LIPID MAPS

LMPD ID

LMP007037

Gene ID

855630

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

phosphatidate phosphatase APP1

Gene Symbol

APP1

Synonyms

-

Chromosome

XIV

EC Number

3.1.3.4

phosphatidate phosphatase APP1
Refseq ID	NP_014305
Protein GI	6324235
UniProt ID	P53933
mRNA ID	NM_001182932
Length	587
MNSQGYDESSSSTAATSGPTSGDPRMGKKQRFMNLIRTTKDVYIPNLTSSISQKTMDGIRSTTNSFEGYNDLPMELPHNTTITYFPTYTTTNLVDPDGLSAPRKDFETTVRCAVSYPGNPTSRRNRWLLSLCKQYLRTGTAEADVAPVVPPHLEEDSGDLNDSQSSIESSLSSKSENRYSHMGIQEEDVLNERIQGFLSKKVPNTPVVVDLLPKDKLRGDTASFFGTTDSYGNLLIKAETDFLPSKINITLDTPIEGHADPISETFPANYVSPYGIGLISDIDDTIKHTGVTGDRRSMFRNVFIHDVQSWVIDGVPLWYKTLHDVADVDFFYVSNSPIQTFTLLKQYICANFPPGPIFLKQYSGNFFSTIMTSSANRKIQPIANILKDFPKKKFILVGDSGEHDLEAYTTTALQFPNQILAIYIRCCSNSMSDVPSHDEEVMNEVNNIIELQQRPMQMTKSTVRTRRRPPPPPIPSTQKPSLTEEQTESIRMSRRNKDENNAKRVAPPPLPNRQLPNLDANTYYVPSSQNDYGMYGAFMDKKADEWKRRVMDSIQKLSNQDTTLMFFSDPALSLEDSIRRIREKYSN

Entrez Gene ID

855630

Gene Name

phosphatidate phosphatase APP1

Gene Symbol

APP1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0030479	IDA:SGD	C	actin cortical patch
GO:0015629	IPI:SGD	C	actin cytoskeleton
GO:0008195	IDA:SGD	F	phosphatidate phosphatase activity
GO:0030036	IPI:SGD	P	actin cytoskeleton organization
GO:0044255	IMP:SGD	P	cellular lipid metabolic process
GO:0016311	IDA:GOC	P	dephosphorylation

InterPro Annotations

Accession	Description
IPR019236	DUF2183
IPR017210	UCP037464_APP1

Entry Information

Gene Name

phosphatidate phosphatase APP1

Protein Entry

APP1_YEAST

UniProt ID

P53933

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	A 1,2-diacylglycerol 3-phosphate + H(2)O = a 1,2-diacyl-sn-glycerol + phosphate
Catalytic Activity	A 1,2-diacylglycerol 3-phosphate + H(2)O = a 1,2-diacyl-sn-glycerol + phosphate. {ECO:0000269\|PubMed:23071111}.
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ;
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Domain	Contains one Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
Enzyme Regulation	Inhibited by N-ethylmaleimide
Enzyme Regulation	Inhibited by N-ethylmaleimide. {ECO:0000269\|PubMed:23071111}.
Function	Mg(2+)-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. May play a role in vesicular trafficking through its PAP activity at cortical actin patches
Function	Mg(2+)-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. May play a role in vesicular trafficking through its PAP activity at cortical actin patches. {ECO:0000269\|PubMed:23071111}.
Interaction	P15891:ABP1; NbExp=9; IntAct=EBI-28798, EBI-2036; P47068:BBC1; NbExp=3; IntAct=EBI-28798, EBI-3437; P38822:BZZ1; NbExp=14; IntAct=EBI-28798, EBI-3889; Q05080:HOF1; NbExp=2; IntAct=EBI-28798, EBI-5412; P53281:LSB1; NbExp=4; IntAct=EBI-28798, EBI-23329; P43603:LSB3; NbExp=8; IntAct=EBI-28798, EBI-22980; Q04439:MYO5; NbExp=2; IntAct=EBI-28798, EBI-11687; P80667:PEX13; NbExp=2; IntAct=EBI-28798, EBI-13206; Q06449:PIN3; NbExp=4; IntAct=EBI-28798, EBI-35523; P39743:RVS167; NbExp=9; IntAct=EBI-28798, EBI-14500; P32790:SLA1; NbExp=4; IntAct=EBI-28798, EBI-17313; P32793:YSC84; NbExp=6; IntAct=EBI-28798, EBI-24460;
Miscellaneous	Present with 2289 molecules/cell in log phase SD medium
Miscellaneous	Present with 2289 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Ptm	N-glycosylated
Ptm	N-glycosylated. {ECO:0000269\|PubMed:19756047}.
Sequence Caution	Sequence=CAA59823.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence= ;
Sequence Caution	Sequence=CAA59823.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Subcellular Location	Cytoplasm, cytoskeleton, actin patch .
Subcellular Location	Cytoplasm, cytoskeleton, actin patch {ECO:0000269\|PubMed:11489916, ECO:0000269\|PubMed:14562095}.
Subunit	Interacts with ABP1
Subunit	Interacts with ABP1. {ECO:0000269\|PubMed:14737190}.