Gene/Proteome Database (LMPD)

LMPD ID
LMP007109
Gene ID
854276
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53
Gene Symbol
INP53
Synonyms
SJL3; SOP2
Alternate Names
phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53
Chromosome
XV
EC Number
3.1.3.-

Proteins

phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53
Refseq ID NP_014752
Protein GI 398365141
UniProt ID Q12271
mRNA ID NM_001183528
Length 1107
RefSeq Status PROVISIONAL
MIIFVSEEPERRLAIVSNLYALVLKPVGKKPSDKPLCAIELLQKNDLKKYGFKRLTSHEIFGVIGLIEVNGLLFVGAITGKSKVAQPCPGETVNKIFAVDFFCLNDNSWDFIEIDSSGYPVLPETASTEYQDALPKHPCYELKKLLSNGSFYYSSDFDLTSTLQHRGYGQHSLSTDTYEEEYMWNSFLMQEMITYRDHLDTNLKQILDDEGFLTTVIRGFAETFVSYVKKLKVALTIISKQSWKRAGTRFNARGVDDEANVANFVETEFIMYSSQYCYAFTQIRGSIPVFWEQGTSLINPRVQITRSFEATQPVFDKHIMKSVEKYGPVHVVNLLSTKSSEIELSKRYKEHLTHSKKLNFNKDIFLTEFDFHKETSQEGFSGVRKLIPLILDSLLSSGYYSYDVREKKNISEQHGIFRTNCLDCLDRTNLAQQIISLAAFRTFLEDFRLISSNSFIDDDDFVSKHNTLWADHGDQISQIYTGTNALKSSFSRKGKMSLAGALSDATKSVSRIYINNFMDKEKQQNIDTLLGRLPYQKAVQLYDPVNEYVSTKLQSMSDKFTSTSNINLLIGSFNVNGATKKVDLSKWLFPIGEKFKPDIVVLGLQEVIELSAGSILNADYSKSSFWENLVGDCLNQYDDKYLLLRVEQMTSLLILFFVKADKAKYVKQVEGATKKTGFRGMAGNKGAVSIRFEYGATSFCFVNSHLAAGATNVEERRSDYESIVRGITFTRTKMIPHHDSIFWLGDMNYRINLPNEDVRRELLNQEEGYIDKLLHFDQLTLGINSGSVFEGFKEPTLKFRPTYKYDPGTGTYDSSEKERTPSWTDRIIYKGENLLPLSYSDAPIMISDHRPVYAAYRAKITFVDDKERLSLKKRLFTEYKQEHPEEPGSLISDLLSLDLDNKSTDGFKSSSESSLLDIDPIMAQPTASSVASSSPVSSASASLQPVRTQNSSQSRTPIKKPVLRPPPPPAHKSVSAPAPSTSKEKSPTPQTSTASLSSVTKNIQENKPLAQNRRIPPPGFSQNILTPKSTSNLASPMSSKVDLYNSASESTRSAQDARQQTPTAFAASRDVNGQPEALLGDENPIEPEEKAKLNHMTLDSWQPLTPK

Gene Information

Entrez Gene ID
854276
Gene Name
phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53
Gene Symbol
INP53
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0030479 IDA:SGD C actin cortical patch
GO:0005737 IDA:SGD C cytoplasm
GO:0016020 IDA:SGD C membrane
GO:0043813 IDA:SGD F phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity
GO:0004438 IDA:SGD F phosphatidylinositol-3-phosphatase activity
GO:0004439 IDA:SGD F phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity
GO:0043812 IDA:SGD F phosphatidylinositol-4-phosphate phosphatase activity
GO:0006897 IEA:UniProtKB-KW P endocytosis
GO:0046856 IDA:SGD P phosphatidylinositol dephosphorylation
GO:0036092 IDA:GOC P phosphatidylinositol-3-phosphate biosynthetic process
GO:0015031 IEA:UniProtKB-KW P protein transport

KEGG Pathway Links

KEGG Pathway ID Description
sce00562 Inositol phosphate metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY3O-242 phosphatidylinositol phosphate biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5618625 Synthesis of IP2, IP, and Ins in the cytosol
5618610 Synthesis of PIPs at the plasma membrane

