Gene/Proteome Database (LMPD)

LMPD ID
LMP007050
Gene ID
856523
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase
Gene Symbol
EPT1
Synonyms
-
Alternate Names
bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase
Chromosome
VIII
EC Number
2.7.8.1

Proteins

bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase
Refseq ID NP_011991
Protein GI 6321915
UniProt ID P22140
mRNA ID NM_001179253
Length 391
RefSeq Status PROVISIONAL
MGYFVPDSHIENLKSYKYQSEDRSLVSKYFLKPFWQRFCHIFPTWMAPNIITLSGFAFIVINVLTVFYYDPNLNTDTPRWTYFSYALGVFLYQTFDGCDGVHARRINQSGPLGELFDHSIDAINSTLSIFIFASETGMGFSYNLMLSQFAMLTNFYLSTWEEYHTHTLYLSEFSGPVEGILIVCVSLILTGIYGKQVIWHTYLFTITVGDKVIDVDTLDIVFSLAVFGLVMNALSAKRNVDKYYRNSTSSANNITQIEQDSAIKGLLPFFAYYASIALLVWMQPSFITLSFILSVGFTGAFTVGRIIVCHLTKQSFPMFNAPMLIPLCQIVLYKICLSLWGIESNKIVFALSWLGFGLSLGVHIMFMNDIIHEFTEYLDVYALSIKRSKLT

Gene Information

Entrez Gene ID
856523
Gene Name
bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase
Gene Symbol
EPT1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0004142 IDA:SGD F diacylglycerol cholinephosphotransferase activity
GO:0004307 IDA:SGD F ethanolaminephosphotransferase activity
GO:0006657 IDA:GOC P CDP-choline pathway
GO:0006656 IDA:SGD P phosphatidylcholine biosynthetic process
GO:0006646 IDA:SGD P phosphatidylethanolamine biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
sce00565 Ether lipid metabolism
sce00564 Glycerophospholipid metabolism
sce_M00092 Phosphatidylethanolamine (PE) biosynthesis, ethanolamine => PE

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-7367 phosphatidylcholine resynthesis via glycerophosphocholine

Domain Information

InterPro Annotations

Accession Description
IPR000462 CDP-alcohol phosphatidyltransferase
IPR014472 Choline/ethanolamine phosphotransferase

UniProt Annotations

Entry Information

Gene Name
bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase
Protein Entry
EPT1_YEAST
UniProt ID
P22140
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=120 uM for CDP-choline {ECO:0000269|PubMed:1847919}; KM=29 uM for CDP-dimethylethanolamine {ECO:0000269|PubMed:1847919}; KM=29 uM for CDP-monomethylethanolamine {ECO:0000269|PubMed:1847919}; KM=22 uM for CDP-ethanolamine {ECO:0000269|PubMed:1847919}; Vmax=0.62 nmol/min/mg enzyme for CDP-choline {ECO:0000269|PubMed:1847919}; Vmax=0.42 nmol/min/mg enzyme for CDP-dimethylethanolamine {ECO:0000269|PubMed:1847919}; Vmax=0.27 nmol/min/mg enzyme for CDP-monomethylethanolamine {ECO:0000269|PubMed:1847919}; Vmax=1.35 nmol/min/mg enzyme for CDP-ehylethanolamine {ECO:0000269|PubMed:1847919};
Catalytic Activity CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.
Catalytic Activity CDP-ethanolamine + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylethanolamine.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:1847919};
Enzyme Regulation Requires a divalent cation activator, and is inhibited by CMP. Activated by phospholipids, especially phosphatidylcholine. {ECO:0000269|PubMed:1847919}.
Function Catalyzes the final step in the CDP-ethanolamine route leading to phosphatidylethanolamine (PE). Can also catalyze the formation of phosphatidylcholine (PC) from CDP-choline, but does not substantially contribute to PC biosynthesis. Preferentially uses CDP-dimethylethanolamine and CDP-propanolamine as aminoalcohol substrates. Shows highest activity toward di- unsaturated diacylglycerol species as lipid substrates. The CDP- ethanolamine pathway may play a role in maintaining the proper PE species distribution. {ECO:0000269|PubMed:15225629, ECO:0000269|PubMed:1847919, ECO:0000269|PubMed:2848840, ECO:0000269|PubMed:7961445, ECO:0000269|PubMed:7961735}.
Induction Repressed by inositol. Repression is dependent on the presence of CPT1. {ECO:0000269|PubMed:7961831}.
Pathway Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.
Pathway Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 3/3.
Similarity Belongs to the CDP-alcohol phosphatidyltransferase class-I family. {ECO:0000305}.
Subcellular Location Golgi apparatus membrane; Multi-pass membrane protein.

Identical and Related Proteins

Unique RefSeq proteins for LMP007050 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6321915 RefSeq NP_011991 391 bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase

Identical Sequences to LMP007050 proteins

Reference Database Accession Length Protein Name
GI:6321915 GenBank EIW10025.1 391 Ept1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6321915 GenBank EWG85767.1 391 Ept1p [Saccharomyces cerevisiae R008]
GI:6321915 GenBank EWG90699.1 391 Ept1p [Saccharomyces cerevisiae P301]
GI:6321915 GenBank EWG95568.1 391 Ept1p [Saccharomyces cerevisiae R103]
GI:6321915 gnl McCuskerlabDuke 391 Ept1p [Saccharomyces cerevisiae YJM993]
GI:6321915 Third Party Genbank DAA06817.1 391 TPA: bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007050 proteins

Reference Database Accession Length Protein Name
GI:6321915 DBBJ GAA23840.1 391 K7_Ept1p [Saccharomyces cerevisiae Kyokai no. 7]
GI:6321915 EMBL CAY80132.1 391 Ept1p [Saccharomyces cerevisiae EC1118]
GI:6321915 GenBank AAA63572.1 391 sn-1,2-diacylglycerol ehtanolamine phosphotransferase [Saccharomyces cerevisiae]
GI:6321915 GenBank EDN62362.1 391 diacylglycerol cholinephosphotransferase [Saccharomyces cerevisiae YJM789]
GI:6321915 GenBank EGA61989.1 346 Ept1p [Saccharomyces cerevisiae FostersO]
GI:6321915 GenBank EWH18149.1 391 Ept1p [Saccharomyces cerevisiae P283]