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Biophysicochemical Properties
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Kinetic parameters: KM=0.76 mM for CTP {ECO:0000269|PubMed:23623749}; KM=0.067 mM for nitrobenzoxadiazole-phosphatidate {ECO:0000269|PubMed:23623749}; Vmax=68 nmol/min/mg enzyme {ECO:0000269|PubMed:23623749}; pH dependence: Optimum pH is 7-9. {ECO:0000269|PubMed:23623749};
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Catalytic Activity
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CTP + phosphatidate = diphosphate + CDP- diacylglycerol. {ECO:0000269|PubMed:23623749}.
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Caution
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Was initially identified as protein involved in the translocation of transit peptide-containing proteins across the mitochondrial inner membrane by its role in maintaining the integrity and stability of the inner membrane translocase TIM23 complex (PubMed:16943180, PubMed:16790493). It has later been shown that this phenotype can be attributed to the pleiotropic effects of mitochondria lacking cardiolipin (PubMed:19114592). {ECO:0000305|PubMed:16790493, ECO:0000305|PubMed:16943180, ECO:0000305|PubMed:19114592}.
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Cofactor
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Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:23623749}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:23623749}; Name=Cu(2+); Xref=ChEBI:CHEBI:29036; Evidence={ECO:0000269|PubMed:23623749}; Note=Magnesium. Also active with cobalt or copper. {ECO:0000269|PubMed:23623749};
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Disruption Phenotype
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Deficient in cardiolipin. Blocked in the import of presequence-containing proteins as well as of non- cleavable carrier proteins into mitochondria. {ECO:0000269|PubMed:19114592}.
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Function
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Catalyzes the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid (PA) in the mitochondrial inner membrane. Required for the biosynthesis of the dimeric phospholipid cardiolipin, which stabilizes supercomplexes of the mitochondrial respiratory chain in the mitochondrial inner membrane. {ECO:0000269|PubMed:16790493, ECO:0000269|PubMed:16943180, ECO:0000269|PubMed:19114592, ECO:0000269|PubMed:23623749}.
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Interaction
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P19882:HSP60; NbExp=1; IntAct=EBI-23156, EBI-8586;
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Miscellaneous
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Present with 195 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
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Pathway
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Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3. {ECO:0000269|PubMed:19114592, ECO:0000269|PubMed:20485265}.
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Similarity
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Belongs to the TAM41 family. {ECO:0000305}.
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Subcellular Location
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Mitochondrion inner membrane {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16790493, ECO:0000269|PubMed:16943180, ECO:0000269|PubMed:23623749}; Peripheral membrane protein {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16790493, ECO:0000269|PubMed:16943180, ECO:0000269|PubMed:23623749}; Matrix side {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16790493, ECO:0000269|PubMed:16943180, ECO:0000269|PubMed:23623749}.
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