Gene/Proteome Database (LMPD)
LMPD ID
LMP006585
Gene ID
Species
Mus musculus (Mouse)
Gene Name
phospholipase C, delta 3
Gene Symbol
Synonyms
2610205J15Rik; mKIAA1964
Alternate Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3; PLC-delta-3; phospholipase C-delta-3; phosphoinositide phospholipase C-delta-3; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3
Chromosome
11
Map Location
11 E1|11 66.71 cM
EC Number
3.1.4.11
Proteins
| 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 | |
|---|---|
| Refseq ID | NP_690026 |
| Protein GI | 258645148 |
| UniProt ID | Q8K2J0 |
| mRNA ID | NM_152813 |
| Length | 785 |
| RefSeq Status | VALIDATED |
| MLCGGWKRSRRSPEESRVSAQVAAPLAFPPSPASSDSSTKRPGLRALKKMGLTEDEDVQAMLRGSRLLKIRSRTWHKERLYRLQEDGLSVWFQRRIPRAASKHIFFVQHIEAVREGHQSEGLRRFGGAFAPACCLTIAFKGRRKNLDLAAPTAEEAQRWVRGLAKLRARLDAMSQRERLDHWIHSYLHRADSDQDSKMSFKEIKSLLRMVNVDMNDMYAYRLFKECDHSNNERLEGAEIEAFLRRLLKRPELEEIFRRYSGEDRVLSASELLEFLEDQGEDGATLACAQQLIQTYELNETAKQHELMTLDGFMMYLLSPEGAALNVAHTCVFQDMGQPLAHYFISSSHNTYLTDSQIGGTSSTEAYIRAFAQGCRCVELDCWEGPGGEPVIYHGHTLTSKILFRDVIQAVRDHAFTSSPYPVILSLENHCGLEQQAVMARHLRSILGDMLVTQALDSQNPEELPSPEQLKGRILVKGKKLPAARSEDGRILSDREEEEEEEEEAEEALEAAEQRSRAKQISPELSALAVYCCATRLRTLDPSPGPPQSCTVGSLSERKARKFTREAGTSFVRHNTQQLTRVYPLGLRMNSANYNPQEMWNSGCQLVALNFQTPGYEMDLNTGRFLINGQCGYVLKPAYLRQLNTTFDPECPGPPRTTLAIQVLTAQQLPKLNAEKPSSIVDPLVRVEIHGVPEDCAQKETDYVLNNGFNPCWEQTLQFRLRAPELVLVRFVVEDYDTTSPNDFVGQSTLPLSSLKQGYRHIHLLSKDGASLAPATLFVHIRIQNS | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | IEA:Ensembl | C | cytosol |
| GO:0016020 | IEA:UniProtKB-KW | C | membrane |
| GO:0005509 | IEA:InterPro | F | calcium ion binding |
| GO:0004435 | IEA:UniProtKB-EC | F | phosphatidylinositol phospholipase C activity |
| GO:0004871 | IEA:UniProtKB-KW | F | signal transducer activity |
| GO:0001525 | IGI:MGI | P | angiogenesis |
| GO:0035556 | IEA:InterPro | P | intracellular signal transduction |
| GO:0060716 | IGI:MGI | P | labyrinthine layer blood vessel development |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0042127 | IGI:MGI | P | regulation of cell proliferation |
KEGG Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR028406 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 |
| IPR000008 | C2 domain |
| IPR011992 | EF-hand domain pair |
| IPR001192 | Phosphoinositide phospholipase C family |
| IPR000909 | Phospholipase C, phosphatidylinositol-specific , X domain |
| IPR001711 | Phospholipase C, phosphatidylinositol-specific, Y domain |
| IPR015359 | Phospholipase C, phosphoinositol-specific, EF-hand-like |
| IPR017946 | PLC-like phosphodiesterase, TIM beta/alpha-barrel domain |
| IPR001849 | Pleckstrin homology domain |
| IPR011993 | Pleckstrin homology-like domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. |
| Cofactor | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250}; Note=Binds 3 Ca(2+) ions per subunit. Two of the Ca(2+) ions are bound to the C2 domain. {ECO:0000250}; |
| Disruption Phenotype | Mice lacking Plcd1 and Plcd3 die between 11.5 and 13.5 dpc. They exhibit severe disruption of the normal labyrinth architecture in the placenta and decreased placental vascularization, as well as abnormal proliferation and apoptosis of trophoblasts in the labyrinth area. Furthermore, Plcd1 and Plcd3 double knockout embryos supplied with a normal placenta by the tetraploid aggregation method survive beyond 14.5 dpc, indicating that the embryonic lethality is caused by a defect in trophoblasts. {ECO:0000269|PubMed:16314520}. |
| Domain | The C2 domain is a Ca(2+)-dependent membrane-targeting module. {ECO:0000250}. |
| Domain | The PH domain mediates interaction with the surface membrane by binding to PIP2. {ECO:0000250}. |
| Enzyme Regulation | Strongly activated by phosphatidic acid. Inhibited by phosphatidylethanolamine (PtdEtn), phosphatidylcholine (PtdCho), sphingomyelin and phosphatidylserine (PtdSer) (By similarity). {ECO:0000250}. |
| Function | Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow. {ECO:0000269|PubMed:16314520}. |
| Sequence Caution | Sequence=BAC32829.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAD32589.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305}; Sequence=CAM22088.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; |
| Similarity | Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}. |
| Similarity | Contains 1 PH domain. {ECO:0000305}. |
| Similarity | Contains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}. |
| Similarity | Contains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}. |
| Similarity | Contains 3 EF-hand domains. {ECO:0000305}. |
| Subcellular Location | Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Cytoplasm {ECO:0000250}. Cleavage furrow. Note=Localizes at the cleavage furrow during cytokinesis. |
Identical and Related Proteins
Unique RefSeq proteins for LMP006585 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 258645148 | RefSeq | NP_690026 | 785 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 |
Identical Sequences to LMP006585 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:258645148 | SwissProt | Q8K2J0.2 | 785 | RecName: Full=1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3; AltName: Full=Phosphoinositide phospholipase C-delta-3; AltName: Full=Phospholipase C-delta-3; Short=PLC-delta-3 [Mus musculus] |
Related Sequences to LMP006585 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:258645148 | EMBL | CBF74676.1 | 785 | unnamed protein product [Mus musculus] |
| GI:258645148 | EMBL | CBF62939.1 | 785 | unnamed protein product [Mus musculus] |
| GI:258645148 | EMBL | CBU87815.1 | 785 | unnamed protein product [Mus musculus] |
| GI:258645148 | EMBL | CBU96762.1 | 785 | unnamed protein product [Mus musculus] |
| GI:258645148 | GenBank | AAH31392.1 | 785 | Phospholipase C, delta 3 [Mus musculus] |
| GI:258645148 | GenBank | AGX54750.1 | 785 | Sequence 9856 from patent US 8541208 |