Gene/Proteome Database (LMPD)

LMPD ID
LMP006918
Gene ID
855201
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
phosphatidate phosphatase PAH1
Gene Symbol
PAH1
Synonyms
SMP2
Alternate Names
phosphatidate phosphatase PAH1
Chromosome
XIII
EC Number
3.1.3.4

Proteins

phosphatidate phosphatase PAH1
Refseq ID NP_013888
Protein GI 6323817
UniProt ID P32567
mRNA ID NM_001182669
Length 862
RefSeq Status PROVISIONAL
MQYVGRALGSVSKTWSSINPATLSGAIDVIVVEHPDGRLSCSPFHVRFGKFQILKPSQKKVQVFINEKLSNMPMKLSDSGEAYFVFEMGDQVTDVPDELLVSPVMSATSSPPQSPETSILEGGTEGEGEGENENKKKEKKVLEEPDFLDINDTGDSGSKNSETTGSLSPTESSTTTPPDSVEERKLVEQRTKNFQQKLNKKLTEIHIPSKLDNNGDLLLDTEGYKPNKNMMHDTDIQLKQLLKDEFGNDSDISSFIKEDKNGNIKIVNPYEHLTDLSPPGTPPTMATSGSVLGLDAMESGSTLNSLSSSPSGSDTEDETSFSKEQSSKSEKTSKKGTAGSGETEKRYIRTIRLTNDQLKCLNLTYGENDLKFSVDHGKAIVTSKLFVWRWDVPIVISDIDGTITKSDALGHVLAMIGKDWTHLGVAKLFSEISRNGYNILYLTARSAGQADSTRSYLRSIEQNGSKLPNGPVILSPDRTMAALRREVILKKPEVFKIACLNDIRSLYFEDSDNEVDTEEKSTPFFAGFGNRITDALSYRTVGIPSSRIFTINTEGEVHMELLELAGYRSSYIHINELVDHFFPPVSLDSVDLRTNTSMVPGSPPNRTLDNFDSEITSGRKTLFRGNQEEKFTDVNFWRDPLVDIDNLSDISNDDSDNIDEDTDVSQQSNISRNRANSVKTAKVTKAPQRNVSGSTNNNEVLAASSDVENASDLVSSHSSSGSTPNKSTMSKGDIGKQIYLELGSPLASPKLRYLDDMDDEDSNYNRTKSRRASSAAATSIDKEFKKLSVSKAGAPTRIVSKINVSNDVHSLGNSDTESRREQSVNETGRNQLPHNSMDDKDLDSRVSDEFDDDEFDEDEFED

Gene Information

Entrez Gene ID
855201
Gene Name
phosphatidate phosphatase PAH1
Gene Symbol
PAH1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:SGD C cytosol
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0019898 IDA:SGD C extrinsic component of membrane
GO:0031965 IDA:SGD C nuclear membrane
GO:0005773 IDA:SGD C vacuole
GO:0008195 IDA:SGD F phosphatidate phosphatase activity
GO:0044212 IDA:SGD F transcription regulatory region DNA binding
GO:0009060 IMP:SGD P aerobic respiration
GO:0016311 IDA:GOC P dephosphorylation
GO:0034389 IMP:SGD P lipid particle organization
GO:0008654 IMP:SGD P phospholipid biosynthetic process
GO:0006276 IMP:SGD P plasmid maintenance
GO:0006355 IEA:UniProtKB-KW P regulation of transcription, DNA-templated
GO:0006351 IEA:UniProtKB-KW P transcription, DNA-templated
GO:0019432 IMP:SGD P triglyceride biosynthetic process
GO:0042144 IMP:SGD P vacuole fusion, non-autophagic

KEGG Pathway Links

KEGG Pathway ID Description
sce00561 Glycerolipid metabolism
sce00564 Glycerophospholipid metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID Description
TRIGLSYN-PWY triacylglycerol biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR023214 HAD-like domain
IPR007651 Lipin, N-terminal
IPR013209 LNS2, Lipin/Ned1/Smp2

