LIPID MAPS

LMPD ID

LMP006926

Gene ID

855764

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol alpha-1,6-mannosyltransferase

Gene Symbol

ALG12

Synonyms

ECM39

Chromosome

XIV

EC Number

2.4.1.260

dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol alpha-1,6-mannosyltransferase
Refseq ID	NP_014427
Protein GI	6324357
UniProt ID	P53730
mRNA ID	NM_001183207
Length	551
MRWSVLDTVLLTVISFHLIQAPFTKVEESFNIQAIHDILTYSVFDISQYDHLKFPGVVPRTFVGAVIIAMLSRPYLYLSSLIQTSRPTSIDVQLVVRGIVGLTNGLSFIYLKNCLQDMFDEITEKKKEENEDKDIYIYDSAGTWFLLFLIGSFHLMFYSTRTLPNFVMTLPLTNVALGWVLLGRYNAAIFLSALVAIVFRLEVSALSAGIALFSVIFKKISLFDAIKFGIFGLGLGSAISITVDSYFWQEWCLPEVDGFLFNVVAGYASKWGVEPVTAYFTHYLRMMFMPPTVLLLNYFGYKLAPAKLKIVSLASLFHIIVLSFQPHKEWRFIIYAVPSIMLLGATGAAHLWENMKVKKITNVLCLAILPLSIMTSFFISMAFLYISRMNYPGGEALTSFNDMIVEKNITNATVHISIPPCMTGVTLFGELNYGVYGINYDKTENTTLLQEMWPSFDFLITHEPTASQLPFENKTTNHWELVNTTKMFTGFDPTYIKNFVFQERVNVLSLLKQIIFDKTPTVFLKELTANSIVKSDVFFTYKRIKQDEKTD

Entrez Gene ID

855764

Gene Name

dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol alpha-1,6-mannosyltransferase

Gene Symbol

ALG12

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005783	IEA:UniProtKB-KW	C	endoplasmic reticulum
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0000009	IMP:SGD	F	alpha-1,6-mannosyltransferase activity
GO:0052917	IEA:UniProtKB-EC	F	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity
GO:0006488	IMP:SGD	P	dolichol-linked oligosaccharide biosynthetic process
GO:0097502	IMP:GOC	P	mannosylation
GO:0006486	IMP:SGD	P	protein glycosylation

KEGG Pathway Links

KEGG Pathway ID	Description
sce01100	Metabolic pathways
ko00510	N-Glycan biosynthesis
sce00510	N-Glycan biosynthesis
M00055	N-glycan precursor biosynthesis
sce_M00055	N-glycan precursor biosynthesis
ko00513	Various types of N-glycan biosynthesis
sce00513	Various types of N-glycan biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
MANNOSYL-CHITO-DOLICHOL-BIOSYNTHESIS	dolichyl-diphosphooligosaccharide biosynthesis
MANNOSYL-CHITO-DOLICHOL-BIOSYNTHESIS	dolichyl-diphosphooligosaccharide biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618350	Asparagine N-linked glycosylation
5618349	Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
5618074	Metabolism of proteins
5618285	Post-translational protein modification

InterPro Annotations

Accession	Description
IPR005599	GPI mannosyltransferase

Entry Information

Gene Name

dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol alpha-1,6-mannosyltransferase

Protein Entry

ALG12_YEAST

UniProt ID

P53730

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	Dolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D- GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha- (1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)- D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man- beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
Catalytic Activity	Dolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D- GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha- (1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)- D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man- beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269\|PubMed:15987956}.
Function	Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP- Man(7)GlcNAc(2)) required for protein glycosylation
Function	Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP- Man(7)GlcNAc(2)) required for protein glycosylation. {ECO:0000269\|PubMed:10336995, ECO:0000269\|PubMed:15987956}.
Interaction	P16474:KAR2; NbExp=1; IntAct=EBI-28496, EBI-7876;
Miscellaneous	Present with 3550 molecules/cell in log phase SD medium
Miscellaneous	Present with 3550 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Protein modification; protein glycosylation.
Similarity	Belongs to the glycosyltransferase 22 family
Similarity	Belongs to the glycosyltransferase 22 family. {ECO:0000305}.
Subcellular Location	Endoplasmic reticulum membrane ; Multi-pass membrane protein .
Subcellular Location	Endoplasmic reticulum membrane {ECO:0000269\|PubMed:14562095}; Multi-pass membrane protein {ECO:0000269\|PubMed:14562095}.