Gene/Proteome Database (LMPD)

LMPD ID
LMP006591
Gene ID
20698
Species
Mus musculus (Mouse)
Gene Name
sphingosine kinase 1
Gene Symbol
Sphk1
Synonyms
1110006G24Rik; Sk1; Spk1
Alternate Names
sphingosine kinase 1; SK 1; SPK 1
Chromosome
11
Map Location
11 E2|11
EC Number
2.7.1.91
Summary
This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Orthologs
View orthologs and multiple alignments for Sphk1

Proteins

sphingosine kinase 1 isoform 1 precursor
Refseq ID NP_035581
Protein GI 22094105
UniProt ID Q8CI15
mRNA ID NM_011451
Length 388
RefSeq Status REVIEWED
MWWCCVLFVVECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPGGSGNALAASVNHYAGYEQVTNEDLLINCTLLLCRRRLSPMNLLSLHTASGLRLYSVLSLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLRIYQGQLAYLPVGTVASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFVLVLVLLHTHLSSELFAAPMGRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRSQRGVFSVDGELMVCEAVQGQVHPNYLWMVCGSRDAPSGRDSRRGPPPEEP
sphingosine kinase 1 isoform 2
Refseq ID NP_079643
Protein GI 27532969
UniProt ID Q8CI15
mRNA ID NM_025367
Length 382
RefSeq Status REVIEWED
MEPVECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPGGSGNALAASVNHYAGYEQVTNEDLLINCTLLLCRRRLSPMNLLSLHTASGLRLYSVLSLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLRIYQGQLAYLPVGTVASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFVLVLVLLHTHLSSELFAAPMGRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRSQRGVFSVDGELMVCEAVQGQVHPNYLWMVCGSRDAPSGRDSRRGPPPEEP
sig_peptide: 1..18 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2152 peptide sequence: MWWCCVLFVVECPRGLLP
sphingosine kinase 1 isoform 2
Refseq ID NP_001165943
Protein GI 289191342
UniProt ID Q8CI15
mRNA ID NM_001172472
Length 382
RefSeq Status REVIEWED
Protein sequence is identical to GI:27532969 (mRNA isoform)
sphingosine kinase 1 isoform 2
Refseq ID NP_001165944
Protein GI 289191344
UniProt ID Q8CI15
mRNA ID NM_001172473
Length 382
RefSeq Status REVIEWED
Protein sequence is identical to GI:27532969 (mRNA isoform)
sphingosine kinase 1 isoform 3
Refseq ID NP_001165946
Protein GI 289191346
UniProt ID Q8CI15
mRNA ID NM_001172475
Length 381
RefSeq Status REVIEWED
MEPECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPGGSGNALAASVNHYAGYEQVTNEDLLINCTLLLCRRRLSPMNLLSLHTASGLRLYSVLSLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLRIYQGQLAYLPVGTVASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFVLVLVLLHTHLSSELFAAPMGRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRSQRGVFSVDGELMVCEAVQGQVHPNYLWMVCGSRDAPSGRDSRRGPPPEEP

