LIPID MAPS

LMPD ID

LMP007122

Gene ID

850941

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

phosphatidylinositol 3-kinase VPS34

Gene Symbol

VPS34

Synonyms

END12; PEP15; STT8; VPL7; VPS7; VPT29

Chromosome

XII

EC Number

2.7.1.137

phosphatidylinositol 3-kinase VPS34
Refseq ID	NP_013341
Protein GI	6323269
UniProt ID	P22543
mRNA ID	NM_001182127
Length	875
MSLNNITFCVSQDLDVPLKVKIKSLEGHKPLLKPSQKILNPELMLIGSNVFPSSDLIVSLQVFDKERNRNLTLPIYTPYIPFRNSRTWDYWLTLPIRIKQLTFSSHLRIILWEYNGSKQIPFFNLETSIFNLKDCTLKRGFESLKFRYDVIDHCEVVTDNKDQENLNKYFQGEFTRLPWLDEITISKLRKQRENRTWPQGTFVLNLEFPMLELPVVFIEREIMNTQMNIPTLKNNPGLSTDLREPNRNDPQIKISLGDKYHSTLKFYDPDQPNNDPIEEKYRRLERASKNANLDKQVKPDIKKRDYLNKIINYPPGTKLTAHEKGSIWKYRYYLMNNKKALTKLLQSTNLREESERVEVLELMDSWAEIDIDDALELLGSTFKNLSVRSYAVNRLKKASDKELELYLLQLVEAVCFENLSTFSDKSNSEFTIVDAVSSQKLSGDSMLLSTSHANQKLLKSISSESETSGTESLPIVISPLAEFLIRRALVNPRLGSFFYWYLKSESEDKPYLDQILSSFWSRLDKKSRNILNDQVRLINVLRECCETIKRLKDTTAKKMELLVHLLETKVRPLVKVRPIALPLDPDVLICDVCPETSKVFKSSLSPLKITFKTTLNQPYHLMFKVGDDLRQDQLVVQIISLMNELLKNENVDLKLTPYKILATGPQEGAIEFIPNDTLASILSKYHGILGYLKLHYPDENATLGVQGWVLDNFVKSCAGYCVITYILGVGDRHLDNLLVTPDGHFFHADFGYILGQDPKPFPPLMKLPPQIIEAFGGAESSNYDKFRSYCFVAYSILRRNAGLILNLFELMKTSNIPDIRIDPNGAILRVRERFNLNMSEEDATVHFQNLINDSVNALLPIVIDHLHNLAQYWRT

Entrez Gene ID

850941

Gene Name

phosphatidylinositol 3-kinase VPS34

Gene Symbol

VPS34

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005768	IDA:SGD	C	endosome
GO:0000329	IDA:SGD	C	fungal-type vacuole membrane
GO:0005794	IEA:UniProtKB-KW	C	Golgi apparatus
GO:0071561	IDA:SGD	C	nucleus-vacuole junction
GO:0005777	IDA:SGD	C	peroxisome
GO:0034271	IDA:SGD	C	phosphatidylinositol 3-kinase complex, class III, type I
GO:0034272	IDA:SGD	C	phosphatidylinositol 3-kinase complex, class III, type II
GO:0000407	IDA:SGD	C	pre-autophagosomal structure
GO:0016303	IDA:SGD	F	1-phosphatidylinositol-3-kinase activity
GO:0005524	IEA:UniProtKB-KW	F	ATP binding
GO:0004672	IDA:SGD	F	protein kinase activity
GO:0030242	IMP:SGD	P	peroxisome degradation
GO:0036092	IDA:GOC	P	phosphatidylinositol-3-phosphate biosynthetic process
GO:0048015	IEA:InterPro	P	phosphatidylinositol-mediated signaling
GO:0046854	IDA:SGD	P	phosphatidylinositol phosphorylation
GO:0032968	IMP:SGD	P	positive regulation of transcription elongation from RNA polymerase II promoter
GO:0006468	IDA:SGD	P	protein phosphorylation
GO:0015031	IEA:UniProtKB-KW	P	protein transport

KEGG Pathway Links

KEGG Pathway ID	Description
ko00562	Inositol phosphate metabolism
sce00562	Inositol phosphate metabolism
sce01100	Metabolic pathways
ko04145	Phagosome
sce04145	Phagosome
ko04070	Phosphatidylinositol signaling system
sce04070	Phosphatidylinositol signaling system
ko04140	Regulation of autophagy
sce04140	Regulation of autophagy

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY3O-242	phosphatidylinositol phosphate biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618126	Phospholipid metabolism
5618611	PI Metabolism
5618615	Synthesis of PIPs at the early endosome membrane
5618614	Synthesis of PIPs at the Golgi membrane
5618612	Synthesis of PIPs at the late endosome membrane

InterPro Annotations

Accession	Description
IPR016024	Armadillo-type fold
IPR000008	C2 domain
IPR000403	Phosphatidylinositol 3-/4-kinase, catalytic domain
IPR018936	Phosphatidylinositol 3/4-kinase, conserved site
IPR002420	Phosphatidylinositol 3-kinase, C2 domain
IPR008290	Phosphatidylinositol 3-kinase, Vps34 type
IPR015433	Phosphatidylinositol Kinase
IPR001263	Phosphoinositide 3-kinase, accessory (PIK) domain
IPR011009	Protein kinase-like domain

Entry Information

Gene Name

phosphatidylinositol 3-kinase VPS34

Protein Entry

VPS34_YEAST

UniProt ID

P22543

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Enzyme Regulation	Phosphatidylinositol 3-kinase activity is directly dependent on VPS15 protein kinase activity.
Function	Phosphatidylinositol 3-kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy- specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3- kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Its activation by VPS15 may lead to the phosphorylation of phosphatidylinositol in the sorting compartment membrane. Finally, it might also be involved in ethanol tolerance and cell wall integrity. {ECO:0000269\|PubMed:11157979, ECO:0000269\|PubMed:11523784, ECO:0000269\|PubMed:12244127, ECO:0000269\|PubMed:2247081, ECO:0000269\|PubMed:2555343, ECO:0000269\|PubMed:3062374, ECO:0000269\|PubMed:7929582, ECO:0000269\|PubMed:7989323, ECO:0000269\|PubMed:8385367, ECO:0000269\|PubMed:8387919}.
Interaction	P08539:GPA1; NbExp=3; IntAct=EBI-20405, EBI-7376;
Miscellaneous	Present with 1080 molecules/cell in log phase SD medium
Miscellaneous	Present with 1080 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Ptm	Autophosphorylated. Might also be phosphorylated by VPS15.
Similarity	Belongs to the PI3/PI4-kinase family
Similarity	Belongs to the PI3/PI4-kinase family. {ECO:0000255\|PROSITE-ProRule:PRU00880}.
Similarity	Contains 1 C2 PI3K-type domain. {ECO:0000255\|PROSITE- ProRule:PRU00880}.
Similarity	Contains 1 PI3K/PI4K domain. {ECO:0000255\|PROSITE- ProRule:PRU00269}.
Similarity	Contains 1 PIK helical domain. {ECO:0000255\|PROSITE- ProRule:PRU00878}.
Subcellular Location	Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.
Subunit	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14, AND ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38. Interacts directly with ATG38. {ECO:0000269\|PubMed:11157979, ECO:0000269\|PubMed:24165940, ECO:0000269\|PubMed:8387919}.