Gene/Proteome Database (LMPD)

LMPD ID
LMP007087
Gene ID
854334
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
polyphosphatase DDP1
Gene Symbol
DDP1
Synonyms
-
Alternate Names
polyphosphatase DDP1
Chromosome
XV
EC Number
3.6.1.52

Proteins

polyphosphatase DDP1
Refseq ID NP_014806
Protein GI 6324737
UniProt ID Q99321
mRNA ID NM_001183582
Length 188
RefSeq Status PROVISIONAL
MGKTADNHGPVRSETAREGRENQVYSPVTGARLVAGCICLTPDKKQVLMITSSAHKKRWIVPKGGVEKDEPNYETTAQRETWEEAGCIGKIVANLGTVEDMRPPKDWNKDIKQFENSRKDSEVAKHPPRTEFHFYELEIENLLDKFPECHKRHRKLYSYTEAKQNLIDAKRPELLEALNRSAIIKDDK

Gene Information

Entrez Gene ID
854334
Gene Name
polyphosphatase DDP1
Gene Symbol
DDP1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0034431 IDA:SGD F bis(5'-adenosyl)-hexaphosphatase activity
GO:0034432 IDA:SGD F bis(5'-adenosyl)-pentaphosphatase activity
GO:0008486 IDA:SGD F diphosphoinositol-polyphosphate diphosphatase activity
GO:0000298 IDA:SGD F endopolyphosphatase activity
GO:0052846 IEA:UniProtKB-EC F inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity
GO:0052847 IEA:UniProtKB-EC F inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity
GO:0052843 IEA:UniProtKB-EC F inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity
GO:0052848 IEA:UniProtKB-EC F inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity
GO:0052844 IEA:UniProtKB-EC F inositol-3-diphosphate-1,2,4,5,6-pentakisphosphate diphosphatase activity
GO:0052845 IEA:UniProtKB-EC F inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity
GO:0052840 IEA:UniProtKB-EC F inositol diphosphate tetrakisphosphate diphosphatase activity
GO:0050072 IDA:SGD F m7G(5')pppN diphosphatase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:1990174 IDA:SGD F phosphodiesterase decapping endonuclease activity
GO:0015961 IDA:SGD P diadenosine polyphosphate catabolic process
GO:0046855 IDA:SGD P inositol phosphate dephosphorylation
GO:0006798 IDA:SGD P polyphosphate catabolic process
GO:0090502 IDA:GOC P RNA phosphodiester bond hydrolysis, endonucleolytic

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY3O-402 inositol phosphate biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR000086 NUDIX hydrolase domain
IPR015797 NUDIX hydrolase domain-like

UniProt Annotations

Entry Information

Gene Name
polyphosphatase DDP1
Protein Entry
DDP1_YEAST
UniProt ID
Q99321
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=31 nM for PP-InsP5 {ECO:0000269|PubMed:10419486};
Catalytic Activity Diphospho-myo-inositol polyphosphate + H(2)O = myo-inositol polyphosphate + phosphate. {ECO:0000269|PubMed:10419486}.
Catalytic Activity P(1),P(5)-bis(5'-adenosyl)pentaphosphate + H(2)O = adenosine 5'-tetraphosphate + AMP. {ECO:0000269|PubMed:10419486}.
Catalytic Activity P(1),P(6)-bis(5'-adenosyl)hexaphosphate + H(2)O = adenosine 5'-pentaphosphate + AMP. {ECO:0000269|PubMed:10419486}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Function May eliminate potentially toxic dinucleoside polyphosphates during sporulation. Most active against diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A). Can also hydrolyze diadenosine 5',5'''-P1,P5-pentaphosphate (Ap5A), adenosine 5'-pentaphosphate, and adenosine 5'-tetraphosphate are also substrates, but not diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) or other dinucleotides, mononucleotides, nucleotide sugars, or nucleotide alcohols. Also cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate).
Mass Spectrometry Mass=21443; Method=Electrospray; Range=2-188; Evidence={ECO:0000269|PubMed:10085096};
Miscellaneous Present with 3340 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Similarity Belongs to the Nudix hydrolase family. DIPP subfamily. {ECO:0000305}.
Similarity Contains 1 nudix hydrolase domain. {ECO:0000255|PROSITE-ProRule:PRU00794}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007087 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6324737 RefSeq NP_014806 188 polyphosphatase DDP1

Identical Sequences to LMP007087 proteins

Reference Database Accession Length Protein Name
GI:6324737 GenBank EIW07593.1 188 Ddp1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6324737 GenBank EWG83259.1 188 Ddp1p [Saccharomyces cerevisiae R008]
GI:6324737 GenBank EWG88473.1 188 Ddp1p [Saccharomyces cerevisiae P301]
GI:6324737 GenBank EWG93593.1 188 Ddp1p [Saccharomyces cerevisiae R103]
GI:6324737 GenBank EWH15988.1 188 Ddp1p [Saccharomyces cerevisiae P283]
GI:6324737 gnl McCuskerlabDuke 188 Ddp1p [Saccharomyces cerevisiae YJM993]

Related Sequences to LMP007087 proteins

Reference Database Accession Length Protein Name
GI:6324737 DBBJ GAA26479.1 188 K7_Ddp1p [Saccharomyces cerevisiae Kyokai no. 7]
GI:6324737 EMBL CCC70514.1 176 hypothetical protein NCAS_0F00300 [Naumovozyma castellii CBS 4309]
GI:6324737 GenBank EDO17447.1 218 hypothetical protein Kpol_1037p44 [Vanderwaltozyma polyspora DSM 70294]
GI:6324737 GenBank EHN00180.1 188 Ddp1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:6324737 GenBank EJT43779.1 188 DDP1-like protein [Saccharomyces kudriavzevii IFO 1802]
GI:6324737 RefSeq XP_001645305.1 218 hypothetical protein Kpol_1037p44 [Vanderwaltozyma polyspora DSM 70294]