LIPID MAPS

LMPD ID

LMP007101

Gene ID

853608

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Pmt4p

Gene Symbol

PMT4

Synonyms

-

Chromosome

X

EC Number

2.4.1.109 ;

Pmt4p
Refseq ID	NP_012677
Protein GI	6322603
UniProt ID	P46971
mRNA ID	NM_001181801
Length	762
MSVPKKRNHGKLPPSTKDVDDPSLKYTKAAPKCEQVAEHWLLQPLPEPESRYSFWVTIVTLLAFAARFYKIWYPKEVVFDEVHFGKFASYYLERSYFFDVHPPFAKMMIAFIGWLCGYDGSFKFDEIGYSYETHPAPYIAYRSFNAILGTLTVPIMFNTLKELNFRAITCAFASLLVAIDTAHVTETRLILLDAILIISIAATMYCYVRFYKCQLRQPFTWSWYIWLHATGLSLSFVISTKYVGVMTYSAIGFAAVVNLWQLLDIKAGLSLRQFMRHFSKRLNGLVLIPFVIYLFWFWVHFTVLNTSGPGDAFMSAEFQETLKDSPLSVDSKTVNYFDIITIKHQDTDAFLHSHLARYPQRYEDGRISSAGQQVTGYTHPDFNNQWEVLPPHGSDVGKGQAVLLNQHIRLRHVATDTYLLAHDVASPFYPTNEEITTVTLEEGDGELYPETLFAFQPLKKSDEGHVLKSKTVSFRLFHVDTSVALWTHNDELLPDWGFQQQEINGNKKVIDPSNNWVVDEIVNLDEVRKVYIPKVVKPLPFLKKWIETQKSMFEHNNKLSSEHPFASEPYSWPGSLSGVSFWTNGDEKKQIYFIGNIIGWWFQVISLAVFVGIIVADLITRHRGYYALNKMTREKLYGPLMFFFVSWCCHYFPFFLMARQKFLHHYLPAHLIACLFSGALWEVIFSDCKSLDLEKDEDISGASYERNPKVYVKPYTVFLVCVSCAVAWFFVYFSPLVYGDVSLSPSEVVSREWFDIELNFSK

Entrez Gene ID

853608

Gene Name

Pmt4p

Gene Symbol

PMT4

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0097586	IPI:SGD	C	dolichyl-phosphate-mannose-protein mannosyltransferase Pmt4p homodimer complex
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0004169	IDA:SGD	F	dolichyl-phosphate-mannose-protein mannosyltransferase activity
GO:0042802	IPI:IntAct	F	identical protein binding
GO:0006493	IMP:SGD	P	protein O-linked glycosylation
GO:0035269	IMP:SGD	P	protein O-linked mannosylation
GO:1900101	IGI:SGD	P	regulation of endoplasmic reticulum unfolded protein response

KEGG Pathway Links

KEGG Pathway ID	Description
ko00514	Other types of O-glycan biosynthesis
sce00514	Other types of O-glycan biosynthesis

InterPro Annotations

Accession	Description
IPR003342	Glycosyl transferase, family 39
IPR027005	Glycosyltransferase 39 like
IPR016093	MIR_motif

Entry Information

Gene Name

Pmt4p

Protein Entry

PMT4_YEAST

UniProt ID

P46971

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	Dolichyl D-mannosyl phosphate + protein = dolichyl phosphate + O-D-mannosylprotein
Catalytic Activity	Dolichyl D-mannosyl phosphate + protein = dolichyl phosphate + O-D-mannosylprotein. {ECO:0000269\|PubMed:17470820}.
Disruption Phenotype	Leads to diminished MID2 O-mannosylation
Disruption Phenotype	Leads to diminished MID2 O-mannosylation. {ECO:0000269\|PubMed:14673142}.
Function	Protein O-mannosyltransferase involved in O- glycosylation which is essential for cell wall rigidity. Forms a homodimeric complex to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. Specifically acts on secretory proteins with an ER-luminally oriented Ser/Thr-rich region flanked by a membrane anchor such as FUS1, AXL2, GAS1, KEX2, MID2, WSC1, WSC2, OPY2, PRM5, RAX2, or YNL176. {ECO:0000269\|PubMed:10366591, ECO:0000269\|PubMed:14673142, ECO:0000269\|PubMed:17470820, ECO:0000269\|PubMed:9184828}.
Interaction	Self; NbExp=2; IntAct=EBI-13585, EBI-13585;
Miscellaneous	Present with 1270 molecules/cell in log phase SD medium
Miscellaneous	Present with 1270 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Protein modification; protein glycosylation
Pathway	Protein modification; protein glycosylation. {ECO:0000305}.
Similarity	Belongs to the glycosyltransferase 39 family
Similarity	Belongs to the glycosyltransferase 39 family. {ECO:0000305}.
Similarity	Contains 3 MIR domains. {ECO:0000255\|PROSITE- ProRule:PRU00131}.
Subcellular Location	Endoplasmic reticulum membrane ; Multi-pass membrane protein .
Subcellular Location	Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P33775}; Multi-pass membrane protein {ECO:0000255}.
Subunit	Forms a functional homodimer and may form a heterodimer with PMT6. Interacts with RCR1. {ECO:0000269\|PubMed:12551906, ECO:0000269\|PubMed:17213653, ECO:0000303\|PubMed:9184828}.