LIPID MAPS

LMPD ID

LMP007293

Gene ID

855845

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

trifunctional fatty acid synthase subunit FAS2

Gene Symbol

FAS2

Synonyms

-

Chromosome

XVI

EC Number

2.3.1.86

trifunctional fatty acid synthase subunit FAS2
Refseq ID	NP_015093
Protein GI	6325025
UniProt ID	P19097
mRNA ID	NM_001184045
Length	1887
MKPEVEQELAHILLTELLAYQFASPVRWIETQDVFLKDFNTERVVEIGPSPTLAGMAQRTLKNKYESYDAALSLHREILCYSKDAKEIYYTPDPSELAAKEEPAKEEAPAPTPAASAPAPAAAAPAPVAAAAPAAAAAEIADEPVKASLLLHVLVAHKLKKSLDSIPMSKTIKDLVGGKSTVQNEILGDLGKEFGTTPEKPEETPLEELAETFQDTFSGALGKQSSSLLSRLISSKMPGGFTITVARKYLQTRWGLPSGRQDGVLLVALSNEPAARLGSEADAKAFLDSMAQKYASIVGVDLSSAASASGAAGAGAAAGAAMIDAGALEEITKDHKVLARQQLQVLARYLKMDLDNGERKFLKEKDTVAELQAQLDYLNAELGEFFVNGVATSFSRKKARTFDSSWNWAKQSLLSLYFEIIHGVLKNVDREVVSEAINIMNRSNDALIKFMEYHISNTDETKGENYQLVKTLGEQLIENCKQVLDVDPVYKDVAKPTGPKTAIDKNGNITYSEEPREKVRKLSQYVQEMALGGPITKESQPTIEEDLTRVYKAISAQADKQDISSSTRVEFEKLYSDLMKFLESSKEIDPSQTTQLAGMDVEDALDKDSTKEVASLPNKSTISKTVSSTIPRETIPFLHLRKKTPAGDWKYDRQLSSLFLDGLEKAAFNGVTFKDKYVLITGAGKGSIGAEVLQGLLQGGAKVVVTTSRFSKQVTDYYQSIYAKYGAKGSTLIVVPFNQGSKQDVEALIEFIYDTEKNGGLGWDLDAIIPFAAIPEQGIELEHIDSKSEFAHRIMLTNILRMMGCVKKQKSARGIETRPAQVILPMSPNHGTFGGDGMYSESKLSLETLFNRWHSESWANQLTVCGAIIGWTRGTGLMSANNIIAEGIEKMGVRTFSQKEMAFNLLGLLTPEVVELCQKSPVMADLNGGLQFVPELKEFTAKLRKELVETSEVRKAVSIETALEHKVVNGNSADAAYAQVEIQPRANIQLDFPELKPYKQVKQIAPAELEGLLDLERVIVVTGFAEVGPWGSARTRWEMEAFGEFSLEGCVEMAWIMGFISYHNGNLKGRPYTGWVDSKTKEPVDDKDVKAKYETSILEHSGIRLIEPELFNGYNPEKKEMIQEVIVEEDLEPFEASKETAEQFKHQHGDKVDIFEIPETGEYSVKLLKGATLYIPKALRFDRLVAGQIPTGWNAKTYGISDDIISQVDPITLFVLVSVVEAFIASGITDPYEMYKYVHVSEVGNCSGSGMGGVSALRGMFKDRFKDEPVQNDILQESFINTMSAWVNMLLISSSGPIKTPVGACATSVESVDIGVETILSGKARICIVGGYDDFQEEGSFEFGNMKATSNTLEEFEHGRTPAEMSRPATTTRNGFMEAQGAGIQIIMQADLALKMGVPIYGIVAMAATATDKIGRSVPAPGKGILTTAREHHSSVKYASPNLNMKYRKRQLVTREAQIKDWVENELEALKLEAEEIPSEDQNEFLLERTREIHNEAESQLRAAQQQWGNDFYKRDPRIAPLRGALATYGLTIDDLGVASFHGTSTKANDKNESATINEMMKHLGRSEGNPVIGVFQKFLTGHPKGAAGAWMMNGALQILNSGIIPGNRNADNVDKILEQFEYVLYPSKTLKTDGVRAVSITSFGFGQKGGQAIVVHPDYLYGAITEDRYNEYVAKVSAREKSAYKFFHNGMIYNKLFVSKEHAPYTDELEEDVYLDPLARVSKDKKSGSLTFNSKNIQSKDSYINANTIETAKMIENMTKEKVSNGGVGVDVELITSINVENDTFIERNFTPQEIEYCSAQPSVQSSFAGTWSAKEAVFKSLGVKSLGGGAALKDIEIVRVNKNAPAVELHGNAKKAAEEAGVTDVKVSISHDDLQAVAVAVSTKK

