Gene/Proteome Database (LMPD)

LMPD ID
LMP007239
Gene ID
854758
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
glycerol-1-phosphatase RHR2
Gene Symbol
GPP1
Synonyms
RHR2
Alternate Names
glycerol-1-phosphatase RHR2
Chromosome
IX
EC Number
3.1.3.21

Proteins

glycerol-1-phosphatase RHR2
Refseq ID NP_012211
Protein GI 86558907
UniProt ID P41277
mRNA ID NM_001179403
Length 250
RefSeq Status PROVISIONAL
MPLTTKPLSLKINAALFDVDGTIIISQPAIAAFWRDFGKDKPYFDAEHVIHISHGWRTYDAIAKFAPDFADEEYVNKLEGEIPEKYGEHSIEVPGAVKLCNALNALPKEKWAVATSGTRDMAKKWFDILKIKRPEYFITANDVKQGKPHPEPYLKGRNGLGFPINEQDPSKSKVVVFEDAPAGIAAGKAAGCKIVGIATTFDLDFLKEKGCDIIVKNHESIRVGEYNAETDEVELIFDDYLYAKDDLLKW

Gene Information

Entrez Gene ID
854758
Gene Name
glycerol-1-phosphatase RHR2
Gene Symbol
GPP1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0000121 IDA:SGD F glycerol-1-phosphatase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0016311 IDA:GOC P dephosphorylation
GO:0006114 IMP:SGD P glycerol biosynthetic process
GO:0006970 TAS:SGD P response to osmotic stress

KEGG Pathway Links

KEGG Pathway ID Description
sce00561 Glycerolipid metabolism
sce01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY3O-48 glycerol biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR006439 HAD hydrolase, subfamily IA
IPR023214 HAD-like domain
IPR023198 Phosphoglycolate phosphatase, domain 2

UniProt Annotations

Entry Information

Gene Name
glycerol-1-phosphatase RHR2
Protein Entry
GPP1_YEAST
UniProt ID
P41277
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=3.1 mM for (DL)-glycerol 3-phosphate {ECO:0000269|PubMed:8662716}; Vmax=49 umol/min/mg enzyme {ECO:0000269|PubMed:8662716}; pH dependence: Optimum pH is 6.5 {ECO:0000269|PubMed:8662716};
Catalytic Activity Glycerol 1-phosphate + H(2)O = glycerol + phosphate. {ECO:0000269|PubMed:8662716}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305|PubMed:8662716};
Disruption Phenotype Leads to osmosensitivity. {ECO:0000269|PubMed:10931288}.
Function Major isoform of glycerol-1-phosphate phosphohydrolase involved in glycerol biosynthesis. Plays a role in osmoadaptation and required for adaptation to anaerobic conditions. {ECO:0000269|PubMed:11058591, ECO:0000269|PubMed:15113568, ECO:0000269|PubMed:16358322, ECO:0000269|PubMed:18438874, ECO:0000269|PubMed:8662716}.
Induction In response to both anaerobic and osmotic stress. Expression seems to be under the control of YIG1. {ECO:0000269|PubMed:11058591, ECO:0000269|PubMed:11113971, ECO:0000269|PubMed:15066826, ECO:0000269|PubMed:16358322, ECO:0000269|PubMed:17425669, ECO:0000269|PubMed:20803479}.
Interaction Q08956:YIG1; NbExp=3; IntAct=EBI-7829, EBI-30385;
Miscellaneous Present with 193000 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Sequence Caution Sequence=CAA86169.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Similarity Belongs to the HAD-like hydrolase superfamily. DOG/GPP family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}.
Subunit Monomer. {ECO:0000269|PubMed:8662716}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007239 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
86558907 RefSeq NP_012211 250 glycerol-1-phosphatase RHR2

Identical Sequences to LMP007239 proteins

Reference Database Accession Length Protein Name
GI:86558907 GenBank EIW09761.1 250 Rhr2p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:86558907 GenBank EWG85345.1 250 Rhr2p [Saccharomyces cerevisiae R008]
GI:86558907 GenBank EWG90509.1 250 Rhr2p [Saccharomyces cerevisiae P301]
GI:86558907 GenBank EWG95482.1 250 Rhr2p [Saccharomyces cerevisiae R103]
GI:86558907 GenBank EWH17915.1 250 Rhr2p [Saccharomyces cerevisiae P283]
GI:86558907 gnl McCuskerlabDuke 250 Rhr2p [Saccharomyces cerevisiae YJM993]

Related Sequences to LMP007239 proteins

Reference Database Accession Length Protein Name
GI:86558907 EMBL CAR81036.1 271 unnamed protein product [Saccharomyces cerevisiae]
GI:86558907 EMBL CAY34811.1 271 unnamed protein product [Saccharomyces cerevisiae]
GI:86558907 GenBank ACH16317.1 271 Sequence 14 from patent US 7402415
GI:86558907 GenBank EDZ71495.1 271 YIL053Wp-like protein [Saccharomyces cerevisiae AWRI1631]
GI:86558907 GenBank ACP88248.1 271 Sequence 107 from patent US 7504250
GI:86558907 GenBank AFD46027.1 271 Sequence 6 from patent US 8129170