Gene/Proteome Database (LMPD)

LMPD ID
LMP006821
Gene ID
856036
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
farnesyltranstransferase
Gene Symbol
BTS1
Synonyms
-
Alternate Names
farnesyltranstransferase
Chromosome
XVI
EC Number
2.5.1.-

Proteins

farnesyltranstransferase
Refseq ID NP_015256
Protein GI 6325188
UniProt ID Q12051
mRNA ID NM_001183883
Length 335
RefSeq Status PROVISIONAL
MEAKIDELINNDPVWSSQNESLISKPYNHILLKPGKNFRLNLIVQINRVMNLPKDQLAIVSQIVELLHNSSLLIDDIEDNAPLRRGQTTSHLIFGVPSTINTANYMYFRAMQLVSQLTTKEPLYHNLITIFNEELINLHRGQGLDIYWRDFLPEIIPTQEMYLNMVMNKTGGLFRLTLRLMEALSPSSHHGHSLVPFINLLGIIYQIRDDYLNLKDFQMSSEKGFAEDITEGKLSFPIVHALNFTKTKGQTEQHNEILRILLLRTSDKDIKLKLIQILEFDTNSLAYTKNFINQLVNMIKNDNENKYLPDLASHSDTATNLHDELLYIIDHLSEL

Gene Information

Entrez Gene ID
856036
Gene Name
farnesyltranstransferase
Gene Symbol
BTS1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0004161 IEA:UniProtKB-EC F dimethylallyltranstransferase activity
GO:0004311 IDA:SGD F farnesyltranstransferase activity
GO:0004337 IEA:UniProtKB-EC F geranyltranstransferase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0016117 IEA:UniProtKB-KW P carotenoid biosynthetic process
GO:0045337 IEA:UniProtKB-UniPathway P farnesyl diphosphate biosynthetic process
GO:0033384 IEA:UniProtKB-UniPathway P geranyl diphosphate biosynthetic process
GO:0033386 IEA:UniProtKB-UniPathway P geranylgeranyl diphosphate biosynthetic process
GO:0015031 IEA:UniProtKB-KW P protein transport
GO:0016114 IDA:SGD P terpenoid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
sce01110 Biosynthesis of secondary metabolites
sce00900 Terpenoid backbone biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5618346 Cholesterol biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR008949 Isoprenoid synthase domain
IPR000092 Polyprenyl synthetase
IPR017446 Polyprenyl synthetase-related

UniProt Annotations

Entry Information

Gene Name
farnesyltranstransferase
Protein Entry
GGPPS_YEAST
UniProt ID
Q12051
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=3.2 uM for farnesyl diphosphate; KM=0.8 uM for isopentenyl diphosphate;
Catalytic Activity (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Catalytic Activity Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.
Catalytic Activity Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17535895}; Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000269|PubMed:17535895};
Function Catalyzes the trans-addition of the 3 molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate. Required for the membrane attachment of YPT1 and SEC4. May be involved in vesicle trafficking and protein sorting. {ECO:0000269|PubMed:15296494, ECO:0000269|PubMed:7665600}.
Miscellaneous Present with 2840 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.
Pathway Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.
Pathway Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.
Similarity Belongs to the FPP/GGPP synthase family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP006821 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6325188 RefSeq NP_015256 335 farnesyltranstransferase

Identical Sequences to LMP006821 proteins

Reference Database Accession Length Protein Name
GI:6325188 GenBank EHN04357.1 335 Bts1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:6325188 GenBank EIW07043.1 335 Bts1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6325188 GenBank EWG82740.1 335 Bts1p [Saccharomyces cerevisiae R008]
GI:6325188 GenBank EWG92848.1 335 Bts1p [Saccharomyces cerevisiae R103]
GI:6325188 GenBank EWH15430.1 335 Bts1p [Saccharomyces cerevisiae P283]
GI:6325188 gnl McCuskerlabDuke 335 Bts1p [Saccharomyces cerevisiae YJM993]

Related Sequences to LMP006821 proteins

Reference Database Accession Length Protein Name
GI:6325188 PDB 2DH4 340 Chain B, Geranylgeranyl Pyrophosphate Synthase
GI:6325188 PDB 2Z4V 340 Chain A, S. Cerevisiae Geranylgeranyl Pyrophosphate Synthase In Complex With Magnesium And Ggpp (Inhibitory Site)
GI:6325188 PDB 2Z4V 340 Chain B, S. Cerevisiae Geranylgeranyl Pyrophosphate Synthase In Complex With Magnesium And Ggpp (Inhibitory Site)
GI:6325188 PDB 2Z4W 340 Chain A, S. Cerevisiae Geranylgeranyl Pyrophosphate Synthase In Complex With Magnesium And Bph-749
GI:6325188 PDB 2Z4W 340 Chain B, S. Cerevisiae Geranylgeranyl Pyrophosphate Synthase In Complex With Magnesium And Bph-749
GI:6325188 PDB 2Z4X 340 Chain A, S. Cerevisiae Geranylgeranyl Pyrophosphate Synthase In Complex With Magnesium And Bph-252 (P21)