Gene/Proteome Database (LMPD)

LMPD ID
LMP007241
Gene ID
852388
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
Tim12p
Gene Symbol
TIM12
Synonyms
MRS5
Alternate Names
Tim12p
Chromosome
II

Proteins

Tim12p
Refseq ID NP_009649
Protein GI 6319567
UniProt ID P32830
mRNA ID NM_001178439
Length 109
RefSeq Status PROVISIONAL
MSFFLNSLRGNQEVSQEKLDVAGVQFDAMCSTFNNILSTCLEKCIPHEGFGEPDLTKGEQCCIDRCVAKMHYSNRLIGGFVQTRGFGPENQLRHYSRFVAKEIADDSKK

Gene Information

Entrez Gene ID
852388
Gene Name
Tim12p
Gene Symbol
TIM12
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005743 TAS:Reactome C mitochondrial inner membrane
GO:0042721 IDA:SGD C mitochondrial inner membrane protein insertion complex
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0005543 IDA:SGD F phospholipid binding
GO:0045039 IDA:SGD P protein import into mitochondrial inner membrane

Domain Information

InterPro Annotations

Accession Description
IPR027247 Mitochondrial import inner membrane translocase subunit Tim10/Tim12
IPR027102 Mitochondrial import inner membrane translocase subunit Tim12
IPR004217 Tim10/DDP family zinc finger

UniProt Annotations

Entry Information

Gene Name
Tim12p
Protein Entry
TIM12_YEAST
UniProt ID
P32830
Species
Yeast (S288c)

Comments

Comment Type Description
Domain The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM12 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (By similarity). {ECO:0000250}.
Function Essential component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. In the TIM22 complex, it acts as a docking point for the soluble TIM9-TIM10 heterohexamer that guides the target proteins in transit through the aqueous mitochondrial intermembrane space. {ECO:0000269|PubMed:10369662, ECO:0000269|PubMed:10995434, ECO:0000269|PubMed:8663351, ECO:0000269|PubMed:9430585, ECO:0000269|PubMed:9495346}.
Interaction P16892:FUS3; NbExp=2; IntAct=EBI-11303, EBI-7193; P87108:TIM10; NbExp=2; IntAct=EBI-11303, EBI-9115;
Similarity Belongs to the small Tim family. {ECO:0000305}.
Subcellular Location Mitochondrion inner membrane {ECO:0000269|PubMed:8663351, ECO:0000269|PubMed:9495346}; Peripheral membrane protein {ECO:0000269|PubMed:8663351, ECO:0000269|PubMed:9495346}.
Subunit Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13). Interacts directly with both the TIM22 protein and the TIM9-TIM10 heterohexamer. Interacts with multi- pass transmembrane proteins in transit. {ECO:0000269|PubMed:10648604, ECO:0000269|PubMed:12637749, ECO:0000269|PubMed:8955274, ECO:0000269|PubMed:9430585, ECO:0000269|PubMed:9495346, ECO:0000269|PubMed:9889188}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007241 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6319567 RefSeq NP_009649 109 Tim12p

Identical Sequences to LMP007241 proteins

Reference Database Accession Length Protein Name
GI:6319567 GenBank EIW12020.1 109 Tim12p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6319567 GenBank EWG87673.1 109 Tim12p [Saccharomyces cerevisiae R008]
GI:6319567 GenBank EWG92448.1 109 Tim12p [Saccharomyces cerevisiae P301]
GI:6319567 GenBank EWG97531.1 109 Tim12p [Saccharomyces cerevisiae R103]
GI:6319567 GenBank EWH19494.1 109 Tim12p [Saccharomyces cerevisiae P283]
GI:6319567 gnl McCuskerlabDuke 109 Tim12p [Saccharomyces cerevisiae YJM993]

Related Sequences to LMP007241 proteins

Reference Database Accession Length Protein Name
GI:6319567 EMBL CCC71793.1 108 hypothetical protein NCAS_0I01250 [Naumovozyma castellii CBS 4309]
GI:6319567 EMBL CCD22929.1 106 hypothetical protein NDAI_0A07750 [Naumovozyma dairenensis CBS 421]
GI:6319567 EMBL CCF59197.1 110 hypothetical protein KAFR_0G01630 [Kazachstania africana CBS 2517]
GI:6319567 GenBank EJT44169.1 109 TIM12-like protein [Saccharomyces kudriavzevii IFO 1802]
GI:6319567 RefSeq XP_003678137.1 108 hypothetical protein NCAS_0I01250 [Naumovozyma castellii CBS 4309]
GI:6319567 RefSeq XP_003958332.1 110 hypothetical protein KAFR_0G01630 [Kazachstania africana CBS 2517]