Gene/Proteome Database (LMPD)

LMPD ID
LMP007212
Gene ID
855342
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
serine C-palmitoyltransferase LCB1
Gene Symbol
LCB1
Synonyms
END8; TSC2
Alternate Names
serine C-palmitoyltransferase LCB1
Chromosome
XIII
EC Number
2.3.1.50

Proteins

serine C-palmitoyltransferase LCB1
Refseq ID NP_014025
Protein GI 6323954
UniProt ID P25045
mRNA ID NM_001182805
Length 558
RefSeq Status PROVISIONAL
MAHIPEVLPKSIPIPAFIVTTSSYLWYYFNLVLTQIPGGQFIVSYIKKSHHDDPYRTTVEIGLILYGIIYYLSKPQQKKSLQAQKPNLSPQEIDALIEDWEPEPLVDPSATDEQSWRVAKTPVTMEMPIQNHITITRNNLQEKYTNVFNLASNNFLQLSATEPVKEVVKTTIKNYGVGACGPAGFYGNQDVHYTLEYDLAQFFGTQGSVLYGQDFCAAPSVLPAFTKRGDVIVADDQVSLPVQNALQLSRSTVYYFNHNDMNSLECLLNELTEQEKLEKLPAIPRKFIVTEGIFHNSGDLAPLPELTKLKNKYKFRLFVDETFSIGVLGATGRGLSEHFNMDRATAIDITVGSMATALGSTGGFVLGDSVMCLHQRIGSNAYCFSACLPAYTVTSVSKVLKLMDSNNDAVQTLQKLSKSLHDSFASDDSLRSYVIVTSSPVSAVLHLQLTPAYRSRKFGYTCEQLFETMSALQKKSQTNKFIEPYEEEEKFLQSIVDHALINYNVLITRNTIVLKQETLPIVPSLKICCNAAMSPEELKNACESVKQSILACCQESNK

Gene Information

Entrez Gene ID
855342
Gene Name
serine C-palmitoyltransferase LCB1
Gene Symbol
LCB1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0035339 IDA:UniProtKB C SPOTS complex
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0017059 IMP:SGD C serine C-palmitoyltransferase complex
GO:0030170 IEA:InterPro F pyridoxal phosphate binding
GO:0004758 IMP:SGD F serine C-palmitoyltransferase activity
GO:0030148 IMP:SGD P sphingolipid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
sce00600 Sphingolipid metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID Description
SPHINGOLIPID-SYN-PWY sphingolipid biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5618508 Sphingolipid de novo biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR004839 Aminotransferase, class I/classII
IPR015424 Pyridoxal phosphate-dependent transferase
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1

UniProt Annotations

Entry Information

Gene Name
serine C-palmitoyltransferase LCB1
Protein Entry
LCB1_YEAST
UniProt ID
P25045
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D- sphinganine + CO(2). {ECO:0000269|PubMed:10713067, ECO:0000269|PubMed:11781309, ECO:0000269|PubMed:15485854, ECO:0000269|PubMed:8058731}.
Cofactor Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
Domain The first transmembrane domain is not required for stability, membrane association, interaction with LCB2, or enzymatic activity. The second and third transmembrane domains are required for stability and interaction with LCB2.
Function Component of serine palmitoyltransferase (SPT), which catalyzes the committed step in the synthesis of sphingolipids, the condensation of serine with palmitoyl CoA to form the long chain base 3-ketosphinganine. {ECO:0000269|PubMed:11781309, ECO:0000269|PubMed:8058731}.
Interaction P40970:LCB2; NbExp=9; IntAct=EBI-10059, EBI-10067;
Miscellaneous Present with 22400 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Lipid metabolism; sphingolipid metabolism.
Similarity Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. {ECO:0000305}.
Subcellular Location Cytoplasm. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Subunit LCB1 and LCB2 encode essential subunits of the enzyme and form a heterodimer. Component of the SPOTS complex, at least composed of LCB1/2 (LCB1 and/or LCB2), ORM1/2 (ORM1 and/or ORM2), SAC1 and TSC3. Interacts with LCB2 and TSC3. {ECO:0000269|PubMed:10713067, ECO:0000269|PubMed:11781309, ECO:0000269|PubMed:15485854, ECO:0000269|PubMed:20182505, ECO:0000269|PubMed:8058731}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007212 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6323954 RefSeq NP_014025 558 serine C-palmitoyltransferase LCB1

Identical Sequences to LMP007212 proteins

Reference Database Accession Length Protein Name
GI:6323954 GenBank EGA60868.1 558 Lcb1p [Saccharomyces cerevisiae FostersO]
GI:6323954 GenBank EGA73610.1 558 Lcb1p [Saccharomyces cerevisiae AWRI796]
GI:6323954 GenBank EGA85519.1 558 Lcb1p [Saccharomyces cerevisiae VL3]
GI:6323954 GenBank EWG84205.1 558 Lcb1p [Saccharomyces cerevisiae R008]
GI:6323954 GenBank EWG93935.1 558 Lcb1p [Saccharomyces cerevisiae R103]
GI:6323954 GenBank EWH16877.1 558 Lcb1p [Saccharomyces cerevisiae P283]

Related Sequences to LMP007212 proteins

Reference Database Accession Length Protein Name
GI:6323954 EMBL CAY82131.1 558 Lcb1p [Saccharomyces cerevisiae EC1118]
GI:6323954 GenBank AAA34739.1 558 serine palmitoyltransferase [Saccharomyces cerevisiae]
GI:6323954 GenBank EGA81263.1 558 Lcb1p [Saccharomyces cerevisiae Lalvin QA23]
GI:6323954 GenBank EHN05516.1 558 Lcb1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:6323954 GenBank EIW08571.1 558 Lcb1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6323954 gnl McCuskerlabDuke 558 Lcb1p [Saccharomyces cerevisiae YJM993]