMed:18337558}.

Identical and Related Proteins

Unique RefSeq proteins for LMP006577 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
13878195 RefSeq NP_113553 811 phosphoinositide 3-kinase adapter protein 1

Identical Sequences to LMP006577 proteins

Reference Database Accession Length Protein Name
GI:13878195 GenBank AAG48584.1 811 B cell phosphoinositide 3-kinase adaptor [Mus musculus]
GI:13878195 SwissProt Q9EQ32.1 811 RecName: Full=Phosphoinositide 3-kinase adapter protein 1; AltName: Full=B-cell adapter for phosphoinositide 3-kinase; AltName: Full=B-cell phosphoinositide 3-kinase adapter protein 1 [Mus musculus]

Related Sequences to LMP006577 proteins

Reference Database Accession Length Protein Name
GI:13878195 DBBJ BAC40962.1 788 unnamed protein product, partial [Mus musculus]
GI:13878195 DBBJ BAE42191.1 812 unnamed protein product [Mus musculus]
GI:13878195 GenBank AAI13142.1 812 Phosphoinositide-3-kinase adaptor protein 1 [Mus musculus]
GI:13878195 GenBank EDL41856.1 812 phosphoinositide-3-kinase adaptor protein 1 [Mus musculus]
GI:13878195 GenBank AAI69055.1 812 Pik3ap1 protein [Rattus norvegicus]
GI:13878195 RefSeq XP_008758636.1 812 PREDICTED: phosphoinositide 3-kinase adapter protein 1 isoform X1 [Rattus norvegicus]