Gene/Proteome Database (LMPD)
Proteins
| Ste20p | |
|---|---|
| Refseq ID | NP_011856 |
| Protein GI | 6321780 |
| UniProt ID | Q03497 |
| mRNA ID | NM_001179087 |
| Length | 939 |
| RefSeq Status | PROVISIONAL |
| MSNDPSAVSELPDKDSLDNGISNDNERAMGGNGDGGDGLRLPRTTGTLNVNALQKGTNAAHEAGGYKSMDPAKNAETTNDDDNNVVSLDDPIQFTRVSSSSVISGMSSSMSPHSNIDETKSLEAVTPNINTSNITPDHSADNTFSTINASESDHQFNDTLLSKLSLTDSTETIENNATVKHQQPVASSTVNSNKSSTDIRRATPVSTPVISKPSMTTTPRQINSASHSLSNPKHKQHKPKVKPSKPEAKSKPVSVKKSFPSKNPLKNSSPPKKQTEKSYYSSSSKKRKSGSNSGTLRMKDVFTSFVQNIKRNSQDDKRASSSSNNSSSSSITTALRISTPYNAKHIHHVGVDSKTGEYTGLPEEWEKLLTSSGISKREQQQNMQAVMDIVKFYQDVTETNGEDKMFKTFNTTTGLPGSPQVSTPPANSFNKFPPSTSDSHNYGSRTGTPMSNHVMSPTLNTDSSSANGKFIPSRPAPKPPSSASASAPIIKSPVMNSAANVSPLKQTHAPTTPNRTSPNRSSISRNATLKKEEQPLPPIPPTKSKTSPIISTAHTPQQVAQSPKAPAQETVTTPTSKPAQARSLSKELNEKKREERERRKKQLYAKLNEICSDGDPSTKYANLVKIGQGASGGVYTAYEIGTNVSVAIKQMNLEKQPKKELIINEILVMKGSKHPNIVNFIDSYVLKGDLWVIMEYMEGGSLTDVVTHCILTEGQIGAVCRETLSGLEFLHSKGVLHRDIKSDNILLSMEGDIKLTDFGFCAQINELNLKRTTMVGTPYWMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPENLSSSLKKFLDWCLCVEPEDRASATELLHDEYITEIAEANSSLAPLVKLARLKKVAENMDADEDNDDDNDNEHINKTNNCDDNNDSKETVNLDVTEDDKQK | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:SGD | C | cytoplasm |
| GO:0000131 | IDA:SGD | C | incipient cellular bud site |
| GO:0043332 | IDA:SGD | C | mating projection tip |
| GO:0005634 | IDA:SGD | C | nucleus |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0008349 | IDA:SGD | F | MAP kinase kinase kinase kinase activity |
| GO:0044025 | IDA:SGD | F | histone kinase activity (H2B-S14 specific) |
| GO:0042802 | IPI:IntAct | F | identical protein binding |
| GO:0000165 | IDA:GOC | P | MAPK cascade |
| GO:0000185 | IDA:SGD | P | activation of MAPKKK activity |
| GO:0007121 | IMP:SGD | P | bipolar cellular bud site selection |
| GO:0007118 | IMP:SGD | P | budding cell apical bud growth |
| GO:0034605 | IMP:SGD | P | cellular response to heat |
| GO:0043991 | IDA:GOC | P | histone H2B-S14 phosphorylation |
| GO:0001403 | IMP:SGD | P | invasive growth in response to glucose limitation |
| GO:0010629 | IMP:SGD | P | negative regulation of gene expression |
| GO:0007232 | IMP:SGD | P | osmosensory signaling pathway via Sho1 osmosensor |
| GO:0000750 | IMP:SGD | P | pheromone-dependent signal transduction involved in conjugation with cellular fusion |
| GO:0043065 | IMP:SGD | P | positive regulation of apoptotic process |
| GO:0006468 | IDA:SGD | P | protein phosphorylation |
| GO:0007124 | IMP:SGD | P | pseudohyphal growth |
| GO:0007096 | IMP:SGD | P | regulation of exit from mitosis |
| GO:0001402 | IMP:SGD | P | signal transduction involved in filamentous growth |
| GO:0035376 | IMP:SGD | P | sterol import |
| GO:0034063 | IMP:SGD | P | stress granule assembly |
| GO:0000011 | IMP:SGD | P | vacuole inheritance |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| sce04011 | MAPK signaling pathway - yeast |
REACTOME Pathway Links
Domain Information
InterPro Annotations
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | ATP + a protein = ADP + a phosphoprotein. |
| Domain | The CRIB domain is required for the association with CDC42. |
| Function | MAP4K component of the MAPK pathway required for the mating pheromone response, haploid invasive growth and diploid pseudohyphal development. Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid cells, induction of a mating-specific gene FUS1, induction of mating-specific morphologies, and pheromone-induced proliferation arrest. Required for the regulation of the actin polarization and bud emergence during cell cycle in G1. Involved in the high osmolarity glycerol (HOG) response. Phosphorylates 'Thr-307' and 'Ser-302' or 'Ser-306' of STE11 and 'Ser-357' of MYO3. Phosphorylates histone H2B to form H2BS10ph during meiosis and H(2)O(2)-induced apoptosis. Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is required for the pheromone signaling. {ECO:0000269|PubMed:10562285, ECO:0000269|PubMed:10837245, ECO:0000269|PubMed:10970855, ECO:0000269|PubMed:11003652, ECO:0000269|PubMed:11940652, ECO:0000269|PubMed:12588977, ECO:0000269|PubMed:12686605, ECO:0000269|PubMed:1464311, ECO:0000269|PubMed:15652479, ECO:0000269|PubMed:15743816, ECO:0000269|PubMed:15970663, ECO:0000269|PubMed:7608157, ECO:0000269|PubMed:8421676, ECO:0000269|PubMed:8643578, ECO:0000269|PubMed:8722766, ECO:0000269|PubMed:9003780, ECO:0000269|PubMed:9009270, ECO:0000269|PubMed:9388196, ECO:0000269|PubMed:9428767, ECO:0000269|PubMed:9742399}. |
| Interaction | Self; NbExp=2; IntAct=EBI-18285, EBI-18285; P29366:BEM1; NbExp=11; IntAct=EBI-18285, EBI-3508; P19073:CDC42; NbExp=4; IntAct=EBI-18285, EBI-4274; P36006:MYO3; NbExp=3; IntAct=EBI-18285, EBI-11670; Q04439:MYO5; NbExp=4; IntAct=EBI-18285, EBI-11687; Q12163:NBP2; NbExp=5; IntAct=EBI-18285, EBI-34713; P80667:PEX13; NbExp=3; IntAct=EBI-18285, EBI-13206; P18851:STE4; NbExp=3; IntAct=EBI-18285, EBI-7390; |
| Miscellaneous | Present with 259 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Ptm | Autophosphorylated and phosphorylated by the CLN2-CDC28 complex in a cell cycle dependent manner. |
| Ptm | Autophosphorylated on serine residues. |
| Similarity | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. {ECO:0000305}. |
| Similarity | Contains 1 CRIB domain. {ECO:0000255|PROSITE- ProRule:PRU00057}. |
| Similarity | Contains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}. |
| Subcellular Location | Cytoplasm. Nucleus. Note=The translocation from the cytoplasm to the nucleus is stimulated by H(2)O(2). Localizes at bud emergence during cell cycle and the shmoo top during mating, both localizations requiring an interaction with CDC42. |
| Subunit | Interacts with BEM1, CDC42, CLN2, STE4 and the 14-3-3 proteins BMH1 and BMH2. {ECO:0000269|PubMed:10359756, ECO:0000269|PubMed:11003652, ECO:0000269|PubMed:11046150, ECO:0000269|PubMed:11940652, ECO:0000269|PubMed:12455995, ECO:0000269|PubMed:12586692, ECO:0000269|PubMed:15743816, ECO:0000269|PubMed:9003780, ECO:0000269|PubMed:9009270, ECO:0000269|PubMed:9215628, ECO:0000269|PubMed:9428767}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP006941 (as displayed in Record Overview)
Identical Sequences to LMP006941 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6321780 | GenBank | AAA35111.1 | 939 | protein kinase [Saccharomyces cerevisiae] |
| GI:6321780 | GenBank | AAB69747.1 | 939 | Ste20p: Protein kinase; component of the G-protein-coupled pheromone response pathway [Saccharomyces cerevisiae] |
| GI:6321780 | GenBank | ABC17134.1 | 939 | Sequence 380 from patent US 6949356 |
| GI:6321780 | GenBank | ABZ48081.1 | 939 | Sequence 22019 from patent US 7314974 |
| GI:6321780 | SwissProt | Q03497.1 | 939 | RecName: Full=Serine/threonine-protein kinase STE20 [Saccharomyces cerevisiae S288c] |
| GI:6321780 | Third Party Genbank | DAA06681.1 | 939 | TPA: Ste20p [Saccharomyces cerevisiae S288c] |
Related Sequences to LMP006941 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6321780 | DBBJ | GAA23703.1 | 939 | K7_Ste20p [Saccharomyces cerevisiae Kyokai no. 7] |
| GI:6321780 | EMBL | CAY80273.1 | 939 | Ste20p [Saccharomyces cerevisiae EC1118] |
| GI:6321780 | GenBank | AAA35038.1 | 939 | serine/threonine protein kinase [Saccharomyces cerevisiae] |
| GI:6321780 | GenBank | EDN62227.1 | 939 | PAK family kinase [Saccharomyces cerevisiae YJM789] |
| GI:6321780 | GenBank | EIW10138.1 | 939 | Ste20p [Saccharomyces cerevisiae CEN.PK113-7D] |
| GI:6321780 | GenBank | EWG85637.1 | 939 | Ste20p [Saccharomyces cerevisiae R008] |