LIPID MAPS

LMPD ID

LMP006828

Gene ID

850445

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Tup1p

Gene Symbol

TUP1

Synonyms

AAR1; AER2; AMM1; CRT4; CYC9; FLK1; ROX4; SFL2; UMR7

Chromosome

III

Tup1p
Refseq ID	NP_010007
Protein GI	6319926
UniProt ID	P16649
mRNA ID	NM_001178790
Length	713
MTASVSNTQNKLNELLDAIRQEFLQVSQEANTYRLQNQKDYDFKMNQQLAEMQQIRNTVYELELTHRKMKDAYEEEIKHLKLGLEQRDHQIASLTVQQQRQQQQQQQVQQHLQQQQQQLAAASASVPVAQQPPATTSATATPAANTTTGSPSAFPVQASRPNLVGSQLPTTTLPVVSSNAQQQLPQQQLQQQQLQQQQPPPQVSVAPLSNTAINGSPTSKETTTLPSVKAPESTLKETEPENNNTSKINDTGSATTATTTTATETEIKPKEEDATPASLHQDHYLVPYNQRANHSKPIPPFLLDLDSQSVPDALKKQTNDYYILYNPALPREIDVELHKSLDHTSVVCCVKFSNDGEYLATGCNKTTQVYRVSDGSLVARLSDDSAANNHRNSITENNTTTSTDNNTMTTTTTTTITTTAMTSAAELAKDVENLNTSSSPSSDLYIRSVCFSPDGKFLATGAEDRLIRIWDIENRKIVMILQGHEQDIYSLDYFPSGDKLVSGSGDRTVRIWDLRTGQCSLTLSIEDGVTTVAVSPGDGKYIAAGSLDRAVRVWDSETGFLVERLDSENESGTGHKDSVYSVVFTRDGQSVVSGSLDRSVKLWNLQNANNKSDSKTPNSGTCEVTYIGHKDFVLSVATTQNDEYILSGSKDRGVLFWDKKSGNPLLMLQGHRNSVISVAVANGSPLGPEYNVFATGSGDCKARIWKYKKIAPN

Entrez Gene ID

850445

Gene Name

Tup1p

Gene Symbol

TUP1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005634	IDA:SGD	C	nucleus
GO:0017053	IDA:SGD	C	transcriptional repressor complex
GO:0001191	IDA:SGD	F	RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription
GO:0042393	IDA:SGD	F	histone binding
GO:0042826	IDA:SGD	F	histone deacetylase binding
GO:0036033	IDA:SGD	F	mediator complex binding
GO:0080025	IDA:SGD	F	phosphatidylinositol-3,5-bisphosphate binding
GO:0043130	IDA:SGD	F	ubiquitin binding
GO:0043486	IMP:SGD	P	histone exchange
GO:0035955	IMP:SGD	P	negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter
GO:0001198	IMP:SGD	P	negative regulation of mating-type specific transcription from RNA polymerase II promoter
GO:0000122	IMP:SGD	P	negative regulation of transcription from RNA polymerase II promoter
GO:0000433	IMP:SGD	P	negative regulation of transcription from RNA polymerase II promoter by glucose
GO:0007070	IMP:SGD	P	negative regulation of transcription from RNA polymerase II promoter during mitosis
GO:0016584	IDA:SGD	P	nucleosome positioning
GO:2000531	IMP:SGD	P	regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter
GO:2001020	IMP:SGD	P	regulation of response to DNA damage stimulus

KEGG Pathway Links

KEGG Pathway ID	Description
ko04111	Cell cycle - yeast
sce04111	Cell cycle - yeast

InterPro Annotations

Accession	Description
IPR020472	G-protein beta WD-40 repeat
IPR013890	Tscrpt_rep_Tup1_N
IPR019775	WD40 repeat, conserved site
IPR017986	WD40-repeat-containing domain
IPR015943	WD40/YVTN repeat-like-containing domain
IPR001680	WD40_repeat

Entry Information

Gene Name

Tup1p

Protein Entry

TUP1_YEAST

UniProt ID

P16649

Species

Yeast (S288c)

Comments

Comment Type	Description
Function	Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex. {ECO:0000269\|PubMed:10722672, ECO:0000269\|PubMed:11069890, ECO:0000269\|PubMed:11172717, ECO:0000269\|PubMed:11230135, ECO:0000269\|PubMed:11784848, ECO:0000269\|PubMed:14665463, ECO:0000269\|PubMed:2247069}.
Interaction	P14922:CYC8; NbExp=5; IntAct=EBI-19654, EBI-18215; P40356:PGD1; NbExp=3; IntAct=EBI-19654, EBI-13268; P48743:RFX1; NbExp=2; IntAct=EBI-19654, EBI-15036; P32561:RPD3; NbExp=2; IntAct=EBI-19654, EBI-15864;
Miscellaneous	Present with 5840 molecules/cell in log phase SD medium
Miscellaneous	Present with 5840 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Sequence Caution	Sequence=CAA34411.1; Type=Erroneous initiation; Evidence= ;
Sequence Caution	Sequence=CAA34411.1; Type=Erroneous initiation; Evidence={ECO:0000305};
Similarity	Belongs to the WD repeat TUP1 family
Similarity	Belongs to the WD repeat TUP1 family. {ECO:0000305}.
Similarity	Contains 7 WD repeats. {ECO:0000255\|PROSITE- ProRule:PRU00221}.
Subcellular Location	Nucleus .
Subcellular Location	Nucleus {ECO:0000269\|PubMed:14562095}.
Subunit	Associates with CYC8/SSN6 to form the CYC8-TUP1 (or TUP1- SSN6) corepressor complex that is composed of 4 copies of TUP1 and one copy of CYC8. Interacts with histone H3, histone H4, MATALPHA2, RFX1, SKO1, PGD1, HDA1, HDA2, HOS1, HOS2 AND RPD3. {ECO:0000269\|PubMed:10722672, ECO:0000269\|PubMed:11069890, ECO:0000269\|PubMed:11172717, ECO:0000269\|PubMed:11500510, ECO:0000269\|PubMed:14525981, ECO:0000269\|PubMed:7995523, ECO:0000269\|PubMed:8675011, ECO:0000269\|PubMed:8943325, ECO:0000269\|PubMed:9741624}.