Gene/Proteome Database (LMPD)

LMPD ID
LMP006684
Gene ID
26007
Species
Homo sapiens (Human)
Gene Name
dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Gene Symbol
DAK
Synonyms
NET45
Alternate Names
bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); DHA kinase; glycerone kinase; Dha kinase/FMN cyclase; FAD-AMP lyase cyclizing; FAD-AMP lyase cyclic FMN forming; ATP-dependent dihydroxyacetone kinase
Chromosome
11
Map Location
11q12.2
EC Number
2.7.1.28
Summary
This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for DAK

Proteins

bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)
Refseq ID NP_056348
Protein GI 20149621
UniProt ID Q3LXA3
mRNA ID NM_015533
Length 575
RefSeq Status REVIEWED
MTSKKLVNSVAGCADDALAGLVACNPNLQLLQGHRVALRSDLDSLKGRVALLSGGGSGHEPAHAGFIGKGMLTGVIAGAVFTSPAVGSILAAIRAVAQAGTVGTLLIVKNYTGDRLNFGLAREQARAEGIPVEMVVIGDDSAFTVLKKAGRRGLCGTVLIHKVAGALAEAGVGLEEIAKQVNVVAKAMGTLGVSLSSCSVPGSKPTFELSADEVELGLGIHGEAGVRRIKMATADEIVKLMLDHMTNTTNASHVPVQPGSSVVMMVNNLGGLSFLELGIIADATVRSLEGRGVKIARALVGTFMSALEMPGISLTLLLVDEPLLKLIDAETTAAAWPNVAAVSITGRKRSRVAPAEPQEAPDSTAAGGSASKRMALVLERVCSTLLGLEEHLNALDRAAGDGDCGTTHSRAARAIQEWLKEGPPPASPAQLLSKLSVLLLEKMGGSSGALYGLFLTAAAQPLKAKTSLPAWSAAMDAGLEAMQKYGKAAPGDRTMLDSLWAAGQELQAWKSPGADLLQVLTKAVKSAEAAAEATKNMEAGAGRASYISSARLEQPDPGAVAAAAILRAILEVLQS

Gene Information

Entrez Gene ID
26007
Gene Name
dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Gene Symbol
DAK
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 TAS:Reactome C cytosol
GO:0070062 IDA:UniProtKB C extracellular vesicular exosome
GO:0005634 IDA:UniProt C nucleus
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0034012 IDA:UniProt F FAD-AMP lyase (cyclizing) activity
GO:0004371 IDA:UniProt F glycerone kinase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0050354 IDA:UniProt F triokinase activity
GO:0046835 IDA:UniProt P carbohydrate phosphorylation
GO:0044262 IDA:UniProt P cellular carbohydrate metabolic process
GO:0006071 IEA:InterPro P glycerol metabolic process
GO:0045087 TAS:Reactome P innate immune response
GO:0045088 IDA:UniProt P regulation of innate immune response

KEGG Pathway Links

KEGG Pathway ID Description
hsa01200 Carbon metabolism
hsa00561 Glycerolipid metabolism
hsa04622 RIG-I-like receptor signaling pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_25359 RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways

Domain Information

InterPro Annotations

Accession Description
IPR004006 Dak kinase
IPR004007 DhaL domain
IPR012734 Dihydroxyacetone kinase

UniProt Annotations

Entry Information

Gene Name
dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Protein Entry
DHAK_HUMAN
UniProt ID
Q3LXA3
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q3LXA3-1; Sequence=Displayed; Name=2; IsoId=Q3LXA3-2; Sequence=VSP_057181; Note=Inactive as DHA kinase and FMN cyclase;
Biophysicochemical Properties Kinetic parameters: KM=0.5 uM for dihydroxyacetone ; KM=11 uM for glyceraldehyde ; pH dependence: Optimum pH is 6.6. ;
Catalytic Activity ATP + D-glyceraldehyde = ADP + D- glyceraldehyde 3-phosphate.
Catalytic Activity ATP + glycerone = ADP + glycerone phosphate.
Catalytic Activity FAD = AMP + riboflavin cyclic-4',5'-phosphate.
Cofactor Name=Mg(2+); Xref=ChEBI
Cofactor Name=Mn(2+); Xref=ChEBI
Enzyme Regulation Each activity is inhibited by the substrate(s) of the other.
Function Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.
Similarity Belongs to the dihydroxyacetone kinase (DAK) family.
Similarity Contains 1 DhaK domain. {ECO
Similarity Contains 1 DhaL domain. {ECO
Subunit Homodimer.
Tissue Specificity Detected in erythrocytes (at protein level).

Identical and Related Proteins

Unique RefSeq proteins for LMP006684 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
20149621 RefSeq NP_056348 575 bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)

Identical Sequences to LMP006684 proteins

Reference Database Accession Length Protein Name
GI:20149621 GenBank JAA22704.1 575 dihydroxyacetone kinase 2 homolog [Pan troglodytes]
GI:20149621 GenBank JAA37778.1 575 dihydroxyacetone kinase 2 homolog [Pan troglodytes]
GI:20149621 GenBank AGX54671.1 575 Sequence 9696 from patent US 8541208
GI:20149621 GenBank AHD69436.1 575 Sequence 450 from patent US 8586006
GI:20149621 GenBank AIC62725.1 575 DAK, partial [synthetic construct]
GI:20149621 RefSeq XP_006718559.1 575 PREDICTED: bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing) isoform X3 [Homo sapiens]

Related Sequences to LMP006684 proteins

Reference Database Accession Length Protein Name
GI:20149621 GenBank AAP66248.1 575 Sequence 14 from patent US 6558935
GI:20149621 GenBank AAQ02445.1 576 DKFZP586B1621 protein, partial [synthetic construct]
GI:20149621 GenBank ABA10576.1 575 Dha kinase/FMN cyclase [Homo sapiens]
GI:20149621 GenBank EAW73938.1 575 dihydroxyacetone kinase 2 homolog (yeast), isoform CRA_a [Homo sapiens]
GI:20149621 GenBank EAW73940.1 575 dihydroxyacetone kinase 2 homolog (yeast), isoform CRA_a [Homo sapiens]
GI:20149621 GenBank AED45370.1 575 Sequence 930 from patent US 7892730