Gene/Proteome Database (LMPD)

LMPD ID
LMP007094
Gene ID
853536
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
Gene Symbol
OPI3
Synonyms
PEM2
Alternate Names
bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
Chromosome
X
EC Number
2.1.1.17

Proteins

bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
Refseq ID NP_012607
Protein GI 6322533
UniProt ID P05375
mRNA ID NM_001181731
Length 206
RefSeq Status PROVISIONAL
MKESVQEIIQQLIHSVDLQSSKFQLAIVCTMFNPIFWNIVARMEYHKHSLTKMCGGARKGCYMLAATIFSLGIVRDMVYESALREQPTCSLITGENWTKLGVALFGLGQVLVLSSMYKLGITGTYLGDYFGILMDERVTGFPFNVSNNPMYQGSTLSFLGIALYKGKPAGLVVSAVVYFMYKIALRWEEPFTAMIYANRDKAKKNM

Gene Information

Entrez Gene ID
853536
Gene Name
bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
Gene Symbol
OPI3
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 ISM:SGD C integral component of membrane
GO:0080101 IDA:SGD F phosphatidyl-N-dimethylethanolamine N-methyltransferase activity
GO:0000773 IDA:SGD F phosphatidyl-N-methylethanolamine N-methyltransferase activity
GO:0004608 IEA:UniProtKB-EC F phosphatidylethanolamine N-methyltransferase activity
GO:0006656 IDA:SGD P phosphatidylcholine biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
sce00564 Glycerophospholipid metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
5618446 Synthesis of PC

Domain Information

InterPro Annotations

Accession Description
IPR024960 Phosphatidyl-N-methylethanolamine/N-methyltransferase
IPR007318 Phospholipid methyltransferase

UniProt Annotations

Entry Information

Gene Name
bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
Protein Entry
PEM2_YEAST
UniProt ID
P05375
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=54 uM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-methylethanolamine (PMME) as substrate) {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666}; KM=59 uM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-dimethylethanolamine (PDME) as substrate) {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666}; KM=380 uM for phosphatidyl-N-methylethanolamine (PMME) {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666}; KM=180 uM for phosphatidyl-N-dimethylethanolamine (PDME) {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666}; pH dependence: Optimum pH is 8.1. {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666};
Catalytic Activity S-adenosyl-L-methionine + phosphatidyl-N- dimethylethanolamine = S-adenosyl-L-homocysteine + phosphatidylcholine. {ECO:0000255|PROSITE-ProRule:PRU00931}.
Catalytic Activity S-adenosyl-L-methionine + phosphatidyl-N- methylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N- dimethylethanolamine. {ECO:0000255|PROSITE-ProRule:PRU00931}.
Catalytic Activity S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine. {ECO:0000255|PROSITE- ProRule:PRU00931}.
Function Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC). {ECO:0000255|PROSITE-ProRule:PRU00931, ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:22001639, ECO:0000269|PubMed:2445736, ECO:0000269|PubMed:2670666, ECO:0000269|PubMed:2684666, ECO:0000269|PubMed:2850468, ECO:0000269|PubMed:6337128, ECO:0000269|PubMed:7047296}.
Induction Expression is repressed by inositol and choline. The 5' flanking region contains two copies of the CATRTGAA motif and a 5'-AAACCCACACATG-3' GRFI site, which are involved in the regulation of expression. OPI1 and SIN3 play the role of repressors for OPI3 expression whereas UME6 is an activator of OPI3 expression. {ECO:0000269|PubMed:1650774, ECO:0000269|PubMed:1954254, ECO:0000269|PubMed:6759124, ECO:0000269|PubMed:8614637}.
Miscellaneous Present with 5890 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Phospholipid metabolism; phosphatidylcholine biosynthesis. {ECO:0000255|PROSITE-ProRule:PRU00931}.
Similarity Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family. {ECO:0000255|PROSITE-ProRule:PRU00931}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007094 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6322533 RefSeq NP_012607 206 bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase

Identical Sequences to LMP007094 proteins

Reference Database Accession Length Protein Name
GI:6322533 GenBank EHN06249.1 206 Opi3p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:6322533 GenBank EIW09596.1 206 Opi3p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6322533 GenBank EWG85168.1 206 Opi3p [Saccharomyces cerevisiae R008]
GI:6322533 GenBank EWG88003.1 206 Opi3p [Saccharomyces cerevisiae P301]
GI:6322533 GenBank EWH17620.1 206 Opi3p [Saccharomyces cerevisiae P283]
GI:6322533 gnl McCuskerlabDuke 206 Opi3p [Saccharomyces cerevisiae YJM993]

Related Sequences to LMP007094 proteins

Reference Database Accession Length Protein Name
GI:6322533 EMBL CCH60862.1 263 hypothetical protein TBLA_0D03630 [Tetrapisispora blattae CBS 6284]
GI:6322533 GenBank EGA61723.1 206 Opi3p [Saccharomyces cerevisiae FostersO]
GI:6322533 GenBank EHN01642.1 228 Opi3p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:6322533 GenBank EJT41511.1 228 OPI3-like protein [Saccharomyces kudriavzevii IFO 1802]
GI:6322533 GenBank EWG95169.1 206 Opi3p [Saccharomyces cerevisiae R103]
GI:6322533 RefSeq XP_004180381.1 263 hypothetical protein TBLA_0D03630 [Tetrapisispora blattae CBS 6284]