Gene/Proteome Database (LMPD)
LMPD ID
LMP007278
Gene ID
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Gene Symbol
Synonyms
-
Alternate Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Chromosome
VII
EC Number
2.4.99.18
Proteins
| dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 | |
|---|---|
| Refseq ID | NP_011493 |
| Protein GI | 6321416 |
| UniProt ID | P39007 |
| mRNA ID | NM_001180887 |
| Length | 718 |
| RefSeq Status | PROVISIONAL |
| MGSDRSCVLSVFQTILKLVIFVAIFGAAISSRLFAVIKFESIIHEFDPWFNYRATKYLVNNSFYKFLNWFDDRTWYPLGRVTGGTLYPGLMTTSAFIWHALRNWLGLPIDIRNVCVLFAPLFSGVTAWATYEFTKEIKDASAGLLAAGFIAIVPGYISRSVAGSYDNEAIAITLLMVTFMFWIKAQKTGSIMHATCAALFYFYMVSAWGGYVFITNLIPLHVFLLILMGRYSSKLYSAYTTWYAIGTVASMQIPFVGFLPIRSNDHMAALGVFGLIQIVAFGDFVKGQISTAKFKVIMMVSLFLILVLGVVGLSALTYMGLIAPWTGRFYSLWDTNYAKIHIPIIASVSEHQPVSWPAFFFDTHFLIWLFPAGVFLLFLDLKDEHVFVIAYSVLCSYFAGVMVRLMLTLTPVICVSAAVALSKIFDIYLDFKTSDRKYAIKPAALLAKLIVSGSFIFYLYLFVFHSTWVTRTAYSSPSVVLPSQTPDGKLALIDDFREAYYWLRMNSDEDSKVAAWWDYGYQIGGMADRTTLVDNNTWNNTHIAIVGKAMASPEEKSYEILKEHDVDYVLVIFGGLIGFGGDDINKFLWMIRISEGIWPEEIKERDFYTAEGEYRVDARASETMRNSLLYKMSYKDFPQLFNGGQATDRVRQQMITPLDVPPLDYFDEVFTSENWMVRIYQLKKDDAQGRTLRDVGELTRSSTKTRRSIKRPELGLRV | |
Gene Information
Entrez Gene ID
Gene Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Gene Symbol
Species
Saccharomyces cerevisiae S288c
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0008250 | IPI:SGD | C | oligosaccharyltransferase complex |
| GO:0004579 | IC:SGD | F | dolichyl-diphosphooligosaccharide-protein glycotransferase activity |
| GO:0006487 | IPI:SGD | P | protein N-linked glycosylation |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| sce00510 | N-Glycan biosynthesis |
| sce_M00072 | N-glycosylation by oligosaccharyltransferase |
| sce04141 | Protein processing in endoplasmic reticulum |
| sce00513 | Various types of N-glycan biosynthesis |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR003674 | Oligosaccharyl transferase, STT3 subunit |
UniProt Annotations
Entry Information
Gene Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Protein Entry
STT3_YEAST
UniProt ID
Species
Yeast (S288c)
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine. |
| Function | Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. {ECO:0000269|PubMed:12359722}. |
| Interaction | P48439:OST3; NbExp=2; IntAct=EBI-18447, EBI-12680; P32915:SEC61; NbExp=2; IntAct=EBI-18447, EBI-16400; P33767:WBP1; NbExp=3; IntAct=EBI-18447, EBI-12658; |
| Miscellaneous | Present with 3000 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Pathway | Protein modification; protein glycosylation. |
| Similarity | Belongs to the STT3 family. {ECO:0000305}. |
| Subcellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Subunit | Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST3, OST4 and STT3 probably form a subcomplex. May interact directly with OST3, OST4, OST6, WBP1 and SWP1. Interacts with SEC61. {ECO:0000269|PubMed:10677492, ECO:0000269|PubMed:15831493, ECO:0000269|PubMed:15886282, ECO:0000269|PubMed:9405463, ECO:0000269|PubMed:9435788}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007278 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 6321416 | RefSeq | NP_011493 | 718 | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 |
Identical Sequences to LMP007278 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6321416 | DBBJ | GAA23364.1 | 718 | K7_Stt3p [Saccharomyces cerevisiae Kyokai no. 7] |
| GI:6321416 | GenBank | ABC17138.1 | 718 | Sequence 388 from patent US 6949356 |
| GI:6321416 | GenBank | EDN61579.1 | 718 | subunit of the oligosaccharyltransferase complex of the ER lumen [Saccharomyces cerevisiae YJM789] |
| GI:6321416 | GenBank | EEU06314.1 | 718 | Stt3p [Saccharomyces cerevisiae JAY291] |
| GI:6321416 | GenBank | EIW10331.1 | 718 | Stt3p [Saccharomyces cerevisiae CEN.PK113-7D] |
| GI:6321416 | Third Party Genbank | DAA08076.1 | 718 | TPA: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [Saccharomyces cerevisiae S288c] |
Related Sequences to LMP007278 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6321416 | DBBJ | BAA06079.1 | 718 | STT3 protein [Saccharomyces cerevisiae] |
| GI:6321416 | GenBank | EDZ72150.1 | 718 | YGL022Wp-like protein [Saccharomyces cerevisiae AWRI1631] |
| GI:6321416 | GenBank | EWG85878.1 | 718 | Stt3p [Saccharomyces cerevisiae R008] |
| GI:6321416 | GenBank | EWG90973.1 | 718 | Stt3p [Saccharomyces cerevisiae P301] |
| GI:6321416 | GenBank | EWG96077.1 | 718 | Stt3p [Saccharomyces cerevisiae R103] |
| GI:6321416 | GenBank | EWH18253.1 | 718 | Stt3p [Saccharomyces cerevisiae P283] |