UniProt Annotations

Entry Information

Gene Name
phosphatidate phosphatase PAH1
Protein Entry
PAH1_YEAST
UniProt ID
P32567
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity A 1,2-diacylglycerol 3-phosphate + H(2)O = a 1,2-diacyl-sn-glycerol + phosphate. {ECO:0000269|PubMed:16467296, ECO:0000269|PubMed:17910939, ECO:0000269|PubMed:17971454}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:16467296};
Domain Contains one Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity. {ECO:0000269|PubMed:20876142}.
Domain The N-terminal amphipathic helix (residues 1 to 18) is involved in the membrane recruitment. {ECO:0000269|PubMed:20876142}.
Enzyme Regulation Phenylglyoxal and propranolol inhibit activity in dose-dependent manners with IC(50) values of 1.3 mM and 0.2 mM, respectively. {ECO:0000269|PubMed:17910939}.
Function Mg(2+)-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. Required for de novo lipid synthesis and formation of lipid droplets. Controles transcription of phospholipid biosynthetic genes and nuclear structure by regulating the amount of membrane present at the nuclear envelope. Involved in plasmid maintenance, in respiration and in cell proliferation. {ECO:0000269|PubMed:15889145, ECO:0000269|PubMed:16467296, ECO:0000269|PubMed:16968695, ECO:0000269|PubMed:17910939, ECO:0000269|PubMed:17971454, ECO:0000269|PubMed:20876142, ECO:0000269|PubMed:21081492, ECO:0000269|PubMed:21422231, ECO:0000269|PubMed:8437575}.
Miscellaneous Present with 3910 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Ptm Phosphorylated by CDC28 at the onset of mitosis, and dephosphorylated by the NEM1-SPO7 complex. Phosphorylation regulates recruitment on promoters of lipid biosynthetic enzymes. {ECO:0000269|PubMed:15889145, ECO:0000269|PubMed:16968695, ECO:0000269|PubMed:17330950, ECO:0000269|PubMed:18407956, ECO:0000269|PubMed:19779198, ECO:0000269|PubMed:20876142, ECO:0000269|PubMed:21081492}.
Similarity Belongs to the lipin family. {ECO:0000305}.
Subcellular Location Cytoplasm. Nucleus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Identical and Related Proteins

Unique RefSeq proteins for LMP006918 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6323817 RefSeq NP_013888 862 phosphatidate phosphatase PAH1

Identical Sequences to LMP006918 proteins

Reference Database Accession Length Protein Name
GI:6323817 DBBJ BAA00880.1 862 Smp2 protein [Saccharomyces cerevisiae]
GI:6323817 EMBL CAA89801.1 862 Smp2p [Saccharomyces cerevisiae]
GI:6323817 GenBank AAV20367.1 862 Sequence 627 from patent US 6753314
GI:6323817 PRF - 862 SMP2 gene [Saccharomyces cerevisiae]
GI:6323817 SwissProt P32567.1 862 RecName: Full=Phosphatidic acid phosphohydrolase 1; Short=PAP1; AltName: Full=Protein SMP2 [Saccharomyces cerevisiae S288c]
GI:6323817 Third Party Genbank DAA10061.1 862 TPA: phosphatidate phosphatase PAH1 [Saccharomyces cerevisiae S288c]

Related Sequences to LMP006918 proteins

Reference Database Accession Length Protein Name
GI:6323817 DBBJ GAA25611.1 862 K7_Pah1p [Saccharomyces cerevisiae Kyokai no. 7]
GI:6323817 GenBank EDN64098.1 862 phosphatidate phosphohydrolase [Saccharomyces cerevisiae YJM789]
GI:6323817 GenBank EEU05667.1 862 Pah1p [Saccharomyces cerevisiae JAY291]
GI:6323817 GenBank EGA61043.1 862 Pah1p [Saccharomyces cerevisiae FostersO]
GI:6323817 GenBank AGM89448.1 864 Sequence 2 from patent US 8394614
GI:6323817 GenBank AGM89577.1 864 Sequence 2 from patent US 8394621