Gene/Proteome Database (LMPD)

LMPD ID
LMP007070
Gene ID
852530
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
glucan 1,3-alpha-glucosidase ROT2
Gene Symbol
ROT2
Synonyms
GLS2
Chromosome
II
EC Number
3.2.1.84

Proteins

glucan 1,3-alpha-glucosidase ROT2
Refseq ID NP_009788
Protein GI 398365611
UniProt ID P38138
mRNA ID NM_001178577
Length 954
MVLLKWLVCQLVFFTAFSHAFTDYLLKKCAQSGFCHRNRVYAENIAKSHHCYYKVDAESIAHDPLENVLHATIIKTIPRLEGDDIAVQFPFSLSFLQDHSVRFTINEKERMPTNSSGLLISSQRFNETWKYAFDKKFQEEANRTSIPQFHFLKQKQTVNSFWSKISSFLSLSNSTADTFHLRNGDVSVEIFAEPFQLKVYWQNALKLIVNEQNFLNIEHHRTKQENFAHVLPEETTFNMFKDNFLYSKHDSMPLGPESVALDFSFMGSTNVYGIPEHATSLRLMDTSGGKEPYRLFNVDVFEYNIGTSQPMYGSIPFMFSSSSTSIFWVNAADTWVDIKYDTSKNKTMTHWISENGVIDVVMSLGPDIPTIIDKFTDLTGRPFLPPISSIGYHQCRWNYNDEMDVLTVDSQMDAHMIPYDFIWLDLEYTNDKKYFTWKQHSFPNPKRLLSKLKKLGRNLVVLIDPHLKKDYEISDRVINENVAVKDHNGNDYVGHCWPGNSIWIDTISKYGQKIWKSFFERFMDLPADLTNLFIWNDMNEPSIFDGPETTAPKDLIHDNYIEERSVHNIYGLSVHEATYDAIKSIYSPSDKRPFLLTRAFFAGSQRTAATWTGDNVANWDYLKISIPMVLSNNIAGMPFIGADIAGFAEDPTPELIARWYQAGLWYPFFRAHAHIDTKRREPYLFNEPLKSIVRDIIQLRYFLLPTLYTMFHKSSVTGFPIMNPMFIEHPEFAELYHIDNQFYWSNSGLLVKPVTEPGQSETEMVFPPGIFYEFASLHSFINNGTDLIEKNISAPLDKIPLFIEGGHIITMKDKYRRSSMLMKNDPYVIVIAPDTEGRAVGDLYVDDGETFGYQRGEYVETQFIFENNTLKNVRSHIPENLTGIHHNTLRNTNIEKIIIAKNNLQHNITLKDSIKVKKNGEESSLPTRSSYENDNKITILNLSLDITEDWEVIF

Gene Information

Entrez Gene ID
852530
Gene Name
glucan 1,3-alpha-glucosidase ROT2
Gene Symbol
ROT2
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 ISS:SGD C endoplasmic reticulum
GO:0005788 IDA:SGD C endoplasmic reticulum lumen
GO:0017177 IPI:SGD C glucosidase II complex
GO:0030246 IEA:InterPro F carbohydrate binding
GO:0033919 IMP:SGD F glucan 1,3-alpha-glucosidase activity
GO:0005975 IEA:InterPro P carbohydrate metabolic process
GO:0009272 IMP:SGD P fungal-type cell wall biogenesis

KEGG Pathway Links

KEGG Pathway ID Description
sce01100 Metabolic pathways
ko00510 N-Glycan biosynthesis
sce00510 N-Glycan biosynthesis
M00074 N-glycan biosynthesis, high-mannose type
sce_M00074 N-glycan biosynthesis, high-mannose type
M00073 N-glycan precursor trimming
sce_M00073 N-glycan precursor trimming
ko04141 Protein processing in endoplasmic reticulum
sce04141 Protein processing in endoplasmic reticulum

Domain Information

InterPro Annotations

Accession Description
IPR011013 Gal_mutarotase_SF_dom
IPR000322 Glyco_hydro_31
IPR025887 Glyco_hydro_31_N_dom
IPR017853 Glycoside hydrolase superfamily

UniProt Annotations

Entry Information

Gene Name
glucan 1,3-alpha-glucosidase ROT2
Protein Entry
GLU2A_YEAST
UniProt ID
P38138
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Optimum pH is 5.8-6.8. {ECO:0000269|PubMed:6216253};
Biophysicochemical Properties pH dependence: Optimum pH is 5.8-6.8. ;
Catalytic Activity Hydrolysis of terminal (1->3)-alpha-D- glucosidic links in (1->3)-alpha-D-glucans.
Enzyme Regulation Inhibited by glucose, maltose and nigerose, and by the antibiotic deoxynojirimycin.
Function Catalytic subunit of glucosidase 2, which cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. {ECO:0000269|PubMed:16373354, ECO:0000269|PubMed:8910335}.
Interaction Q04924:GTB1; NbExp=1; IntAct=EBI-21021, EBI-37493;
Miscellaneous Present with 238 molecules/cell in log phase SD medium
Miscellaneous Present with 238 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Glycan metabolism; N-glycan metabolism.
Similarity Belongs to the glycosyl hydrolase 31 family
Similarity Belongs to the glycosyl hydrolase 31 family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum .
Subcellular Location Endoplasmic reticulum {ECO:0000269|PubMed:16373354}.
Subunit Heterodimer of a catalytic subunit alpha (ROT2) and a subunit beta (GTB1).

Identical and Related Proteins

Unique RefSeq proteins for LMP007070 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
398365611 RefSeq NP_009788 954 glucan 1,3-alpha-glucosidase ROT2

Identical Sequences to LMP007070 proteins

Reference Database Accession Length Protein Name

Related Sequences to LMP007070 proteins

Reference Database Accession Length Protein Name