Gene/Proteome Database (LMPD)
LMPD ID
LMP006657
Gene ID
Species
Homo sapiens (Human)
Gene Name
sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3)
Gene Symbol
Synonyms
NET13; NSMASE-3; NSMASE3
Chromosome
2
Map Location
2q21.1
EC Number
3.1.4.12
Summary
Sphingomyelinases (EC 3.1.4.12), such as SMPD4, catalyze the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide (Krut et al., 2006 [PubMed 16517606]).[supplied by OMIM, Mar 2008]
Orthologs
Proteins
| sphingomyelin phosphodiesterase 4 isoform 1 | |
|---|---|
| Refseq ID | NP_060221 |
| Protein GI | 102467484 |
| UniProt ID | Q9NXE4 |
| mRNA ID | NM_017751 |
| Length | 837 |
| RefSeq Status | VALIDATED |
| MTTFGAVAEWRLPSLRRATLWIPQWFAKKAIFNSPLEAAMAFPHLQQPSFLLASLKADSINKPFAQQCQDLVKVIEDFPAKELHTIFPWLVESIFGSLDGVLVGWNLRCLQGRVNPVEYSIVMEFLDPGGPMMKLVYKLQAEDYKFDFPVSYLPGPVKASIQECILPDSPLYHNKVQFTPTGGLGLNLALNPFEYYIFFFALSLITQKPLPVSLHVRTSDCAYFILVDRYLSWFLPTEGSVPPPLSSSPGGTSPSPPPRTPAIPFASYGLHHTSLLKRHISHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQKMQSPHAKESFTPTEEHVLVVRLLLKHLHAFANSLKPEQASPSAHSHATSPLEEFKRAAVPRFVQQKLYLFLQHCFGHWPLDASFRAVLEMWLSYLQPWRYAPDKQAPGSDSQPRCVSEKWAPFVQENLLMYTKLFVGFLNRALRTDLVSPKHALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELVIPHRQHRLFTAPTFTGSFLSPWPPAVTDASFKVKSHVYSLEGQDCKYTPMFGPEARTLVLRLAQLITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRRFEIEYQGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFLGSFCRYHLTEPGLASRHLLSPVGRRQVAGHTRGPRLSLRFLGSYRTLVSLLLAFFVASLFCVGPLPCTLLLTLGYVLYASAMTLLTERGKLHQP | |
| sphingomyelin phosphodiesterase 4 isoform 2 | |
|---|---|
| Refseq ID | NP_060421 |
| Protein GI | 102467481 |
| UniProt ID | B1PBA3 |
| mRNA ID | NM_017951 |
| Length | 866 |
| RefSeq Status | VALIDATED |
| MTTFGAVAEWRLPSLRRATLWIPQWFAKKAIFNSPLEAAMAFPHLQQPSFLLASLKADSINKPFAQQCQDLVKVIEDFPAKELHTIFPWLVESIFGSLDGVLVGWNLRCLQGRVNPVEYSIVMEFLDPGGPMMKLVYKLQAEDYKFDFPVSYLPGPVKASIQECILPDSPLYHNKVQFTPTGGLGLNLALNPFEYYIFFFALSLITQKPLPVSLHVRTSDCAYFILVDRYLSWFLPTEGSVPPPLSSSPGGTSPSPPPRTPAIPFASYGLHHTSLLKRHISHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQKMQSPHAKLEVLHYRLSVSSALYSPAQPSLQALHAYQESFTPTEEHVLVVRLLLKHLHAFANSLKPEQASPSAHSHATSPLEEFKRAAVPRFVQQKLYLFLQHCFGHWPLDASFRAVLEMWLSYLQPWRYAPDKQAPGSDSQPRCVSEKWAPFVQENLLMYTKLFVGFLNRALRTDLVSPKHALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELVIPHRQHRLFTAPTFTGSFLSPWPPAVTDASFKVKSHVYSLEGQDCKYTPMFGPEARTLVLRLAQLITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRRFEIEYQGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFLGSFCRYHLTEPGLASRHLLSPVGRRQVAGHTRGPRLSLRFLGSYRTLVSLLLAFFVASLFCVGPLPCTLLLTLGYVLYASAMTLLTERGKLHQP | |
| sphingomyelin phosphodiesterase 4 isoform 3 | |
|---|---|
| Refseq ID | NP_001164554 |
| Protein GI | 283806604 |
| UniProt ID | Q9NXE4 |
| mRNA ID | NM_001171083 |
| Length | 764 |
| RefSeq Status | VALIDATED |
| MTTFGAVAEWRLPSLRRATLWIPQWFAKKAIFNSPLEAAMAFPHLQQPSFLLASLKADSINKPFAQQCQDLVKVIEDFPAKGPVKASIQECILPDSPLYHNKVQFTPTGGLGLNLALNPFEYYIFFFALSLITQKPLPVSLHVRTSDCAYFILVDRYLSWFLPTEGSVPPPLSSSPGGTSPSPPPRTPAIPFASYGLHHTSLLKRHISHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQKMQSPHAKESFTPTEEHVLVVRLLLKHLHAFANSLKPEQASPSAHSHATSPLEEFKRAAVPRFVQQKLYLFLQHCFGHWPLDASFRAVLEMWLSYLQPWRYAPDKQAPGSDSQPRCVSEKWAPFVQENLLMYTKLFVGFLNRALRTDLVSPKHALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELVIPHRQHRLFTAPTFTGSFLSPWPPAVTDASFKVKSHVYSLEGQDCKYTPMFGPEARTLVLRLAQLITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRRFEIEYQGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFLGSFCRYHLTEPGLASRHLLSPVGRRQVAGHTRGPRLSLRFLGSYRTLVSLLLAFFVASLFCVGPLPCTLLLTLGYVLYASAMTLLTERGKLHQP | |
