Gene/Proteome Database (LMPD)

LMPD ID
LMP007129
Gene ID
850527
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
dihydrolipoyl dehydrogenase
Gene Symbol
LPD1
Synonyms
HPD1
Alternate Names
dihydrolipoyl dehydrogenase
Chromosome
VI
EC Number
1.8.1.4

Proteins

dihydrolipoyl dehydrogenase
Refseq ID NP_116635
Protein GI 14318501
UniProt ID P09624
mRNA ID NM_001179948
Length 499
RefSeq Status PROVISIONAL
MLRIRSLLNNKRAFSSTVRTLTINKSHDVVIIGGGPAGYVAAIKAAQLGFNTACVEKRGKLGGTCLNVGCIPSKALLNNSHLFHQMHTEAQKRGIDVNGDIKINVANFQKAKDDAVKQLTGGIELLFKKNKVTYYKGNGSFEDETKIRVTPVDGLEGTVKEDHILDVKNIIVATGSEVTPFPGIEIDEEKIVSSTGALSLKEIPKRLTIIGGGIIGLEMGSVYSRLGSKVTVVEFQPQIGASMDGEVAKATQKFLKKQGLDFKLSTKVISAKRNDDKNVVEIVVEDTKTNKQENLEAEVLLVAVGRRPYIAGLGAEKIGLEVDKRGRLVIDDQFNSKFPHIKVVGDVTFGPMLAHKAEEEGIAAVEMLKTGHGHVNYNNIPSVMYSHPEVAWVGKTEEQLKEAGIDYKIGKFPFAANSRAKTNQDTEGFVKILIDSKTERILGAHIIGPNAGEMIAEAGLALEYGASAEDVARVCHAHPTLSEAFKEANMAAYDKAIHC

Gene Information

Entrez Gene ID
850527
Gene Name
dihydrolipoyl dehydrogenase
Gene Symbol
LPD1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005960 IMP:SGD C glycine cleavage complex
GO:0042645 IDA:SGD C mitochondrial nucleoid
GO:0009353 IDA:SGD C mitochondrial oxoglutarate dehydrogenase complex
GO:0005967 IDA:SGD C mitochondrial pyruvate dehydrogenase complex
GO:0004148 IDA:SGD F dihydrolipoyl dehydrogenase activity
GO:0050660 IEA:InterPro F flavin adenine dinucleotide binding
GO:0004375 IMP:SGD F glycine dehydrogenase (decarboxylating) activity
GO:0004591 IMP:SGD F oxoglutarate dehydrogenase (succinyl-transferring) activity
GO:0004738 IMP:SGD F pyruvate dehydrogenase activity
GO:0006103 IMP:SGD P 2-oxoglutarate metabolic process
GO:0006564 IMP:SGD P L-serine biosynthetic process
GO:0045454 IEA:InterPro P cell redox homeostasis
GO:0006546 IMP:SGD P glycine catabolic process
GO:0042743 IMP:SGD P hydrogen peroxide metabolic process
GO:0006550 IMP:SGD P isoleucine catabolic process
GO:0006552 IMP:SGD P leucine catabolic process
GO:0006090 IMP:SGD P pyruvate metabolic process
GO:0006574 IMP:SGD P valine catabolic process

KEGG Pathway Links

KEGG Pathway ID Description
sce00020 Citrate cycle (TCA cycle)
sce00260 Glycine, serine and threonine metabolism
sce00010 Glycolysis / Gluconeogenesis

Domain Information

InterPro Annotations

Accession Description
IPR006258 Dihydrolipoamide dehydrogenase
IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase
IPR016156 FAD/NAD-linked reductase, dimerisation domain
IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain
IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain
IPR012999 Pyridine nucleotide-disulphide oxidoreductase, class I, active site
IPR004099 Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain

UniProt Annotations

Entry Information

Gene Name
dihydrolipoyl dehydrogenase
Protein Entry
DLDH_YEAST
UniProt ID
P09624
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH.
Cofactor Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250}; Note=Binds 1 FAD per subunit. {ECO:0000250};
Function Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes. This includes the pyruvate dehydrogenase complex, which catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). Acts also as component of the glycine cleavage system (glycine decarboxylase complex), which catalyzes the degradation of glycine.
Miscellaneous Present with 24600 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Miscellaneous The active site is a redox-active disulfide bond.
Similarity Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. {ECO:0000305}.
Subcellular Location Mitochondrion matrix.
Subunit LPD1 is a homodimer. Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds. LPD1 is a component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H. {ECO:0000269|PubMed:7498764, ECO:0000269|PubMed:9538259, ECO:0000269|Ref.9}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007129 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
14318501 RefSeq NP_116635 499 dihydrolipoyl dehydrogenase

Identical Sequences to LMP007129 proteins

Reference Database Accession Length Protein Name
GI:14318501 GenBank AEU53537.1 499 Sequence 168 from patent US 8062891
GI:14318501 GenBank AGO72490.1 499 Sequence 168 from patent US 8470972
GI:14318501 GenBank AGP21944.1 499 Sequence 108 from patent US 8465964
GI:14318501 GenBank AGU32481.1 499 Sequence 168 from patent US 8507277
GI:14318501 GenBank AGX48385.1 499 Sequence 168 from patent US 8541550
GI:14318501 GenBank AIB02139.1 499 Sequence 89 from patent US 8669094

Related Sequences to LMP007129 proteins

Reference Database Accession Length Protein Name
GI:14318501 GenBank EDV09821.1 499 dihydrolipoamide dehydrogenase precursor [Saccharomyces cerevisiae RM11-1a]
GI:14318501 GenBank EIW10746.1 499 Lpd1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:14318501 GenBank EWG86345.1 499 Lpd1p [Saccharomyces cerevisiae R008]
GI:14318501 GenBank EWG91251.1 499 Lpd1p [Saccharomyces cerevisiae P301]
GI:14318501 GenBank EWG96247.1 499 Lpd1p [Saccharomyces cerevisiae R103]
GI:14318501 GenBank EWH18687.1 499 Lpd1p [Saccharomyces cerevisiae P283]