Entrez Gene ID

855845

Gene Name

trifunctional fatty acid synthase subunit FAS2

Gene Symbol

FAS2

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005829	IDA:SGD	C	cytosol
GO:0005835	IDA:SGD	C	fatty acid synthase complex
GO:0004316	IDA:SGD	F	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
GO:0004315	IMP:SGD	F	3-oxoacyl-[acyl-carrier-protein] synthase activity
GO:0004321	IEA:UniProtKB-EC	F	fatty-acyl-CoA synthase activity
GO:0008897	IMP:SGD	F	holo-[acyl-carrier-protein] synthase activity
GO:0000287	IEA:InterPro	F	magnesium ion binding
GO:0042759	IMP:SGD	P	long-chain fatty acid biosynthetic process
GO:0009059	IEA:InterPro	P	macromolecule biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
ko00061	Fatty acid biosynthesis
sce00061	Fatty acid biosynthesis
M00083	Fatty acid biosynthesis, elongation
sce_M00083	Fatty acid biosynthesis, elongation
M00082	Fatty acid biosynthesis, initiation
sce_M00082	Fatty acid biosynthesis, initiation
ko01212	Fatty acid metabolism
sce01212	Fatty acid metabolism
sce01100	Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-5966	fatty acid biosynthesis initiation
PWY-5966	fatty acid biosynthesis initiation II
FASYN-ELONG2-PWY	fatty acid elongation
FASYN-ELONG-PWY-1	fatty acid elongation -- saturated
PWY-5994	palmitate biosynthesis
PWY-5994	palmitate biosynthesis I (animals and fungi)

InterPro Annotations

Accession	Description
IPR008278	4'-phosphopantetheinyl transferase superfamily
IPR002582	ACPS
IPR001227	Acyl transferase domain
IPR016035	Acyl transferase/acyl hydrolase/lysophospholipase
IPR014031	Beta-ketoacyl synthase, C-terminal
IPR014030	Beta-ketoacyl synthase, N-terminal
IPR018201	Beta-ketoacyl synthase, active site
IPR026025	FAS_alpha_yeast
IPR016040	NAD(P)-binding domain
IPR004568	PPantethiene-prot_Trfase_dom
IPR016039	Thiolase-like
IPR016038	Thiolase-like, subgroup

Entry Information

Gene Name

trifunctional fatty acid synthase subunit FAS2

Protein Entry

FAS2_YEAST

UniProt ID

P19097

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Catalytic Activity	Acetyl-CoA + n malonyl-CoA + 2n NADPH = long- chain-acyl-CoA + n CoA + n CO(2) + 2n NADP(+).
Catalytic Activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl- carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein].
Enzyme Regulation	Inhibited by cerulenin by covalent binding to active site of the ketoacyl synthase (KS) region
Enzyme Regulation	Inhibited by cerulenin by covalent binding to active site of the ketoacyl synthase (KS) region. {ECO:0000269\|PubMed:18725634}.
Function	Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3- oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl- carrier-protein] synthase. This subunit coordinates the binding of the six beta subunits to the enzyme complex.
Interaction	P07149:FAS1; NbExp=3; IntAct=EBI-6806, EBI-6795;
Miscellaneous	Present with 17000 molecules/cell in log phase SD medium
Miscellaneous	Present with 17000 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Ptm	4'-phosphopantetheine is transferred from CoA to a specific serine of the Acyl carrier domain by the C-terminal PPT domain. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.
Similarity	Belongs to the fungal fatty acid synthetase subunit alpha family
Similarity	Belongs to the fungal fatty acid synthetase subunit alpha family. {ECO:0000305}.
Similarity	Contains 1 acyl carrier domain
Similarity	Contains 1 acyl carrier domain. {ECO:0000305}.
Subunit	[Alpha(6)beta(6)] hexamers of two multifunctional subunits (alpha and beta). {ECO:0000269\|PubMed:17431182, ECO:0000269\|PubMed:17448991, ECO:0000269\|PubMed:18725634, ECO:0000269\|PubMed:19679086}.