Gene/Proteome Database (LMPD)

LMPD ID
LMP006589
Gene ID
97212
Species
Mus musculus (Mouse)
Gene Name
hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Gene Symbol
Hadha
Synonyms
C77020; Mtpa
Alternate Names
trifunctional enzyme subunit alpha, mitochondrial; TP-alpha
Chromosome
5
Map Location
5 B1|5
EC Number
4.2.1.17

Proteins

trifunctional enzyme subunit alpha, mitochondrial precursor
Refseq ID NP_849209
Protein GI 33859811
UniProt ID Q8BMS1
mRNA ID NM_178878
Length 763
RefSeq Status PROVISIONAL
MVASRAIGSLSRFSAFRILRSRGCICRSFTTSSALLTRTHINYGVKGDVAVIRINSPNSKVNTLNKEVQSEFIEVMNEIWANDQIRSAVLISSKPGCFVAGADINMLSSCTTPQEATRISQEGQRMFEKLEKSPKPVVAAISGSCLGGGLELAIACQYRIATKDRKTVLGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRADRAKKMGLVDQLVEPLGPGIKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLVEKLTTYAMTVPFVRQQVYKTVEEKVKKQTKGLYPAPLKIIDAVKAGLEQGSDAGYLAESQKFGELALTKESKALMGLYNGQVLCKKNKFGAPQKNVQQLAILGAGLMGAGIAQVSVDKGLKTLLKDTTVTGLGRGQQQVFKGLNDKVKKKALTSFERDSIFSNLIGQLDYKGFEKADMVIEAVFEDLGVKHKVLKEVESVTPEHCIFASNTSALPINQIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTDKTSKDTTASAVAVGLRQGKVIIVVKDGPGFYTTRCLAPMMSEVMRILQEGVDPKKLDALTTGFGFPVGAATLADEVGVDVAQHVAEDLGKAFGERFGGGSVELLKQMVSKGFLGRKSGKGFYIYQEGSKNKSLNSEMDNILANLRLPAKPEVSSDEDVQYRVITRFVNEAVLCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAYGTQFTPCQLLLDHANNSSKKFYQ
transit_peptide: 1..36 inference: non-experimental evidence, no additional details recorded note: Mitochondrion (Potential); propagated from UniProtKB/Swiss-Prot (Q8BMS1.1) calculated_mol_wt: 3923 peptide sequence: MVASRAIGSLSRFSAFRILRSRGCICRSFTTSSALL mat_peptide: 37..763 product: Trifunctional enzyme subunit alpha, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q8BMS1.1) calculated_mol_wt: 78766 peptide sequence: TRTHINYGVKGDVAVIRINSPNSKVNTLNKEVQSEFIEVMNEIWANDQIRSAVLISSKPGCFVAGADINMLSSCTTPQEATRISQEGQRMFEKLEKSPKPVVAAISGSCLGGGLELAIACQYRIATKDRKTVLGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRADRAKKMGLVDQLVEPLGPGIKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLVEKLTTYAMTVPFVRQQVYKTVEEKVKKQTKGLYPAPLKIIDAVKAGLEQGSDAGYLAESQKFGELALTKESKALMGLYNGQVLCKKNKFGAPQKNVQQLAILGAGLMGAGIAQVSVDKGLKTLLKDTTVTGLGRGQQQVFKGLNDKVKKKALTSFERDSIFSNLIGQLDYKGFEKADMVIEAVFEDLGVKHKVLKEVESVTPEHCIFASNTSALPINQIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTDKTSKDTTASAVAVGLRQGKVIIVVKDGPGFYTTRCLAPMMSEVMRILQEGVDPKKLDALTTGFGFPVGAATLADEVGVDVAQHVAEDLGKAFGERFGGGSVELLKQMVSKGFLGRKSGKGFYIYQEGSKNKSLNSEMDNILANLRLPAKPEVSSDEDVQYRVITRFVNEAVLCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAYGTQFTPCQLLLDHANNSSKKFYQ

