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Comment Type
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Description
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Biophysicochemical Properties
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Kinetic parameters: KM=2.24 uM for alpha-D-glucose 1,6-diphosphate {ECO:0000269|PubMed:1100398}; KM=23.4 uM for alpha-D-glucose 1-phosphate {ECO:0000269|PubMed:1100398}; KM=26 uM for alpha-D-glucose 1-phosphate {ECO:0000269|PubMed:23103740}; KM=530 uM for D-ribose 1-phosphate {ECO:0000269|PubMed:23103740}; Vmax=33.7 umol/min/mg enzyme for alpha-D-glucose 1-phosphate {ECO:0000269|PubMed:23103740}; Vmax=0.32 umol/min/mg enzyme for D-ribose 1-phosphate {ECO:0000269|PubMed:23103740};
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Biophysicochemical Properties
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Kinetic parameters: KM=2.24 uM for alpha-D-glucose 1,6-diphosphate ; KM=23.4 uM for alpha-D-glucose 1-phosphate ; KM=26 uM for alpha-D-glucose 1-phosphate ; KM=530 uM for D-ribose 1-phosphate ; Vmax=33.7 umol/min/mg enzyme for alpha-D-glucose 1-phosphate ; Vmax=0.32 umol/min/mg enzyme for D-ribose 1-phosphate ;
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Catalytic Activity
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Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate. {ECO:0000269|PubMed:1100398, ECO:0000269|PubMed:23103740, ECO:0000269|PubMed:4992300}.
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Cofactor
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Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:1100398, ECO:0000269|PubMed:4628805}; Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:1100398, ECO:0000269|PubMed:4628805}; Note=Binds 1 magnesium ion per subunit. Can also use Zn(2+) as cofactor. {ECO:0000269|PubMed:1100398, ECO:0000269|PubMed:4628805};
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Cofactor
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Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:1100398, ECO:0000269|PubMed:4628805}; Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:1100398, ECO:0000269|PubMed:4628805}; Note=Binds 1 magnesium ion per subunit. Can also use Zn(2+) as cofactor. {ECO:0000269|PubMed:1100398, ECO:0000269|PubMed:4628805};
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Disruption Phenotype
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Blocks galactose utilization, but does not impair growth on glucose
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Disruption Phenotype
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Blocks galactose utilization, but does not impair growth on glucose. {ECO:0000269|PubMed:14264884}.
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Function
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Major phosphoglucomutase isozyme that catalyzes the reversible interconversion of glucose 1-phosphate and glucose 6- phosphate (PubMed:5784209). Constitutes about 80-90% of the phosphoglucomutase activity in the cell (PubMed:14264884, PubMed:5231755). Key enzyme in hexose metabolism. The forward reaction is an essential step in the energy metabolism of galactose since the product of the galactose pathway enzymes in yeast is glucose 1-phosphate. The reverse reaction is an essential step for biosynthesis when carbon sources other than galactose are the energy source because glucose 1-phosphate is the starting point for the synthesis of UDP-glucose, which acts as a precursor for the synthesis of oligosaccharides and trehalose (PubMed:14264884). {ECO:0000269|PubMed:14264884, ECO:0000269|PubMed:5231755}.
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Induction
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Induced in response to galactose and severely repressed in response to glucose
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Induction
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Induced in response to galactose and severely repressed in response to glucose. {ECO:0000269|PubMed:2138705}.
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Miscellaneous
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Present with 3790 molecules/cell in log phase SD medium
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Miscellaneous
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Present with 3790 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
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Ptm
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O-glycosylated with mannose residues (By similarity). Substrate of UDP-glucose--glycoprotein glucose phosphotransferase, linking glucose in a phosphodiester linkage to O-linked mannose (PubMed:8385141, PubMed:7929458). {ECO:0000250|UniProtKB:P47244, ECO:0000269|PubMed:7929458, ECO:0000269|PubMed:8385141}.
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Similarity
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Belongs to the phosphohexose mutase family
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Similarity
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Belongs to the phosphohexose mutase family. {ECO:0000305}.
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Subcellular Location
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Cytoplasm .
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Subcellular Location
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Cytoplasm {ECO:0000269|PubMed:8385141}.
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Subunit
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Monomer
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Subunit
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Monomer. {ECO:0000269|PubMed:1100398}.
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