Gene/Proteome Database (LMPD)
LMPD ID
LMP006784
Gene ID
Species
Homo sapiens (Human)
Gene Name
diacylglycerol kinase, delta 130kDa
Gene Symbol
Synonyms
DGKdelta; dgkd-2
Alternate Names
diacylglycerol kinase delta; DGK-delta; DAG kinase delta; diglyceride kinase delta
Chromosome
2
Map Location
2q37.1
EC Number
2.7.1.107
Summary
This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| diacylglycerol kinase delta isoform 1 | |
|---|---|
| Refseq ID | NP_003639 |
| Protein GI | 25777596 |
| UniProt ID | Q16760 |
| mRNA ID | NM_003648 |
| Length | 1170 |
| RefSeq Status | REVIEWED |
| MNMFLYFQTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA | |
| diacylglycerol kinase delta isoform 2 | |
|---|---|
| Refseq ID | NP_690618 |
| Protein GI | 25777598 |
| UniProt ID | Q16760 |
| mRNA ID | NM_152879 |
| Length | 1214 |
| RefSeq Status | REVIEWED |
| MAAAAGAPPPGPPQPPPPPPPEESSDSEPEAEPGSPQKLIRKVSTSGQIRQKTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA | |
Gene Information
Entrez Gene ID
Gene Name
diacylglycerol kinase, delta 130kDa
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:UniProtKB | C | cytoplasm |
| GO:0016023 | IDA:MGI | C | cytoplasmic membrane-bounded vesicle |
| GO:0005886 | IDA:MGI | C | plasma membrane |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0003951 | IEA:InterPro | F | NAD+ kinase activity |
| GO:0019992 | NAS:UniProtKB | F | diacylglycerol binding |
| GO:0004143 | TAS:UniProtKB | F | diacylglycerol kinase activity |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0046982 | IDA:MGI | F | protein heterodimerization activity |
| GO:0042803 | IDA:MGI | F | protein homodimerization activity |
| GO:0007596 | TAS:Reactome | P | blood coagulation |
| GO:0016049 | NAS:UniProtKB | P | cell growth |
| GO:0046339 | NAS:UniProtKB | P | diacylglycerol metabolic process |
| GO:0006897 | IEA:UniProtKB-KW | P | endocytosis |
| GO:0007173 | NAS:UniProtKB | P | epidermal growth factor receptor signaling pathway |
| GO:0007275 | NAS:UniProtKB | P | multicellular organismal development |
| GO:0030168 | TAS:Reactome | P | platelet activation |
| GO:0051260 | IDA:UniProtKB | P | protein homooligomerization |
| GO:0007205 | NAS:UniProtKB | P | protein kinase C-activating G-protein coupled receptor signaling pathway |
| GO:0015031 | IEA:UniProtKB-KW | P | protein transport |
| GO:0010033 | IDA:UniProtKB | P | response to organic substance |
| GO:0019932 | NAS:UniProtKB | P | second-messenger-mediated signaling |
| GO:0007165 | TAS:UniProtKB | P | signal transduction |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa00561 | Glycerolipid metabolism |
| hsa00564 | Glycerophospholipid metabolism |
| hsa01100 | Metabolic pathways |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_19184 | GPCR downstream signaling |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR016064 | ATP-NAD kinase-like domain |
| IPR000756 | Diacylglycerol kinase, accessory domain |
| IPR001206 | Diacylglycerol kinase, catalytic domain |
| IPR001849 | Pleckstrin homology domain |
| IPR011993 | Pleckstrin homology-like domain |
| IPR002219 | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain |
| IPR001660 | Sterile alpha motif domain |
| IPR011510 | Sterile alpha motif, type 2 |
| IPR013761 | Sterile alpha motif/pointed domain |
UniProt Annotations
Entry Information
Gene Name
diacylglycerol kinase, delta 130kDa
Protein Entry
DGKD_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=2; IsoId=Q16760-1; Sequence=Displayed; Name=1; IsoId=Q16760-2; Sequence=VSP_012891; |
| Catalytic Activity | ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2- diacyl-sn-glycerol 3-phosphate. |
