LIPID MAPS

LMPD ID

LMP006935

Gene ID

853732

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

phosphoglucomutase PGM1

Gene Symbol

PGM1

Synonyms

-

Chromosome

XI

EC Number

5.4.2.2 ; Glucose phosphomutase 1;

phosphoglucomutase PGM1
Refseq ID	NP_012795
Protein GI	6322722
UniProt ID	P33401
mRNA ID	NM_001179693
Length	570
MSLLIDSVPTVAYKDQKPGTSGLRKKTKVFMDEPHYTENFIQATMQSIPNGSEGTTLVVGGDGRFYNDVIMNKIAAVGAANGVRKLVIGQGGLLSTPAASHIIRTYEEKCTGGGIILTASHNPGGPENDLGIKYNLPNGGPAPESVTNAIWEASKKLTHYKIIKNFPKLNLNKLGKNQKYGPLLVDIIDPAKAYVQFLKEIFDFDLIKSFLAKQRKDKGWKLLFDSLNGITGPYGKAIFVDEFGLPAEEVLQNWHPLPDFGGLHPDPNLTYARTLVDRVDREKIAFGAASDGDGDRNMIYGYGPAFVSPGDSVAIIAEYAPEIPYFAKQGIYGLARSFPTSSAIDRVAAKKGLRCYEVPTGWKFFCALFDAKKLSICGEESFGTGSNHIREKDGLWAIIAWLNILAIYHRRNPEKEASIKTIQDEFWNEYGRTFFTRYDYEHIECEQAEKVVALLSEFVSRPNVCGSHFPADESLTVIDCGDFSYRDLDGSISENQGLFVKFSNGTKFVLRLSGTGSSGATIRLYVEKYTDKKENYGQTADVFLKPVINSIVKFLRFKEILGTDEPTVRT

Entrez Gene ID

853732

Gene Name

phosphoglucomutase PGM1

Gene Symbol

PGM1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005737	IDA:SGD	C	cytoplasm
GO:0000287	IEA:InterPro	F	magnesium ion binding
GO:0004614	IGI:SGD	F	phosphoglucomutase activity
GO:0006011	IGI:SGD	P	UDP-glucose metabolic process
GO:0019388	IGI:SGD	P	galactose catabolic process
GO:0019255	IGI:SGD	P	glucose 1-phosphate metabolic process
GO:0051156	ISS:SGD	P	glucose 6-phosphate metabolic process
GO:0006006	IEA:UniProtKB-KW	P	glucose metabolic process
GO:0005978	IGI:SGD	P	glycogen biosynthetic process
GO:0005992	IGI:SGD	P	trehalose biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
ko00520	Amino sugar and nucleotide sugar metabolism
sce00520	Amino sugar and nucleotide sugar metabolism
sce01110	Biosynthesis of secondary metabolites
ko00052	Galactose metabolism
sce00052	Galactose metabolism
ko00010	Glycolysis / Gluconeogenesis
sce00010	Glycolysis / Gluconeogenesis
sce01100	Metabolic pathways
M00549	Nucleotide sugar biosynthesis, glucose => UDP-glucose
sce_M00549	Nucleotide sugar biosynthesis, glucose => UDP-glucose
ko00030	Pentose phosphate pathway
sce00030	Pentose phosphate pathway
ko00230	Purine metabolism
sce00230	Purine metabolism
ko00500	Starch and sucrose metabolism
sce00500	Starch and sucrose metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-7343	UDP-glucose biosynthesis
PWY3O-1565	dolichyl glucosyl phosphate biosynthesis
GLYCOCAT-PWY	glycogen degradation

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618026	Disease
5618032	Galactose catabolism
5618021	Glucose metabolism
5618038	Glycogen breakdown (glycogenolysis)
5618025	Glycogen storage diseases
5618027	Glycogen synthesis
5618023	Metabolism
5618022	Metabolism of carbohydrates
5618024	Myoclonic epilepsy of Lafora

InterPro Annotations

Accession	Description
IPR005843	A-D-PHexomutase_C
IPR016066	A-D-PHexomutase_CS
IPR005844	A-D-PHexomutase_a/b/a-I
IPR016055	A-D-PHexomutase_a/b/a-I/II/III
IPR005845	A-D-PHexomutase_a/b/a-II
IPR005846	A-D-PHexomutase_a/b/a-III
IPR005841	Alpha-D-phosphohexomutase_SF

Entry Information

Gene Name

phosphoglucomutase PGM1

Protein Entry

PGM1_YEAST

UniProt ID

P33401

Species

Yeast (S288c)

Comments

Comment Type	Description
Biophysicochemical Properties	Kinetic parameters: KM=60 uM for alpha-D-glucose 1-phosphate {ECO:0000269\|PubMed:23103740}; Vmax=0.24 umol/min/mg enzyme for alpha-D-glucose 1-phosphate {ECO:0000269\|PubMed:23103740}; Vmax=0.06 umol/min/mg enzyme for D-ribose 1-phosphate {ECO:0000269\|PubMed:23103740};
Biophysicochemical Properties	Kinetic parameters: KM=60 uM for alpha-D-glucose 1-phosphate ; Vmax=0.24 umol/min/mg enzyme for alpha-D-glucose 1-phosphate ; Vmax=0.06 umol/min/mg enzyme for D-ribose 1-phosphate ;
Catalytic Activity	Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate
Catalytic Activity	Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate. {ECO:0000269\|PubMed:23103740}.
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250\|UniProtKB:P00949}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250\|UniProtKB:P00949};
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ; Note=Binds 1 Mg(2+) ion per subunit. ;
Function	Minor phosphoglucomutase isozyme that catalyzes the interconversion of glucose 1-phosphate and glucose 6-phosphate (PubMed:5784209). Constitutes about 10-20% of the phosphoglucomutase activity in the cell (PubMed:14264884, PubMed:5231755). Key enzyme in hexose metabolism. The forward reaction is an essential step in the energy metabolism of galactose since the product of the galactose pathway enzymes in yeast is glucose 1-phosphate. The reverse reaction is an essential step for biosynthesis when carbon sources other than galactose are the energy source because glucose 1-phosphate is the starting point for the synthesis of UDP-glucose, which acts as a precursor for the synthesis of oligosaccharides and trehalose (PubMed:14264884). {ECO:0000269\|PubMed:14264884, ECO:0000269\|PubMed:5231755, ECO:0000269\|PubMed:5784209}.
Miscellaneous	Present with 9820 molecules/cell in log phase SD medium
Miscellaneous	Present with 9820 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Belongs to the phosphohexose mutase family
Similarity	Belongs to the phosphohexose mutase family. {ECO:0000305}.
Subcellular Location	Cytoplasm .
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:14562095}.
Subunit	Monomer
Subunit	Monomer. {ECO:0000250\|UniProtKB:P37012}.