LIPID MAPS

LMPD ID

LMP006942

Gene ID

855928

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Spt14p

Gene Symbol

SPT14

Synonyms

CWH6; GPI3

Chromosome

XVI

EC Number

2.4.1.198

Spt14p
Refseq ID	NP_015150
Protein GI	9755344
UniProt ID	P32363
mRNA ID	NM_001183989
Length	452
MGFNIAMLCDFFYPQLGGVEFHIYHLSQKLIDLGHSVVIITHAYKDRVGVRHLTNGLKVYHVPFFVIFRETTFPTVFSTFPIIRNILLREQIQIVHSHGSASTFAHEGILHANTMGLRTVFTDHSLYGFNNLTSIWVNKLLTFTLTNIDRVICVSNTCKENMIVRTELSPDIISVIPNAVVSEDFKPRDPTGGTKRKQSRDKIVIVVIGRLFPNKGSDLLTRIIPKVCSSHEDVEFIVAGDGPKFIDFQQMIESHRLQKRVQLLGSVPHEKVRDVLCQGDIYLHASLTEAFGTILVEAASCNLLIVTTQVGGIPEVLPNEMTVYAEQTSVSDLVQATNKAINIIRSKALDTSSFHDSVSKMYDWMDVAKRTVEIYTNISSTSSADDKDWMKMVANLYKRDGIWAKHLYLLCGIVEYMLFFLLEWLYPRDEIDLAPKWPKKTVSNETKEARET

Entrez Gene ID

855928

Gene Name

Spt14p

Gene Symbol

SPT14

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005783	IMP:SGD	C	endoplasmic reticulum
GO:0000506	TAS:SGD	C	glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0008194	ISS:SGD	F	UDP-glycosyltransferase activity
GO:0017176	IEA:UniProtKB-EC	F	phosphatidylinositol N-acetylglucosaminyltransferase activity
GO:0006506	ISS:SGD	P	GPI anchor biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
M00065	GPI-anchor biosynthesis, core oligosaccharide
sce_M00065	GPI-anchor biosynthesis, core oligosaccharide
ko00563	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
sce00563	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
sce01100	Metabolic pathways

InterPro Annotations

Accession	Description
IPR001296	Glycosyl transferase, family 1
IPR013234	PIGA, GPI anchor biosynthesis

Entry Information

Gene Name

Spt14p

Protein Entry

GPI3_YEAST

UniProt ID

P32363

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	UDP-N-acetyl-D-glucosamine + 1-phosphatidyl- 1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1- phosphatidyl-1D-myo-inositol.
Caution	Was originally thought to be involved in transcription
Caution	Was originally thought to be involved in transcription. {ECO:0000305\|PubMed:1660567}.
Enzyme Regulation	Inhibited by Ras, probably via the interaction between RAS2 and ERI1
Enzyme Regulation	Inhibited by Ras, probably via the interaction between RAS2 and ERI1. {ECO:0000269\|PubMed:15163411}.
Function	Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis
Function	Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis. {ECO:0000269\|PubMed:10970797, ECO:0000269\|PubMed:7768896}.
Miscellaneous	Present with 1480 molecules/cell in log phase SD medium
Miscellaneous	Present with 1480 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol- anchor biosynthesis.
Sequence Caution	Sequence=CAA97882.1; Type=Erroneous gene model prediction; Evidence= ;
Sequence Caution	Sequence=CAA97882.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity	Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily
Similarity	Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. {ECO:0000305}.
Subcellular Location	Endoplasmic reticulum membrane {ECO:0000305}; Single-pass membrane protein .
Subcellular Location	Endoplasmic reticulum membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Subunit	Component of the phosphatidylinositol N- acetylglucosaminyltransferase complex composed of at least GPI1, GPI2, GPI3, GPI15, GPI19 and ERI1.