| Enzyme Regulation | Partially inhibited by phosphatidylserine. |
| Function | Isoform 2 may be involved in cell growth and tumorigenesis. Involved in clathrin-dependent endocytosis. |
| Function | May function as signaling molecule. |
| Interaction | O75923:DYSF; NbExp=3; IntAct=EBI-719333, EBI-2799016; |
| Ptm | Isoform 1 H domain is phosphorylated. |
| Similarity | Belongs to the eukaryotic diacylglycerol kinase family. |
| Similarity | Contains 1 DAGKc domain. {ECO |
| Similarity | Contains 1 PH domain. {ECO |
| Similarity | Contains 1 SAM (sterile alpha motif) domain. |
| Similarity | Contains 2 phorbol-ester/DAG-type zinc fingers. |
| Subcellular Location | Isoform 1: Membrane; Peripheral membrane protein. |
| Subcellular Location | Isoform 2: Cytoplasm. |
| Subunit | The two isoforms are able to form homo- and hetero- oligomer structures (at least tetramers). Isoform 2 interacts with AP2A2. |
| Tissue Specificity | Isoform 2 is ubiquitously expressed also in tumor tissues. Isoform 1 is expressed in ovary, spleen and some tumor-derived cells. |
Identical and Related Proteins
Unique RefSeq proteins for LMP006784 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 25777596 | RefSeq | NP_003639 | 1170 | diacylglycerol kinase delta isoform 1 |
| 25777598 | RefSeq | NP_690618 | 1214 | diacylglycerol kinase delta isoform 2 |
Identical Sequences to LMP006784 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:25777596 | GenBank | EAW71048.1 | 1170 | diacylglycerol kinase, delta 130kDa, isoform CRA_b [Homo sapiens] |
| GI:25777598 | GenBank | EAW71049.1 | 1214 | diacylglycerol kinase, delta 130kDa, isoform CRA_c [Homo sapiens] |
| GI:25777596 | GenBank | EAW71050.1 | 1170 | diacylglycerol kinase, delta 130kDa, isoform CRA_b [Homo sapiens] |
| GI:25777596 | GenBank | AHD72626.1 | 1170 | Sequence 9694 from patent US 8586006 |
| GI:25777598 | GenBank | AHD72627.1 | 1214 | Sequence 9695 from patent US 8586006 |
| GI:25777598 | SwissProt | Q16760.4 | 1214 | RecName: Full=Diacylglycerol kinase delta; Short=DAG kinase delta; AltName: Full=130 kDa diacylglycerol kinase; AltName: Full=Diglyceride kinase delta; Short=DGK-delta [Homo sapiens] |
Related Sequences to LMP006784 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:25777598 | DBBJ | BAC11809.1 | 1214 | diacylglycerol kinase delta2 [Homo sapiens] |
| GI:25777598 | DBBJ | BAA09766.3 | 1198 | KIAA0145 protein, partial [Homo sapiens] |
| GI:25777598 | GenBank | EAW71048.1 | 1170 | diacylglycerol kinase, delta 130kDa, isoform CRA_b [Homo sapiens] |
| GI:25777596 | GenBank | EAW71049.1 | 1214 | diacylglycerol kinase, delta 130kDa, isoform CRA_c [Homo sapiens] |
| GI:25777598 | GenBank | JAB40034.1 | 1214 | diacylglycerol kinase delta isoform 2 [Callithrix jacchus] |
| GI:25777596 | GenBank | AHD72627.1 | 1214 | Sequence 9695 from patent US 8586006 |
| GI:25777598 | RefSeq | NP_003639.2 | 1170 | diacylglycerol kinase delta isoform 1 [Homo sapiens] |
| GI:25777596 | RefSeq | NP_690618.2 | 1214 | diacylglycerol kinase delta isoform 2 [Homo sapiens] |
| GI:25777596 | RefSeq | XP_004033430.1 | 1170 | PREDICTED: diacylglycerol kinase delta [Gorilla gorilla gorilla] |
| GI:25777598 | RefSeq | XP_007964884.1 | 1214 | PREDICTED: diacylglycerol kinase delta isoform X2 [Chlorocebus sabaeus] |
| GI:25777596 | RefSeq | XP_010351446.1 | 1170 | PREDICTED: diacylglycerol kinase delta isoform X1 [Rhinopithecus roxellana] |
| GI:25777596 | SwissProt | Q16760.4 | 1214 | RecName: Full=Diacylglycerol kinase delta; Short=DAG kinase delta; AltName: Full=130 kDa diacylglycerol kinase; AltName: Full=Diglyceride kinase delta; Short=DGK-delta [Homo sapiens] |