Gene Information
Entrez Gene ID
Gene Name
sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3)
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IDA:BHF-UCL | C | endoplasmic reticulum |
| GO:0005789 | TAS:Reactome | C | endoplasmic reticulum membrane |
| GO:0005794 | IDA:HGNC | C | Golgi apparatus |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0005802 | IDA:HGNC | C | trans-Golgi network |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0004767 | TAS:Reactome | F | sphingomyelin phosphodiesterase activity |
| GO:0050290 | IDA:HGNC | F | sphingomyelin phosphodiesterase D activity |
| GO:0071356 | IDA:BHF-UCL | P | cellular response to tumor necrosis factor |
| GO:0046513 | IDA:BHF-UCL | P | ceramide biosynthetic process |
| GO:0046475 | IDA:BHF-UCL | P | glycerophospholipid catabolic process |
| GO:0006687 | TAS:Reactome | P | glycosphingolipid metabolic process |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
| GO:0006665 | TAS:Reactome | P | sphingolipid metabolic process |
| GO:0006685 | IDA:HGNC | P | sphingomyelin catabolic process |
KEGG Pathway Links
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| PWY3DJ-11281 | sphingomyelin metabolism |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_116105 | Glycosphingolipid metabolism |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR024129 | Sphingomyelin phosphodiesterase 4 |
UniProt Annotations
Entry Information
Gene Name
sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3)
Protein Entry
NSMA3_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=10; Name=1; IsoId=Q9NXE4-1; Sequence=Displayed; Name=2; IsoId=Q9NXE4-2; Sequence=VSP_022481; Name=3; IsoId=Q9NXE4-3; Sequence=VSP_022480; Note=No experimental confirmation available.; Name=4; IsoId=Q9NXE4-4; Sequence=VSP_022482; Note=No experimental confirmation available.; Name=5; IsoId=Q9NXE4-5; Sequence=VSP_022479, VSP_022483; Note=No experimental confirmation available.; Name=6; IsoId=Q9NXE4-6; Sequence=VSP_044494, VSP_044495, VSP_022481; Name=7; IsoId=Q9NXE4-7; Sequence=VSP_054382, VSP_054385; Note=No experimental confirmation available.; Name=8; IsoId=Q9NXE4-8; Sequence=VSP_044494, VSP_044495, VSP_054386, VSP_022481; Note=No experimental confirmation available.; Name=9; IsoId=Q9NXE4-9; Sequence=VSP_054383, VSP_054384; Note=No experimental confirmation available.; Name=10; IsoId=Q9NXE4-10; Sequence=VSP_055343, VSP_022481; Note=No experimental confirmation available.; |
| Biophysicochemical Properties | pH dependence: Optimum pH is 7.0. ; |
| Catalytic Activity | Sphingomyelin + H(2)O = N-acylsphingosine + phosphocholine. |
| Cofactor | Name=Mg(2+); Xref=ChEBI |
| Enzyme Regulation | Activated by phosphatidylserine and tumor necrosis factor (TNF). Inhibited by scyphostatin. |
