Gene/Proteome Database (LMPD)

LMPD ID
LMP006587
Gene ID
66853
Species
Mus musculus (Mouse)
Gene Name
patatin-like phospholipase domain containing 2
Gene Symbol
Pnpla2
Synonyms
0610039C21Rik; 1110001C14Rik; Atgl; TTS-2.2
Alternate Names
patatin-like phospholipase domain-containing protein 2; desnutrin; triglyceride hydrolase; adipose triglyceride lipase; transport-secretion protein
Chromosome
7
Map Location
7 F5|7
EC Number
3.1.1.3

Proteins

patatin-like phospholipase domain-containing protein 2 isoform 1
Refseq ID NP_001157161
Protein GI 254826780
UniProt ID Q8BJ56
mRNA ID NM_001163689
Length 486
RefSeq Status VALIDATED
MFPRETKWNISFAGCGFLGVYHIGVASCLREHAPFLVANATHIYGASAGALTATALVTGACLGEAGANIIEVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVSDGENVIISHFSSKDELIQANVCSTFIPVYCGLIPPTLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNTSIQFNLRNLYRLSKALFPPEPMVLREMCKQGYRDGLRFLRRNGLLNQPNPLLALPPVVPQEEDAEEAAVVEERAGEEDQLQPYRKDRILEHLPARLNEALLEACVEPKDLMTTLSNMLPVRLATAMMVPYTLPLESAVSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGDHLPSRLSEQVELRRAQSLPSVPLSCATYSEALPNWVRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPDALRMRAPASPTAADPATPQDPPGLPPC
patatin-like phospholipase domain-containing protein 2 isoform 2
Refseq ID NP_080078
Protein GI 254826782
UniProt ID Q8BJ56
mRNA ID NM_025802
Length 430
RefSeq Status VALIDATED
MFPRETKWNISFAGCGFLGVYHIGVASCLREHAPFLVANATHIYGASAGALTATALVTGACLGEAGANIIEVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVSDGENVIISHFSSKDELIQANVCSTFIPVYCGLIPPTLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNTSIQFNLRNLYRLSKALFPPEPMVLREMCKQGYRDGLRFLRRNALLEACVEPKDLMTTLSNMLPVRLATAMMVPYTLPLESAVSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGDHLPSRLSEQVELRRAQSLPSVPLSCATYSEALPNWVRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPDALRMRAPASPTAADPATPQDPPGLPPC

Gene Information

Entrez Gene ID
66853
Gene Name
patatin-like phospholipase domain containing 2
Gene Symbol
Pnpla2
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:MGI C cytosol
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0043231 IEA:Ensembl C intracellular membrane-bounded organelle
GO:0005811 IDA:UniProtKB C lipid particle
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0004806 IDA:UniProtKB F triglyceride lipase activity
GO:0034389 IMP:MGI P lipid particle organization
GO:0019915 IMP:UniProtKB P lipid storage
GO:0010891 IDA:UniProtKB P negative regulation of sequestering of triglyceride
GO:0010898 IDA:UniProtKB P positive regulation of triglyceride catabolic process
GO:0019433 IMP:MGI P triglyceride catabolic process

KEGG Pathway Links

KEGG Pathway ID Description
mmu00561 Glycerolipid metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
5893976 Acyl chain remodeling of DAG and TAG

Domain Information

InterPro Annotations

Accession Description
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase
IPR002641 Patatin/Phospholipase A2-related