Gene Information

Entrez Gene ID
20698
Gene Name
sphingosine kinase 1
Gene Symbol
Sphk1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0030424 IEA:Ensembl C axon
GO:0005737 ISS:UniProtKB C cytoplasm
GO:0005829 IEA:Ensembl C cytosol
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0005886 ISS:UniProtKB C plasma membrane
GO:0008021 IEA:Ensembl C synaptic vesicle
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0005516 ISA:MGI F calmodulin binding
GO:0017050 ISS:UniProtKB F D-erythro-sphingosine kinase activity
GO:0004143 IEA:InterPro F diacylglycerol kinase activity
GO:0003677 ISA:MGI F DNA binding
GO:0000287 ISS:UniProtKB F magnesium ion binding
GO:0003951 IEA:InterPro F NAD+ kinase activity
GO:0008481 ISA:MGI F sphinganine kinase activity
GO:0001568 IGI:MGI P blood vessel development
GO:0007420 IGI:MGI P brain development
GO:0019722 IEA:Ensembl P calcium-mediated signaling
GO:0071363 IEA:Ensembl P cellular response to growth factor stimulus
GO:0070301 IEA:Ensembl P cellular response to hydrogen peroxide
GO:0009267 IEA:Ensembl P cellular response to starvation
GO:0019371 IEA:Ensembl P cyclooxygenase pathway
GO:0007565 IEA:Ensembl P female pregnancy
GO:0006954 IMP:MGI P inflammatory response
GO:0046834 ISA:GOC P lipid phosphorylation
GO:0043066 IEA:Ensembl P negative regulation of apoptotic process
GO:0045766 IEA:Ensembl P positive regulation of angiogenesis
GO:0030307 IEA:Ensembl P positive regulation of cell growth
GO:0030335 IEA:Ensembl P positive regulation of cell migration
GO:0008284 IGI:MGI P positive regulation of cell proliferation
GO:0048146 IEA:Ensembl P positive regulation of fibroblast proliferation
GO:0045931 IEA:Ensembl P positive regulation of mitotic cell cycle
GO:0010976 IEA:Ensembl P positive regulation of neuron projection development
GO:0001956 IEA:Ensembl P positive regulation of neurotransmitter secretion
GO:0042346 ISS:UniProtKB P positive regulation of NF-kappaB import into nucleus
GO:0051092 ISS:UniProtKB P positive regulation of NF-kappaB transcription factor activity
GO:0001934 IEA:Ensembl P positive regulation of protein phosphorylation
GO:0031398 ISS:UniProtKB P positive regulation of protein ubiquitination
GO:0045987 IEA:Ensembl P positive regulation of smooth muscle contraction
GO:0007205 IEA:InterPro P protein kinase C-activating G-protein coupled receptor signaling pathway
GO:0032651 IMP:MGI P regulation of interleukin-1 beta production
GO:0010803 ISS:UniProtKB P regulation of tumor necrosis factor-mediated signaling pathway
GO:0014075 IEA:Ensembl P response to amine
GO:0033198 IEA:Ensembl P response to ATP
GO:0070555 IEA:Ensembl P response to interleukin-1
GO:0032026 IEA:Ensembl P response to magnesium ion
GO:0032570 IEA:Ensembl P response to progesterone
GO:0006670 ISS:UniProtKB P sphingosine metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
mmu04020 Calcium signaling pathway
mmu04666 Fc gamma R-mediated phagocytosis
mmu05152 Tuberculosis
mmu04370 VEGF signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR016064 ATP-NAD kinase-like domain
IPR001206 Diacylglycerol kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
sphingosine kinase 1
Protein Entry
SPHK1_MOUSE
UniProt ID
Q8CI15
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q8CI15-1; Sequence=Displayed; Name=2; IsoId=Q8CI15-2; Sequence=VSP_033448; Name=3; IsoId=Q8CI15-3; Sequence=VSP_033449;
Catalytic Activity ATP + sphinganine = ADP + sphinganine 1- phosphate.
Catalytic Activity ATP + sphingosine = ADP + sphingosine 1- phosphate.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (By similarity). {ECO:0000250}.
Miscellaneous Sphingosine 1-phosphate stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling. {ECO:0000250}.
Similarity Contains 1 DAGKc domain. {ECO:0000255|PROSITE- ProRule:PRU00783}.
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner. {ECO:0000250}.
Subunit Binds to calmodulin. Interacts with SPHKAP. Interacts with CIB1, the interaction occurs in a calcium-dependent manner (By similarity). Interacts with ACY1. {ECO:0000250, ECO:0000269|PubMed:15196915}.

