Gene/Proteome Database (LMPD)

LMPD ID
LMP006999
Gene ID
852008
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
hypoxanthine phosphoribosyltransferase
Gene Symbol
HPT1
Synonyms
BRA6
Alternate Names
hypoxanthine phosphoribosyltransferase
Chromosome
IV
EC Number
2.4.2.8

Proteins

hypoxanthine phosphoribosyltransferase
Refseq ID NP_010687
Protein GI 398366563
UniProt ID Q04178
mRNA ID NM_001180707
Length 221
RefSeq Status PROVISIONAL
MSANDKQYISYNNVHQLCQVSAERIKNFKPDLIIAIGGGGFIPARILRTFLKEPGVPTIRIFAIILSLYEDLNSVGSEVEEVGVKVSRTQWIDYEQCKLDLVGKNVLIVDEVDDTRTTLHYALSELEKDAAEQAKAKGIDTEKSPEMKTNFGIFVLHDKQKPKKADLPAEMLNDKNRYFAAKTVPDKWYAYPWESTDIVFHTRMAIEQGNDIFIPEQEHKQ

Gene Information

Entrez Gene ID
852008
Gene Name
hypoxanthine phosphoribosyltransferase
Gene Symbol
HPT1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0052657 IEA:UniProtKB-EC F guanine phosphoribosyltransferase activity
GO:0004422 IDA:SGD F hypoxanthine phosphoribosyltransferase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0000166 IEA:UniProtKB-KW F nucleotide binding
GO:0032263 IDA:SGD P GMP salvage
GO:0032264 IDA:SGD P IMP salvage

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6610 adenine and adenosine salvage IV
PWY3O-2220 salvage pathways of adenine, hypoxanthine and their nucleosides
PWY3O-743 salvage pathways of guanine, xanthine and their nucleosides
PWY3O-1 superpathway of purine nucleosides salvage

Domain Information

InterPro Annotations

Accession Description
IPR000836 Phosphoribosyltransferase domain
IPR029057 Phosphoribosyltransferase-like

UniProt Annotations

Entry Information

Gene Name
hypoxanthine phosphoribosyltransferase
Protein Entry
HPRT_YEAST
UniProt ID
Q04178
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=23 uM for hypoxanthine {ECO:0000269|PubMed:371963, ECO:0000269|PubMed:6170313, ECO:0000269|PubMed:7035445}; KM=18 uM for guanine {ECO:0000269|PubMed:371963, ECO:0000269|PubMed:6170313, ECO:0000269|PubMed:7035445}; KM=50 uM for phosphoribosylpyrophosphate {ECO:0000269|PubMed:371963, ECO:0000269|PubMed:6170313, ECO:0000269|PubMed:7035445}; pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:371963, ECO:0000269|PubMed:6170313, ECO:0000269|PubMed:7035445};
Catalytic Activity GMP + diphosphate = guanine + 5-phospho-alpha- D-ribose 1-diphosphate. {ECO:0000269|PubMed:18245832}.
Catalytic Activity IMP + diphosphate = hypoxanthine + 5-phospho- alpha-D-ribose 1-diphosphate. {ECO:0000269|PubMed:18245832}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.;
Enzyme Regulation Subject to feedback inhibition by GMP. {ECO:0000269|PubMed:18245832}.
Function Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. {ECO:0000269|PubMed:11035032, ECO:0000269|PubMed:18245832, ECO:0000269|PubMed:371963, ECO:0000269|PubMed:6170313, ECO:0000269|PubMed:6392474, ECO:0000269|PubMed:7035445, ECO:0000269|PubMed:9335580}.
Miscellaneous Present with 36500 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.
Similarity Belongs to the purine/pyrimidine phosphoribosyltransferase family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}.
Subunit Dimer. {ECO:0000269|PubMed:18245832, ECO:0000269|PubMed:6170313}.

Identical and Related Proteins

Unique RefSeq proteins for LMP006999 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
398366563 RefSeq NP_010687 221 hypoxanthine phosphoribosyltransferase

Identical Sequences to LMP006999 proteins

Reference Database Accession Length Protein Name
GI:398366563 GenBank EHN07694.1 221 Hpt1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:398366563 GenBank EIW11609.1 221 Hpt1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:398366563 GenBank EWG86737.1 221 Hpt1p [Saccharomyces cerevisiae R008]
GI:398366563 GenBank EWG92052.1 221 Hpt1p [Saccharomyces cerevisiae P301]
GI:398366563 GenBank EWG96683.1 221 Hpt1p [Saccharomyces cerevisiae R103]
GI:398366563 GenBank EWH19092.1 221 Hpt1p [Saccharomyces cerevisiae P283]

Related Sequences to LMP006999 proteins

Reference Database Accession Length Protein Name
GI:398366563 EMBL CAY78902.1 221 Hpt1p [Saccharomyces cerevisiae EC1118]
GI:398366563 gnl McCuskerlabDuke 221 Hpt1p [Saccharomyces cerevisiae YJM993]
GI:398366563 PDB 2JKZ 220 Chain A, Saccharomyces Cerevisiae Hypoxanthine-Guanine Phosphoribosyltransferase In Complex With Gmp (Guanosine 5' -Monophosphate) (Orthorhombic Crystal Form)
GI:398366563 PDB 2JKZ 220 Chain B, Saccharomyces Cerevisiae Hypoxanthine-Guanine Phosphoribosyltransferase In Complex With Gmp (Guanosine 5' -Monophosphate) (Orthorhombic Crystal Form)
GI:398366563 PDB 2JKZ 220 Chain C, Saccharomyces Cerevisiae Hypoxanthine-Guanine Phosphoribosyltransferase In Complex With Gmp (Guanosine 5' -Monophosphate) (Orthorhombic Crystal Form)
GI:398366563 PDB 2JKZ 220 Chain D, Saccharomyces Cerevisiae Hypoxanthine-Guanine Phosphoribosyltransferase In Complex With Gmp (Guanosine 5' -Monophosphate) (Orthorhombic Crystal Form)