Gene/Proteome Database (LMPD)

LMPD ID
LMP006967
Gene ID
850846
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
acetate--CoA ligase ACS2
Gene Symbol
ACS2
Synonyms
-
Chromosome
XII
EC Number
6.2.1.1

Proteins

acetate--CoA ligase ACS2
Refseq ID NP_013254
Protein GI 6323182
UniProt ID P52910
mRNA ID NM_001182040
Length 683
MTIKEHKVVYEAHNVKALKAPQHFYNSQPGKGYVTDMQHYQEMYQQSINEPEKFFDKMAKEYLHWDAPYTKVQSGSLNNGDVAWFLNGKLNASYNCVDRHAFANPDKPALIYEADDESDNKIITFGELLRKVSQIAGVLKSWGVKKGDTVAIYLPMIPEAVIAMLAVARIGAIHSVVFAGFSAGSLKDRVVDANSKVVITCDEGKRGGKTINTKKIVDEGLNGVDLVSRILVFQRTGTEGIPMKAGRDYWWHEEAAKQRTYLPPVSCDAEDPLFLLYTSGSTGSPKGVVHTTGGYLLGAALTTRYVFDIHPEDVLFTAGDVGWITGHTYALYGPLTLGTASIIFESTPAYPDYGRYWRIIQRHKATHFYVAPTALRLIKRVGEAEIAKYDTSSLRVLGSVGEPISPDLWEWYHEKVGNKNCVICDTMWQTESGSHLIAPLAGAVPTKPGSATVPFFGINACIIDPVTGVELEGNDVEGVLAVKSPWPSMARSVWNHHDRYMDTYLKPYPGHYFTGDGAGRDHDGYYWIRGRVDDVVNVSGHRLSTSEIEASISNHENVSEAAVVGIPDELTGQTVVAYVSLKDGYLQNNATEGDAEHITPDNLRRELILQVRGEIGPFASPKTIILVRDLPRTRSGKIMRRVLRKVASNEAEQLGDLTTLANPEVVPAIISAVENQFFSQKKK

Gene Information

Entrez Gene ID
850846
Gene Name
acetate--CoA ligase ACS2
Gene Symbol
ACS2
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:SGD C cytosol
GO:0005730 IDA:SGD C nucleolus
GO:0005634 IDA:SGD C nucleus
GO:0016208 IEA:InterPro F AMP binding
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0003987 IDA:SGD F acetate-CoA ligase activity
GO:0016880 IDA:SGD F acid-ammonia (or amide) ligase activity
GO:0006085 IDA:SGD P acetyl-CoA biosynthetic process
GO:0019427 IEA:InterPro P acetyl-CoA biosynthetic process from acetate
GO:0016573 IMP:SGD P histone acetylation
GO:0006090 IEA:UniProtKB-UniPathway P pyruvate metabolic process
GO:0001302 IMP:SGD P replicative cell aging

KEGG Pathway Links

KEGG Pathway ID Description
sce01110 Biosynthesis of secondary metabolites
ko01200 Carbon metabolism
sce01200 Carbon metabolism
ko00010 Glycolysis / Gluconeogenesis
sce00010 Glycolysis / Gluconeogenesis
sce01100 Metabolic pathways
ko00680 Methane metabolism
sce00680 Methane metabolism
ko00640 Propanoate metabolism
sce00640 Propanoate metabolism
ko00620 Pyruvate metabolism
sce00620 Pyruvate metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY0-1313 acetate conversion to acetyl-CoA
PWY0-1313 acetate conversion to acetyl-CoA
PWY66-21 ethanol degradation II
PWY66-162 ethanol degradation IV

REACTOME Pathway Links

REACTOME Pathway ID Description
5618031 Biological oxidations
5618063 Ethanol oxidation
5618023 Metabolism
5618064 Phase 1 - Functionalization of compounds

Domain Information

InterPro Annotations

Accession Description
IPR025110 AMP-binding enzyme C-terminal domain
IPR020845 AMP-binding, conserved site
IPR000873 AMP-dependent synthetase/ligase
IPR011904 Acetate-CoA ligase

UniProt Annotations

Entry Information

Gene Name
acetate--CoA ligase ACS2
Protein Entry
ACS2_YEAST
UniProt ID
P52910
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=8.8 mM for acetate {ECO:0000269|PubMed:8910545}; KM=1.3 mM for ATP {ECO:0000269|PubMed:8910545}; Vmax=0.34 umol/min/mg enzyme {ECO:0000269|PubMed:8910545};
Biophysicochemical Properties Kinetic parameters: KM=8.8 mM for acetate ; KM=1.3 mM for ATP ; Vmax=0.34 umol/min/mg enzyme ;
Catalytic Activity ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.
Function Catalyzes the production of acetyl-CoA. Provides the acetyl-CoA source for histone acetylation in the nucleus. "Anaerobic" isozyme of acetyl-coenzyme A synthetase, which is required for growth on fermentable carbon sources such as glucose. May be involved in the PDH (pyruvate dehydrogenase complex) bypass
Function Catalyzes the production of acetyl-CoA. Provides the acetyl-CoA source for histone acetylation in the nucleus. "Anaerobic" isozyme of acetyl-coenzyme A synthetase, which is required for growth on fermentable carbon sources such as glucose. May be involved in the PDH (pyruvate dehydrogenase complex) bypass. {ECO:0000269|PubMed:16857587, ECO:0000269|PubMed:8910545}.
Miscellaneous Present with 225000 molecules/cell in log phase SD medium
Miscellaneous Present with 225000 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Carbohydrate metabolism; pyruvate metabolism.
Similarity Belongs to the ATP-dependent AMP-binding enzyme family
Similarity Belongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular Location Cytoplasm. Nucleus. Note=Predominantly nuclear.

Identical and Related Proteins

Unique RefSeq proteins for LMP006967 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6323182 RefSeq NP_013254 683 acetate--CoA ligase ACS2

Identical Sequences to LMP006967 proteins

Reference Database Accession Length Protein Name

Related Sequences to LMP006967 proteins

Reference Database Accession Length Protein Name