Gene/Proteome Database (LMPD)

LMPD ID
LMP006863
Gene ID
853567
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
CTP synthase URA8
Gene Symbol
URA8
Synonyms
-
Chromosome
X
EC Number
6.3.4.2

Proteins

CTP synthase URA8
Refseq ID NP_012637
Protein GI 398365363
UniProt ID P38627
mRNA ID NM_001181761
Length 578
MKYVVVSGGVISGIGKGVLASSTGMLLKTLGLKVTSIKIDPYMNIDAGTMSPLEHGECFVLDDGGETDLDLGNYERYLGITLSRDHNITTGKIYSHVISRERRGDYLGKTVQIVPHLTNAIQDWIQRVSKIPVDDTGLEPDVCIIELGGTVGDIESAPFVEALRQFQFEVGRENFALIHVSLVPVIHGEQKTKPTQAAIKDLRSLGLIPDMIACRCSEELNRSTIDKIAMFCHVGPEQVVNVHDVNSTYHVPLLLLKQHMIDYLHSRLKLGEVPLTLEDKERGSQLLTNWENMTKNLDDSDDVVKIALVGKYTNLKDSYLSVTKSLEHASMKCRRQLEILWVEASNLEPETQEVDKNKFHDSWNKLSSADGILVPGGFGTRGIEGMILAAKWARESGVPFLGVCLGLQVAAIEFARNVIGRPNSSSTEFLDETLLAPEDQVVIYMPEIDKEHMGGTMRLGLRPTIFQPNSEWSNIRKLYGEVNEVHERHRHRYEINPKIVNDMESRGFIFVGKDETGQRCEIFELKGHPYYVGTQYHPEYTSKVLEPSRPFWGLVAAASGTLGEVIKDINLSEGNENE

Gene Information

Entrez Gene ID
853567
Gene Name
CTP synthase URA8
Gene Symbol
URA8
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0097268 IDA:SGD C cytoophidium
GO:0005829 TAS:SGD C cytosol
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0003883 IDA:SGD F CTP synthase activity
GO:0044210 IEA:UniProtKB-UniPathway P 'de novo' CTP biosynthetic process
GO:0006241 IDA:SGD P CTP biosynthetic process
GO:0006541 IEA:UniProtKB-KW P glutamine metabolic process
GO:0008654 TAS:SGD P phospholipid biosynthetic process
GO:0019856 IMP:SGD P pyrimidine nucleobase biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
sce01100 Metabolic pathways
ko00240 Pyrimidine metabolism
sce00240 Pyrimidine metabolism
M00052 Pyrimidine ribonucleotide biosynthesis, UMP => UDP/UTP,CDP/CTP
sce_M00052 Pyrimidine ribonucleotide biosynthesis, UMP => UDP/UTP,CDP/CTP

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-7176 UTP and CTP de novo biosynthesis
PYRIMID-RNTSYN-PWY de novo biosynthesis of pyrimidine ribonucleotides
PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5618023 Metabolism
5618059 Metabolism of nucleotides
5618062 Synthesis and interconversion of nucleotide di- and triphosphates

Domain Information

InterPro Annotations

Accession Description
IPR004468 CTP_synthase
IPR017456 CTP_synthase_N
IPR029062 Class I glutamine amidotransferase-like
IPR017926 Glutamine amidotransferase
IPR027417 P-loop containing nucleoside triphosphate hydrolase

UniProt Annotations

Entry Information

Gene Name
CTP synthase URA8
Protein Entry
URA8_YEAST
UniProt ID
P38627
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=74 uM for UTP {ECO:0000269|PubMed:7559626}; KM=22 uM for ATP {ECO:0000269|PubMed:7559626}; KM=0.14 mM for L-glutamine {ECO:0000269|PubMed:7559626}; pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:7559626};
Biophysicochemical Properties Kinetic parameters: KM=74 uM for UTP ; KM=22 uM for ATP ; KM=0.14 mM for L-glutamine ; pH dependence: Optimum pH is 7.5. ;
Catalytic Activity ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Enzyme Regulation Activated by GTP. Subject to allosteric product inhibition by CTP. Inhibited by p-chloromercuriphenylsulfonic acid, N-ethylmaleimide and cyclopentenylcytosine (CPEC)
Enzyme Regulation Activated by GTP. Subject to allosteric product inhibition by CTP. Inhibited by p-chloromercuriphenylsulfonic acid, N-ethylmaleimide and cyclopentenylcytosine (CPEC). {ECO:0000269|PubMed:7559626, ECO:0000269|PubMed:9668079}.
Function Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Plays an important role in the regulation of phospholipid synthesis.
Miscellaneous Present with 5370 molecules/cell in log phase SD medium
Miscellaneous Present with 5370 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.
Sequence Caution Sequence=CAA89633.1; Type=Frameshift; Positions=559; Evidence= ;
Sequence Caution Sequence=CAA89633.1; Type=Frameshift; Positions=559; Evidence={ECO:0000305};
Similarity Belongs to the CTP synthase family
Similarity Belongs to the CTP synthase family. {ECO:0000305}.
Similarity Contains 1 glutamine amidotransferase type-1 domain
Similarity Contains 1 glutamine amidotransferase type-1 domain. {ECO:0000255|PROSITE-ProRule:PRU00605}.
Subcellular Location Cytoplasm .
Subcellular Location Cytoplasm {ECO:0000269|PubMed:14562095}.
Subunit Homodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP
Subunit Homodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP. {ECO:0000269|PubMed:7559626}.

Identical and Related Proteins

Unique RefSeq proteins for LMP006863 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
398365363 RefSeq NP_012637 578 CTP synthase URA8

Identical Sequences to LMP006863 proteins

Reference Database Accession Length Protein Name

Related Sequences to LMP006863 proteins

Reference Database Accession Length Protein Name