Gene Information

Entrez Gene ID
97212
Gene Name
hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Gene Symbol
Hadha
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016507 IEA:Ensembl C mitochondrial fatty acid beta-oxidation multienzyme complex
GO:0005743 IDA:MGI C mitochondrial inner membrane
GO:0042645 IEA:Ensembl C mitochondrial nucleoid
GO:0005739 IDA:MGI C mitochondrion
GO:0051287 IEA:Ensembl F NAD binding
GO:0003988 IEA:Ensembl F acetyl-CoA C-acyltransferase activity
GO:0004300 TAS:MGI F enoyl-CoA hydratase activity
GO:0000062 IEA:Ensembl F fatty-acyl-CoA binding
GO:0016509 IDA:MGI F long-chain-3-hydroxyacyl-CoA dehydrogenase activity
GO:0016508 IEA:Ensembl F long-chain-enoyl-CoA hydratase activity
GO:0006635 IMP:MGI P fatty acid beta-oxidation
GO:0042493 IEA:Ensembl P response to drug
GO:0032868 IMP:MGI P response to insulin

KEGG Pathway Links

KEGG Pathway ID Description
mmu01200 Carbon metabolism

Domain Information

InterPro Annotations

Accession Description
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding
IPR006180 3-hydroxyacyl-CoA dehydrogenase, conserved site
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like
IPR029045 ClpP/crotonase-like domain
IPR001753 Crotonase_core_superfam
IPR013328 Dehydrogenase, multihelical
IPR018376 Enoyl-CoA hydratase/isomerase, conserved site
IPR012803 Fatty acid oxidation complex, alpha subunit, mitochondrial
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Protein Entry
ECHA_MOUSE
UniProt ID
Q8BMS1
Species
Mouse

Comments

Comment Type Description
Catalytic Activity (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O.
Catalytic Activity A long-chain (S)-3-hydroxyacyl-CoA + NAD(+) = a long-chain 3-oxoacyl-CoA + NADH.
Function Bifunctional subunit.
Pathway Lipid metabolism; fatty acid beta-oxidation.
Ptm Acetylation of Lys-569 and Lys-728 is observed in liver mitochondria from fasted mice but not from fed mice. {ECO:0000269|PubMed:16916647, ECO:0000269|PubMed:23576753, ECO:0000269|PubMed:23806337}.
Similarity In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000305}.
Similarity In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family. {ECO:0000305}.
Subcellular Location Mitochondrion matrix {ECO:0000250}.
Subunit Octamer of 4 alpha (HADHA) and 4 beta (HADHB) subunits. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP006589 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
33859811 RefSeq NP_849209 763 trifunctional enzyme subunit alpha, mitochondrial precursor

Identical Sequences to LMP006589 proteins

Reference Database Accession Length Protein Name
GI:33859811 DBBJ BAE41956.1 763 unnamed protein product [Mus musculus]
GI:33859811 EMBL CBG04581.1 763 unnamed protein product [Mus musculus]
GI:33859811 EMBL CBV18103.1 763 unnamed protein product [Mus musculus]
GI:33859811 GenBank EDL37262.1 763 hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit, isoform CRA_a [Mus musculus]
GI:33859811 GenBank AGX95988.1 763 Sequence 72236 from patent US 8541208
GI:33859811 SwissProt Q8BMS1.1 763 RecName: Full=Trifunctional enzyme subunit alpha, mitochondrial; AltName: Full=TP-alpha; Includes: RecName: Full=Long-chain enoyl-CoA hydratase; Includes: RecName: Full=Long chain 3-hydroxyacyl-CoA dehydrogenase; Flags: Precursor [Mus musculus]

Related Sequences to LMP006589 proteins

Reference Database Accession Length Protein Name
GI:33859811 GenBank AAH37009.1 763 Hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit [Mus musculus]
GI:33859811 GenBank AAH91697.1 763 Hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit [Rattus norvegicus]
GI:33859811 GenBank EDM03000.1 763 rCG61339, isoform CRA_b [Rattus norvegicus]
GI:33859811 GenBank AHH58290.1 763 Sequence 35 from patent US 8617877
GI:33859811 RefSeq NP_570839.2 763 trifunctional enzyme subunit alpha, mitochondrial precursor [Rattus norvegicus]
GI:33859811 SwissProt Q64428.2 763 RecName: Full=Trifunctional enzyme subunit alpha, mitochondrial; AltName: Full=TP-alpha; Includes: RecName: Full=Long-chain enoyl-CoA hydratase; Includes: RecName: Full=Long chain 3-hydroxyacyl-CoA dehydrogenase; Flags: Precursor [Rattus norvegicus]