UniProt Annotations

Entry Information

Gene Name
patatin-like phospholipase domain containing 2
Protein Entry
PLPL2_MOUSE
UniProt ID
Q8BJ56
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q8BJ56-1; Sequence=Displayed; Name=2; IsoId=Q8BJ56-2; Sequence=VSP_026424; Note=No experimental confirmation available.; Name=3; IsoId=Q8BJ56-3; Sequence=VSP_026422, VSP_026423; Note=No experimental confirmation available.;
Catalytic Activity Triacylglycerol + H(2)O = diacylglycerol + a carboxylate. {ECO:0000269|PubMed:16679289}.
Developmental Stage Increased expression when preadipocytes are induced to differentiate to adipocytes. Not detected in proliferating or confluent preadipocytes. {ECO:0000269|PubMed:15337759, ECO:0000269|PubMed:15550674, ECO:0000269|PubMed:16150821, ECO:0000269|PubMed:16705060}.
Disruption Phenotype Mice show increased adipose mass and triacylglycerol deposition in multiple tissues. They accumulate large amounts of lipid in the heart, causing cardiac dysfunction and premature death. {ECO:0000269|PubMed:16675698}.
Enzyme Regulation Stimulated by PKA-dependent PLIN phosphorylation. {ECO:0000269|PubMed:15550674, ECO:0000269|PubMed:17074755, ECO:0000269|PubMed:17114792}.
Function Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. {ECO:0000269|PubMed:15337759, ECO:0000269|PubMed:15550674, ECO:0000269|PubMed:16150821, ECO:0000269|PubMed:16675698, ECO:0000269|PubMed:16679289, ECO:0000269|PubMed:17074755}.
Induction Transiently induced during fasting. cAMP and glucagon may not be involved in the induction during fasting. Induced by dexamethasone. Down-regulated by insulin, isoprotenerol and TNF- alfa. Expression is not affected by glucose and by growth hormone. Expression is reduced in fasted leptin deficient mouse (ob/ob), an obese mouse model. Expression is not affected in fed ob/ob mouse. {ECO:0000269|PubMed:15337759, ECO:0000269|PubMed:16009485, ECO:0000269|PubMed:16150821, ECO:0000269|PubMed:16380488, ECO:0000269|PubMed:16705060}.
Pathway Glycerolipid metabolism; triacylglycerol degradation.
Ptm Phosphorylation at Ser-406 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity. {ECO:0000269|PubMed:15550674, ECO:0000269|PubMed:22733971}.
Sequence Caution Sequence=BAB22643.1; Type=Frameshift; Positions=301, 317, 460; Evidence={ECO:0000305};
Similarity Contains 1 patatin domain. {ECO:0000305}.
Subcellular Location Lipid droplet. Cell membrane; Single-pass type II membrane protein.
Subunit Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity. Interacts with SERPINF1; interacts at one site of interaction (By similarity). Despite a colocalization in lipid droplets, it probably does not interact with PLIN. Interacts with PLIN5; prevents interaction with ABHD5. {ECO:0000250, ECO:0000269|PubMed:16679289, ECO:0000269|PubMed:17189257, ECO:0000269|PubMed:21148142, ECO:0000269|PubMed:21393244}.
Tissue Specificity Expressed at high levels in white and brown adipose tissue, and to a lesser degree in testis and cardiac muscle. Barely detected in liver, spleen, thymus, kidney, skeletal muscle, and brain. Among the white adipose depots, gonadal fat showed the highest level of expression compared with inguinal and renal white adipose tissues. {ECO:0000269|PubMed:15337759, ECO:0000269|PubMed:15550674, ECO:0000269|PubMed:16150821, ECO:0000269|PubMed:16705060}.

Identical and Related Proteins

Unique RefSeq proteins for LMP006587 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
254826780 RefSeq NP_001157161 486 patatin-like phospholipase domain-containing protein 2 isoform 1
254826782 RefSeq NP_080078 430 patatin-like phospholipase domain-containing protein 2 isoform 2

Identical Sequences to LMP006587 proteins

Reference Database Accession Length Protein Name
GI:254826780 DBBJ BAC27476.1 486 unnamed protein product [Mus musculus]
GI:254826780 GenBank AAS48458.1 486 transport-secretion protein [Mus musculus]
GI:254826780 GenBank AAW81963.1 486 adipose triglyceride lipase [Mus musculus]
GI:254826782 GenBank EDL18089.1 430 patatin-like phospholipase domain containing 2, isoform CRA_b [Mus musculus]
GI:254826780 GenBank EDL18092.1 486 patatin-like phospholipase domain containing 2, isoform CRA_e [Mus musculus]
GI:254826780 SwissProt Q8BJ56.1 486 RecName: Full=Patatin-like phospholipase domain-containing protein 2; AltName: Full=Adipose triglyceride lipase; AltName: Full=Desnutrin [Mus musculus]

Related Sequences to LMP006587 proteins

Reference Database Accession Length Protein Name
GI:254826782 DBBJ BAB22387.1 430 unnamed protein product [Mus musculus]
GI:254826780 DBBJ BAE29364.1 486 unnamed protein product [Mus musculus]
GI:254826780 GenBank AAH64747.1 486 Pnpla2 protein [Mus musculus]
GI:254826780 GenBank AAU33824.1 486 desnutrin [Mus musculus]
GI:254826782 GenBank EDM12063.1 430 patatin-like phospholipase domain containing 2 (predicted), isoform CRA_c [Rattus norvegicus]
GI:254826780 GenBank EGW05549.1 486 Patatin-like phospholipase domain-containing protein 2 [Cricetulus griseus]
GI:254826780 RefSeq XP_003509815.1 486 PREDICTED: patatin-like phospholipase domain-containing protein 2 isoform X1 [Cricetulus griseus]
GI:254826782 RefSeq XP_003749062.1 478 PREDICTED: patatin-like phospholipase domain-containing protein 2 [Rattus norvegicus]
GI:254826782 RefSeq XP_005064261.1 430 PREDICTED: patatin-like phospholipase domain-containing protein 2 isoform X2 [Mesocricetus auratus]
GI:254826782 RefSeq XP_005351581.1 430 PREDICTED: patatin-like phospholipase domain-containing protein 2 isoform X2 [Microtus ochrogaster]
GI:254826782 RefSeq XP_005341689.1 430 PREDICTED: patatin-like phospholipase domain-containing protein 2 isoform X3 [Ictidomys tridecemlineatus]
GI:254826780 RefSeq XP_007609780.1 486 PREDICTED: patatin-like phospholipase domain-containing protein 2 isoform X2 [Cricetulus griseus]