Domain Information

InterPro Annotations

Accession Description
IPR005135 Endonuclease/exonuclease/phosphatase
IPR000300 Inositol polyphosphate-related phosphatase
IPR002013 SAC domain

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53
Protein Entry
INP53_YEAST
UniProt ID
Q12271
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Domain The 5-phosphatase domain (residues 568 to 856) selectively removes the phosphate group at the 5' position of inositol of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).
Domain The C-terminal proline-rich domain is required for the function and associates with clathrin heavy chain CHC1.
Domain The SAC1 domain is capable of hydrolyzing phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4-phosphate (PtdIns(4)P), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
Function Dephosphorylates a number of phosphatidylinositols (PIs) like phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), but also phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4-phosphate (PtdIns(4)P), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Controls the cellular levels and subcellular distribution of phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5- bisphosphate. Plays an essential role in a TGN (trans Golgi network)-to-early endosome pathway. Involved in clathrin-mediated protein sorting at the TGN. {ECO:0000269|PubMed:10029994, ECO:0000269|PubMed:10224048, ECO:0000269|PubMed:10625610, ECO:0000269|PubMed:10628971, ECO:0000269|PubMed:11094088, ECO:0000269|PubMed:11598201, ECO:0000269|PubMed:12686590, ECO:0000269|PubMed:15169871, ECO:0000269|PubMed:15691741, ECO:0000269|PubMed:9265642, ECO:0000269|PubMed:9560389, ECO:0000269|PubMed:9788876}.
Miscellaneous Present with 1520 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Similarity Belongs to the synaptojanin family. {ECO:0000305}.
Similarity Contains 1 SAC domain. {ECO:0000255|PROSITE- ProRule:PRU00183}.
Similarity In the central section; belongs to the inositol 1,4,5- trisphosphate 5-phosphatase family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:11094088, ECO:0000269|PubMed:14562095}. Note=Cytoplasmic punctate structures. Hyperosmotic stress causes translocation to actin patches.
Subunit Interacts with BSP1 and CHC1. {ECO:0000269|PubMed:12606027, ECO:0000269|PubMed:12686590}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007109 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
398365141 RefSeq NP_014752 1107 phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53

Identical Sequences to LMP007109 proteins

Reference Database Accession Length Protein Name
GI:398365141 EMBL CAA99307.1 1107 PIE2 [Saccharomyces cerevisiae]
GI:398365141 EMBL CAR81399.1 1107 unnamed protein product [Saccharomyces cerevisiae]
GI:398365141 EMBL CBX84861.1 1107 unnamed protein product [Saccharomyces cerevisiae]
GI:398365141 GenBank EIW07540.1 1107 Inp53p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:398365141 SwissProt Q12271.1 1107 RecName: Full=Polyphosphatidylinositol phosphatase INP53; AltName: Full=Suppressor of PMA1 protein 2; AltName: Full=Synaptojanin-like protein 3; Includes: RecName: Full=SAC1-like phosphoinositide phosphatase; Includes: RecName: Full=Phosphatidylinositol 4,5-bisphosphate 5-phosphatase [Saccharomyces cerevisiae S288c]
GI:398365141 Third Party Genbank DAA10884.1 1107 TPA: phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53 [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007109 proteins

Reference Database Accession Length Protein Name
GI:398365141 DBBJ GAA26428.1 1107 K7_Inp53p [Saccharomyces cerevisiae Kyokai no. 7]
GI:398365141 EMBL CAY86396.1 1107 Inp53p [Saccharomyces cerevisiae EC1118]
GI:398365141 GenBank EEU07953.1 1107 Inp53p [Saccharomyces cerevisiae JAY291]
GI:398365141 GenBank EHN04590.1 1107 Inp53p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:398365141 GenBank EWG83211.1 1107 Inp53p [Saccharomyces cerevisiae R008]
GI:398365141 GenBank EWH15944.1 1107 Inp53p [Saccharomyces cerevisiae P283]