LIPID MAPS

LMPD ID

LMP006878

Gene ID

851513

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

type 2A-related serine/threonine-protein phosphatase SIT4

Gene Symbol

SIT4

Synonyms

PPH1

Chromosome

IV

EC Number

3.1.3.16;

type 2A-related serine/threonine-protein phosphatase SIT4
Refseq ID	NP_010236
Protein GI	6320156
UniProt ID	P20604
mRNA ID	NM_001180106
Length	311
MVSRGPDEWLETIKKCQALTENEMKQLCEMVKELLMEESNIQPVQTPVTVCGDIHGQFHDLLELFRTAGGFPDDINYIFLGDYVDRGYYSLETFTLLMCLKVKYPAKITLVRGNHESRQITQVYGFYEECLNKYGSTTVWKYCCQVFDFLTLAAIIDGKILCVHGGLSPEIRMLDQIRVLSRAQEVPHEGGFSDLLWSDPDNVEAWQVSPRGAGWLFGSKVAREFNHVNGLNLIARAHQLVMEGFKYHFPEKDVVTVWSAPNYCYRCGNVASVMKVDEDLEPTFKIFSAVPDDYIRESTANHNNQRAGYFL

Entrez Gene ID

851513

Gene Name

type 2A-related serine/threonine-protein phosphatase SIT4

Gene Symbol

SIT4

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005737	IDA:SGD	C	cytoplasm
GO:0046872	IEA:UniProtKB-KW	F	metal ion binding
GO:0004722	IMP:SGD	F	protein serine/threonine phosphatase activity
GO:0006281	IMP:SGD	P	DNA repair
GO:0000082	IGI:SGD	P	G1/S transition of mitotic cell cycle
GO:0031929	IMP:SGD	P	TOR signaling
GO:0030036	IMP:SGD	P	actin cytoskeleton organization
GO:0034599	IMP:SGD	P	cellular response to oxidative stress
GO:0016311	IMP:SGD	P	dephosphorylation
GO:0031505	IMP:SGD	P	fungal-type cell wall organization
GO:0035556	IMP:SGD	P	intracellular signal transduction
GO:0007067	IEA:UniProtKB-KW	P	mitotic nuclear division
GO:0001302	IMP:SGD	P	replicative cell aging
GO:0002098	IMP:SGD	P	tRNA wobble uridine modification

InterPro Annotations

Accession	Description
IPR004843	Calcineurin-like phosphoesterase domain, apaH type
IPR029052	Metallo-dependent phosphatase-like
IPR006186	Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase

Entry Information

Gene Name

type 2A-related serine/threonine-protein phosphatase SIT4

Protein Entry

PP11_YEAST

UniProt ID

P20604

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate.
Cofactor	Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ; Note=Binds 2 manganese ions per subunit. ;
Cofactor	Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 manganese ions per subunit. {ECO:0000250};
Developmental Stage	Functions in the late cell cycle G1 phase for progression into the S phase, possibly associated in two separate complexes with the phosphorylated forms of p155 and p190, two high MW proteins.
Function	Involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1. Associates with the SAP proteins in a cell cycle-dependent manner.
Interaction	P40454:RRD1; NbExp=4; IntAct=EBI-13707, EBI-25278; P43612:SAP155; NbExp=8; IntAct=EBI-13707, EBI-16370; P40856:SAP185; NbExp=8; IntAct=EBI-13707, EBI-16384; P36123:SAP190; NbExp=4; IntAct=EBI-13707, EBI-16392; Q04372:TAP42; NbExp=6; IntAct=EBI-13707, EBI-18926;
Miscellaneous	Present with 3970 molecules/cell in log phase SD medium
Miscellaneous	Present with 3970 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily
Similarity	Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily. {ECO:0000305}.
Subcellular Location	Cytoplasm.
Subunit	Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Forms a ternary complex with RRD1-TAP42. {ECO:0000269\|PubMed:12820961, ECO:0000269\|PubMed:14551259, ECO:0000269\|PubMed:15447631, ECO:0000269\|PubMed:15689491}.