Identical and Related Proteins

Unique RefSeq proteins for LMP006591 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
22094105 RefSeq NP_035581 388 sphingosine kinase 1 isoform 1 precursor
27532969 RefSeq NP_079643 382 sphingosine kinase 1 isoform 2
289191346 RefSeq NP_001165946 381 sphingosine kinase 1 isoform 3

Identical Sequences to LMP006591 proteins

Reference Database Accession Length Protein Name
GI:27532969 DBBJ BAE36079.1 382 unnamed protein product [Mus musculus]
GI:22094105 GenBank AAC61698.1 388 sphingosine kinase [Mus musculus]
GI:289191346 GenBank AAL07499.1 381 sphingosine kinase 1a [Mus musculus]
GI:22094105 GenBank AAW06881.1 388 Sequence 15 from patent US 6800470
GI:22094105 GenBank AAW20555.1 388 Sequence 2 from patent US 6830916
GI:27532969 GenBank AAY02219.1 382 Sequence 4 from patent US 6858427
GI:22094105 GenBank ACJ97777.1 388 Sequence 15 from patent US 7419814
GI:22094105 GenBank ADS49015.1 388 Sequence 15 from patent US 7803595
GI:22094105 GenBank AFS96149.1 388 Sequence 15 from patent US 8263349
GI:27532969 RefSeq NP_001165943.1 382 sphingosine kinase 1 isoform 2 [Mus musculus]
GI:27532969 RefSeq NP_001165944.1 382 sphingosine kinase 1 isoform 2 [Mus musculus]
GI:289191346 RefSeq XP_006532733.1 381 PREDICTED: sphingosine kinase 1 isoform X3 [Mus musculus]
GI:289191346 RefSeq XP_006532734.1 381 PREDICTED: sphingosine kinase 1 isoform X4 [Mus musculus]
GI:289191346 RefSeq XP_006532735.1 381 PREDICTED: sphingosine kinase 1 isoform X5 [Mus musculus]
GI:27532969 RefSeq XP_006532736.1 382 PREDICTED: sphingosine kinase 1 isoform X6 [Mus musculus]
GI:27532969 SwissProt Q8CI15.1 382 RecName: Full=Sphingosine kinase 1; Short=SK 1; Short=SPK 1 [Mus musculus]

Related Sequences to LMP006591 proteins

Reference Database Accession Length Protein Name
GI:27532969 DBBJ BAE33198.1 382 unnamed protein product [Mus musculus]
GI:289191346 GenBank AAC61697.1 504 sphingosine kinase, partial [Mus musculus]
GI:27532969 GenBank AAR46514.1 382 Sequence 6 from patent US 6610534
GI:289191346 GenBank AAW20554.1 381 Sequence 1 from patent US 6830916
GI:289191346 GenBank AAW20557.1 381 Sequence 4 from patent US 6830916
GI:22094105 GenBank AAY02219.1 382 Sequence 4 from patent US 6858427
GI:289191346 GenBank EDL34581.1 504 sphingosine kinase 1, isoform CRA_a, partial [Mus musculus]
GI:27532969 GenBank EDL34583.1 505 sphingosine kinase 1, isoform CRA_c, partial [Mus musculus]
GI:22094105 RefSeq NP_079643.2 382 sphingosine kinase 1 isoform 2 [Mus musculus]
GI:22094105 RefSeq NP_001165943.1 382 sphingosine kinase 1 isoform 2 [Mus musculus]
GI:22094105 RefSeq NP_001165944.1 382 sphingosine kinase 1 isoform 2 [Mus musculus]
GI:27532969 RefSeq XP_006532731.1 512 PREDICTED: sphingosine kinase 1 isoform X1 [Mus musculus]
GI:289191346 RefSeq XP_006532732.1 511 PREDICTED: sphingosine kinase 1 isoform X2 [Mus musculus]
GI:27532969 RefSeq XP_006532733.1 381 PREDICTED: sphingosine kinase 1 isoform X3 [Mus musculus]
GI:27532969 RefSeq XP_006532734.1 381 PREDICTED: sphingosine kinase 1 isoform X4 [Mus musculus]
GI:22094105 RefSeq XP_006532736.1 382 PREDICTED: sphingosine kinase 1 isoform X6 [Mus musculus]
GI:22094105 SwissProt Q8CI15.1 382 RecName: Full=Sphingosine kinase 1; Short=SK 1; Short=SPK 1 [Mus musculus]
GI:289191346 SwissProt Q8CI15.1 382 RecName: Full=Sphingosine kinase 1; Short=SK 1; Short=SPK 1 [Mus musculus]