Gene/Proteome Database (LMPD)

LMPD ID
LMP007253
Gene ID
854342
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
sphinganine kinase LCB4
Gene Symbol
LCB4
Synonyms
-
Alternate Names
sphinganine kinase LCB4
Chromosome
XV
EC Number
2.7.1.91

Proteins

sphinganine kinase LCB4
Refseq ID NP_014814
Protein GI 6324745
UniProt ID Q12246
mRNA ID NM_001183590
Length 624
RefSeq Status PROVISIONAL
MVVQKKLRAILTDEGVLIKSQSHHMFNKHGQLRSGDSLSLLSCLSCLDDGTLSSDGGSFDEDDSLELLPLNTTIPFNRILNAKYVNVGQKGFNNGKISSNPFQTENLSSSSENDDVENHSLSNDKAPVSESQSFPKKDKWDTKTNTVKVSPDDSQDNSPSLGIKDNQQLIELTFAVPKGHDVIPQKLTLLIDHVSRKSRANTGEENISSGTVEEILEKSYENSKRNRSILVIINPHGGKGTAKNLFLTKARPILVESGCKIEIAYTKYARHAIDIAKDLDISKYDTIACASGDGIPYEVINGLYRRPDRVDAFNKLAVTQLPCGSGNAMSISCHWTNNPSYAALCLVKSIETRIDLMCCSQPSYMNEWPRLSFLSQTYGVIAESDINTEFIRWMGPVRFNLGVAFNIIQGKKYPCEVFVKYAAKSKKELKVHFLENKDKNKGCLTFEPNPSPNSSPDLLSKNNINNSTKDELSPNFLNEDNFKLKYPMTEPVPRDWEKMDSELTDNLTIFYTGKMPYIAKDTKFFPAALPADGTIDLVITDARIPVTRMTPILLSLDKGSHVLEPEVIHSKILAYKIIPKVESGLFSVDGEKFPLEPLQVEIMPMLCKTLLRNGRYIDTEFESM

Gene Information

Entrez Gene ID
854342
Gene Name
sphinganine kinase LCB4
Gene Symbol
LCB4
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0032541 IDA:SGD C cortical endoplasmic reticulum
GO:0005783 IDA:SGD C endoplasmic reticulum
GO:0005768 IEA:UniProtKB-KW C endosome
GO:0005794 IDA:SGD C Golgi apparatus
GO:0005886 IDA:SGD C plasma membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0017050 IDA:SGD F D-erythro-sphingosine kinase activity
GO:0004143 IEA:InterPro F diacylglycerol kinase activity
GO:0003951 IEA:InterPro F NAD+ kinase activity
GO:0008481 IEA:UniProtKB-EC F sphinganine kinase activity
GO:0019722 IMP:SGD P calcium-mediated signaling
GO:0007205 IEA:InterPro P protein kinase C-activating G-protein coupled receptor signaling pathway
GO:0006665 IDA:SGD P sphingolipid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
sce00600 Sphingolipid metabolism
sce_M00100 Sphingosine degradation

REACTOME Pathway Links

REACTOME Pathway ID Description
5618486 Association of TriC/CCT with target proteins during biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR016064 ATP-NAD kinase-like domain
IPR001206 Diacylglycerol kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
sphinganine kinase LCB4
Protein Entry
LCB4_YEAST
UniProt ID
Q12246
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=7.7 uM for dihydrosphingosine {ECO:0000269|PubMed:9677363}; KM=25 uM for ATP {ECO:0000269|PubMed:9677363};
Catalytic Activity ATP + phytosphingosine = ADP + phytosphingosine 1-phosphate.
Catalytic Activity ATP + sphinganine = ADP + sphinganine 1- phosphate.
Function Catalyzes the phosphorylation of the sphingoid long chain bases dihydrosphingosine (DHS or sphinganine) and phytosphingosine (PHS) to form dihydrosphingosine 1-phosphate (DHS-1P) and phytosphingosine 1-phosphate (PHS-1P) respectively. Involved in the biosynthesis of sphingolipids and ceramides. Required with LCB3 for an effective incorporation of DHS into ceramides through a phosphorylation-dephosphorylation cycle. Involved in heat-induced transient cell cycle arrest. Accumulation of phosphorylated sphingoid long chain bases (LCBPs) stimulates calcium influx and activates calcineurin signaling. Involved in heat-stress resistance. {ECO:0000269|PubMed:11056159, ECO:0000269|PubMed:11102354, ECO:0000269|PubMed:11278643, ECO:0000269|PubMed:11795842, ECO:0000269|PubMed:11967828, ECO:0000269|PubMed:12493772, ECO:0000269|PubMed:16141212, ECO:0000269|PubMed:9677363}.
Miscellaneous Present with 2840 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Ptm Phosphorylated by the cyclin-CDKs PCL1-PHO85 and PCL2-PHO85. Phosphorylation is a prerequisite to ubiquitination. The phosphorylation level depends on sterol composition and may also be involved in subcellular location. {ECO:0000269|PubMed:15598647, ECO:0000269|PubMed:16141212, ECO:0000269|PubMed:17330950, ECO:0000269|PubMed:18407956, ECO:0000269|PubMed:19779198}.
Ptm Ubiquitinated. The ubiquitination leads to degradation in the vacuole. {ECO:0000269|PubMed:15598647}.
Similarity Contains 1 DAGKc domain. {ECO:0000255|PROSITE- ProRule:PRU00783}.
Subcellular Location Cell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein.

Identical and Related Proteins

Unique RefSeq proteins for LMP007253 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6324745 RefSeq NP_014814 624 sphinganine kinase LCB4

Identical Sequences to LMP007253 proteins

Reference Database Accession Length Protein Name
GI:6324745 EMBL CAA99378.1 624 unnamed protein product [Saccharomyces cerevisiae]
GI:6324745 EMBL CAY86461.1 624 Lcb4p [Saccharomyces cerevisiae EC1118]
GI:6324745 GenBank EGA60486.1 624 Lcb4p [Saccharomyces cerevisiae FostersO]
GI:6324745 GenBank AGF19297.1 624 Sequence 10 from patent US 8367375
GI:6324745 SwissProt Q12246.1 624 RecName: Full=Sphingoid long chain base kinase 4; Short=LCB kinase 4; AltName: Full=Sphinganine kinase 4 [Saccharomyces cerevisiae S288c]
GI:6324745 Third Party Genbank DAA10943.1 624 TPA: sphinganine kinase LCB4 [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007253 proteins

Reference Database Accession Length Protein Name
GI:6324745 GenBank EDZ69241.1 624 YOR171Cp-like protein [Saccharomyces cerevisiae AWRI1631]
GI:6324745 GenBank EEU07743.1 624 Lcb4p [Saccharomyces cerevisiae JAY291]
GI:6324745 GenBank EGA56724.1 624 Lcb4p [Saccharomyces cerevisiae FostersB]
GI:6324745 GenBank EGA80857.1 624 Lcb4p [Saccharomyces cerevisiae Lalvin QA23]
GI:6324745 GenBank EWG88476.1 624 Lcb4p [Saccharomyces cerevisiae P301]
GI:6324745 GenBank EWH15993.1 624 Lcb4p [Saccharomyces cerevisiae P283]