LIPID MAPS

LMPD ID

LMP006842

Gene ID

852241

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

CTP synthase URA7

Gene Symbol

URA7

Synonyms

-

Chromosome

II

EC Number

6.3.4.2

CTP synthase URA7
Refseq ID	NP_009514
Protein GI	6319432
UniProt ID	P28274
mRNA ID	NM_001178279
Length	579
MKYVVVSGGVISGIGKGVLASSTGMLMKTLGLKVTSIKIDPYMNIDAGTMSPLEHGECFVLDDGGETDLDLGNYERYLGVTLTKDHNITTGKIYSHVIAKERKGDYLGKTVQIVPHLTNAIQDWIERVAKIPVDDTGMEPDVCIIELGGTVGDIESAPFVEALRQFQFKVGKENFALIHVSLVPVIHGEQKTKPTQAAIKGLRSLGLVPDMIACRCSETLDKPTIDKIAMFCHVGPEQVVNVHDVNSTYHVPLLLLEQKMIDYLHARLKLDEISLTEEEKQRGLELLSKWKATTGNFDESMETVKIALVGKYTNLKDSYLSVIKALEHSSMKCRRKLDIKWVEATDLEPEAQESNKTKFHEAWNMVSTADGILIPGGFGVRGTEGMVLAARWARENHIPFLGVCLGLQIATIEFTRSVLGRKDSHSAEFYPDIDEKNHVVVFMPEIDKETMGGSMRLGLRPTFFQNETEWSQIKKLYGDVSEVHERHRHRYEINPKMVDELENNGLIFVGKDDTGKRCEILELKNHPYYIATQYHPEYTSKVLDPSKPFLGLVAASAGILQDVIEGKYDLEAGENKFNF

Entrez Gene ID

852241

Gene Name

CTP synthase URA7

Gene Symbol

URA7

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0097268	IDA:SGD	C	cytoophidium
GO:0005524	IEA:UniProtKB-KW	F	ATP binding
GO:0003883	IDA:SGD	F	CTP synthase activity
GO:0044210	IEA:UniProtKB-UniPathway	P	'de novo' CTP biosynthetic process
GO:0006241	IDA:SGD	P	CTP biosynthetic process
GO:0006541	IEA:UniProtKB-KW	P	glutamine metabolic process
GO:0008654	IMP:SGD	P	phospholipid biosynthetic process
GO:0019856	IMP:SGD	P	pyrimidine nucleobase biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
sce01100	Metabolic pathways
ko00240	Pyrimidine metabolism
sce00240	Pyrimidine metabolism
M00052	Pyrimidine ribonucleotide biosynthesis, UMP => UDP/UTP,CDP/CTP
sce_M00052	Pyrimidine ribonucleotide biosynthesis, UMP => UDP/UTP,CDP/CTP

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-7176	UTP and CTP de novo biosynthesis
PYRIMID-RNTSYN-PWY	de novo biosynthesis of pyrimidine ribonucleotides
PWY0-162	superpathway of pyrimidine ribonucleotides de novo biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618023	Metabolism
5618059	Metabolism of nucleotides
5618062	Synthesis and interconversion of nucleotide di- and triphosphates

InterPro Annotations

Accession	Description
IPR004468	CTP_synthase
IPR017456	CTP_synthase_N
IPR029062	Class I glutamine amidotransferase-like
IPR017926	Glutamine amidotransferase
IPR027417	P-loop containing nucleoside triphosphate hydrolase

Entry Information

Gene Name

CTP synthase URA7

Protein Entry

URA7_YEAST

UniProt ID

P28274

Species

Yeast (S288c)

Comments

Comment Type	Description
Biophysicochemical Properties	Kinetic parameters: KM=60 uM for UTP {ECO:0000269\|PubMed:8075080}; KM=0.9 mM for ATP {ECO:0000269\|PubMed:8075080}; KM=0.4 mM for L-glutamine {ECO:0000269\|PubMed:8075080}; pH dependence: Optimum pH is 8.0. {ECO:0000269\|PubMed:8075080};
Biophysicochemical Properties	Kinetic parameters: KM=60 uM for UTP ; KM=0.9 mM for ATP ; KM=0.4 mM for L-glutamine ; pH dependence: Optimum pH is 8.0. ;
Catalytic Activity	ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.
Enzyme Regulation	Activated by GTP and inhibited by CTP
Enzyme Regulation	Activated by GTP and inhibited by CTP. {ECO:0000269\|PubMed:8075080}.
Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.
Interaction	Q03690:TIF31; NbExp=3; IntAct=EBI-20128, EBI-8989;
Miscellaneous	Present with 57600 molecules/cell in log phase SD medium
Miscellaneous	Present with 57600 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.
Similarity	Belongs to the CTP synthase family
Similarity	Belongs to the CTP synthase family. {ECO:0000305}.
Similarity	Contains 1 glutamine amidotransferase type-1 domain
Similarity	Contains 1 glutamine amidotransferase type-1 domain. {ECO:0000255\|PROSITE-ProRule:PRU00605}.
Subunit	Homodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP
Subunit	Homodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP. {ECO:0000269\|PubMed:8075080}.