| Function | Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. |
| Sequence Caution | Sequence=BAA91368.1; Type=Erroneous initiation; Evidence= ; Sequence=BAA91567.1; Type=Erroneous initiation; Evidence= ; Sequence=BAA92656.1; Type=Erroneous initiation; Evidence= ; |
| Subcellular Location | Endoplasmic reticulum membrane ; Single-pass membrane protein . Golgi apparatus membrane ; Single-pass membrane protein . |
| Tissue Specificity | Widely expressed, with highest levels in heart and skeletal muscle. |
Identical and Related Proteins
Unique RefSeq proteins for LMP006657 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 102467484 | RefSeq | NP_060221 | 837 | sphingomyelin phosphodiesterase 4 isoform 1 |
| 102467481 | RefSeq | NP_060421 | 866 | sphingomyelin phosphodiesterase 4 isoform 2 |
| 283806604 | RefSeq | NP_001164554 | 764 | sphingomyelin phosphodiesterase 4 isoform 3 |
Identical Sequences to LMP006657 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:102467481 | GenBank | ACA48221.1 | 866 | SKNY protein [Homo sapiens] |
Related Sequences to LMP006657 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:102467481 | DBBJ | BAA92656.1 | 889 | KIAA1418 protein, partial [Homo sapiens] |
| GI:283806604 | DBBJ | BAG61230.1 | 764 | unnamed protein product [Homo sapiens] |
| GI:283806604 | GenBank | AAY14883.1 | 798 | unknown [Homo sapiens] |
| GI:102467481 | GenBank | EAW55597.1 | 866 | hypothetical protein FLJ20297, isoform CRA_a [Homo sapiens] |
| GI:102467484 | GenBank | EAW55601.1 | 837 | hypothetical protein FLJ20297, isoform CRA_e [Homo sapiens] |
| GI:102467484 | GenBank | JAA07592.1 | 837 | sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) [Pan troglodytes] |
| GI:102467484 | GenBank | JAA19427.1 | 837 | sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) [Pan troglodytes] |
| GI:102467481 | GenBank | JAA32075.1 | 866 | sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) [Pan troglodytes] |
| GI:102467484 | GenBank | JAA32076.1 | 837 | sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) [Pan troglodytes] |
| GI:102467481 | GenBank | JAA32077.1 | 866 | sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) [Pan troglodytes] |
| GI:102467481 | GenBank | JAA42552.1 | 866 | sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) [Pan troglodytes] |
| GI:102467481 | GenBank | JAA42553.1 | 866 | sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) [Pan troglodytes] |
| GI:102467484 | GenBank | JAA42554.1 | 837 | sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) [Pan troglodytes] |
| GI:102467484 | RefSeq | NP_060421.2 | 866 | sphingomyelin phosphodiesterase 4 isoform 2 [Homo sapiens] |
| GI:283806604 | RefSeq | XP_005334563.1 | 765 | PREDICTED: sphingomyelin phosphodiesterase 4 isoform X3 [Ictidomys tridecemlineatus] |
| GI:283806604 | RefSeq | XP_005636668.1 | 765 | PREDICTED: sphingomyelin phosphodiesterase 4 isoform X5 [Canis lupus familiaris] |
| GI:283806604 | RefSeq | XP_008067016.1 | 765 | PREDICTED: sphingomyelin phosphodiesterase 4 isoform X3 [Tarsius syrichta] |
| GI:283806604 | RefSeq | XP_008997625.1 | 778 | PREDICTED: sphingomyelin phosphodiesterase 4 [Callithrix jacchus] |